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Influence of different shellfish matrices on the separation of PSP toxins using a postcolumn oxidation liquid chromatography method.

Rey V, Alfonso A, Botana LM, Botana AM - Toxins (Basel) (2015)

Bottom Line: The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information.Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B.Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, Lugo 27002, Spain. veronica.rey@rai.usc.es.

ABSTRACT
The separation of PSP toxins using liquid chromatography with a post-column oxidation fluorescence detection method was performed with different matrices. The separation of PSP toxins depends on several factors, and it is crucial to take into account the presence of interfering matrix peaks to produce a good separation. The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information. Different real shellfish matrices (mussel, scallop, clam and oyster) were studied, and it was seen that the interference is not the same for each individual matrix. It also depends on the species, sampling location and the date of collection. It was proposed that separation should be accomplished taking into account the type of matrix, as well as the concentration of heptane sulfonate in both solvents, since the mobile phase varies regarding the matrix. Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B. For mussel and clam matrices, interfering peaks are not as large as they are in the other group, and the heptane sulfonate concentration was 8.25 mM for both solvents. Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

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Chromatographic separation of dcGTX3-GTX5-dcGTX2, (a) with 11 mM heptane sulfonate in mobile phase; (b) with 8.25 mM heptane sulfonate in mobile phase.
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toxins-07-01324-f001: Chromatographic separation of dcGTX3-GTX5-dcGTX2, (a) with 11 mM heptane sulfonate in mobile phase; (b) with 8.25 mM heptane sulfonate in mobile phase.

Mentions: The age/status of the LC columns has a large impact on the resolution, and the pH of the mobile phase as well as the concentration of the reagents in it are also crucial. The most important component is the ion-pair reagent heptane sulfonate; in our laboratory a better separation was obtained when the concentration of heptane sulfonate was adjusted to 8.25 mM [22]. With these conditions it was possible to separate GTX5 from dcGTX2, as it is shown in Figure 1a (11 mM heptane sulfonate) and Figure 1b (8.25 mM heptane sulfonate). Figure 2 shows the chromatograms of the two working standard solutions after checking how new conditions work for all the standards, where GTXs and STXs are separated in mussel tissue (Figure 2a) and Cs are separated in deionized water (DIW) (Figure 2b).


Influence of different shellfish matrices on the separation of PSP toxins using a postcolumn oxidation liquid chromatography method.

Rey V, Alfonso A, Botana LM, Botana AM - Toxins (Basel) (2015)

Chromatographic separation of dcGTX3-GTX5-dcGTX2, (a) with 11 mM heptane sulfonate in mobile phase; (b) with 8.25 mM heptane sulfonate in mobile phase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4417969&req=5

toxins-07-01324-f001: Chromatographic separation of dcGTX3-GTX5-dcGTX2, (a) with 11 mM heptane sulfonate in mobile phase; (b) with 8.25 mM heptane sulfonate in mobile phase.
Mentions: The age/status of the LC columns has a large impact on the resolution, and the pH of the mobile phase as well as the concentration of the reagents in it are also crucial. The most important component is the ion-pair reagent heptane sulfonate; in our laboratory a better separation was obtained when the concentration of heptane sulfonate was adjusted to 8.25 mM [22]. With these conditions it was possible to separate GTX5 from dcGTX2, as it is shown in Figure 1a (11 mM heptane sulfonate) and Figure 1b (8.25 mM heptane sulfonate). Figure 2 shows the chromatograms of the two working standard solutions after checking how new conditions work for all the standards, where GTXs and STXs are separated in mussel tissue (Figure 2a) and Cs are separated in deionized water (DIW) (Figure 2b).

Bottom Line: The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information.Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B.Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, Lugo 27002, Spain. veronica.rey@rai.usc.es.

ABSTRACT
The separation of PSP toxins using liquid chromatography with a post-column oxidation fluorescence detection method was performed with different matrices. The separation of PSP toxins depends on several factors, and it is crucial to take into account the presence of interfering matrix peaks to produce a good separation. The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information. Different real shellfish matrices (mussel, scallop, clam and oyster) were studied, and it was seen that the interference is not the same for each individual matrix. It also depends on the species, sampling location and the date of collection. It was proposed that separation should be accomplished taking into account the type of matrix, as well as the concentration of heptane sulfonate in both solvents, since the mobile phase varies regarding the matrix. Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B. For mussel and clam matrices, interfering peaks are not as large as they are in the other group, and the heptane sulfonate concentration was 8.25 mM for both solvents. Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

Show MeSH
Related in: MedlinePlus