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Gender-related effects of sex steroids on histamine release and FcεRI expression in rat peritoneal mast cells.

Muñoz-Cruz S, Mendoza-Rodríguez Y, Nava-Castro KE, Yepez-Mulia L, Morales-Montor J - J Immunol Res (2015)

Bottom Line: Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation.In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used.In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.

ABSTRACT
Mast cells (MCs) are versatile effector and regulatory cells in various physiologic, immunologic, and pathologic processes. In addition to the well-characterized IgE/FcεRI-mediated degranulation, a variety of biological substances can induce MCs activation and release of their granule content. Sex steroids, mainly estradiol and progesterone, have been demonstrated to elicit MCs activation. Most published studies have been conducted on MCs lines or freshly isolated peritoneal and bone marrow-derived MC without addressing gender impact on MC response. Our goal was to investigate if the effect of estradiol, progesterone, testosterone, and dihydrotestosterone (DHT) on MCs may differ depending on whether female or male rats are used as MCs donors. Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation. In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used. In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

No MeSH data available.


Related in: MedlinePlus

Differential effect of sex steroids on histamine release by PMCs from male and female rats, immunologically stimulated by the system IgE-DNP. PMCs were pretreated with each hormone at the indicated concentrations and sensitized at the same time with antidinitrophenyl (DNP) IgE (10 μg/mL). PMCs were then stimulated with DNP-HSA (100 ng/mL) for 30 min in the presence of the corresponding hormone. The release of histamine was measured. *P < 0.05 IgE anti-DNP + DNP-HAS plus hormone versus IgE anti-DNP + DNP-HSA alone.
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fig3: Differential effect of sex steroids on histamine release by PMCs from male and female rats, immunologically stimulated by the system IgE-DNP. PMCs were pretreated with each hormone at the indicated concentrations and sensitized at the same time with antidinitrophenyl (DNP) IgE (10 μg/mL). PMCs were then stimulated with DNP-HSA (100 ng/mL) for 30 min in the presence of the corresponding hormone. The release of histamine was measured. *P < 0.05 IgE anti-DNP + DNP-HAS plus hormone versus IgE anti-DNP + DNP-HSA alone.

Mentions: Incubation of IgE anti-DNP-sensitized PMCs with E2, P4, T4, and DHT at different physiological concentrations had different effect on the IgE-DNP mediated histamine release depending on the gender, type, and concentration of the hormone (Figure 3). Pretreatment of PMCs, obtained from female rats, with estradiol at 100 pM and 10 nM significantly increased IgE-DNP mediated histamine secretion. Nevertheless, estradiol pretreatment did not have any effect on the histamine secretion induced by IgE in PMCs from male rats.


Gender-related effects of sex steroids on histamine release and FcεRI expression in rat peritoneal mast cells.

Muñoz-Cruz S, Mendoza-Rodríguez Y, Nava-Castro KE, Yepez-Mulia L, Morales-Montor J - J Immunol Res (2015)

Differential effect of sex steroids on histamine release by PMCs from male and female rats, immunologically stimulated by the system IgE-DNP. PMCs were pretreated with each hormone at the indicated concentrations and sensitized at the same time with antidinitrophenyl (DNP) IgE (10 μg/mL). PMCs were then stimulated with DNP-HSA (100 ng/mL) for 30 min in the presence of the corresponding hormone. The release of histamine was measured. *P < 0.05 IgE anti-DNP + DNP-HAS plus hormone versus IgE anti-DNP + DNP-HSA alone.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417946&req=5

fig3: Differential effect of sex steroids on histamine release by PMCs from male and female rats, immunologically stimulated by the system IgE-DNP. PMCs were pretreated with each hormone at the indicated concentrations and sensitized at the same time with antidinitrophenyl (DNP) IgE (10 μg/mL). PMCs were then stimulated with DNP-HSA (100 ng/mL) for 30 min in the presence of the corresponding hormone. The release of histamine was measured. *P < 0.05 IgE anti-DNP + DNP-HAS plus hormone versus IgE anti-DNP + DNP-HSA alone.
Mentions: Incubation of IgE anti-DNP-sensitized PMCs with E2, P4, T4, and DHT at different physiological concentrations had different effect on the IgE-DNP mediated histamine release depending on the gender, type, and concentration of the hormone (Figure 3). Pretreatment of PMCs, obtained from female rats, with estradiol at 100 pM and 10 nM significantly increased IgE-DNP mediated histamine secretion. Nevertheless, estradiol pretreatment did not have any effect on the histamine secretion induced by IgE in PMCs from male rats.

Bottom Line: Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation.In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used.In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.

ABSTRACT
Mast cells (MCs) are versatile effector and regulatory cells in various physiologic, immunologic, and pathologic processes. In addition to the well-characterized IgE/FcεRI-mediated degranulation, a variety of biological substances can induce MCs activation and release of their granule content. Sex steroids, mainly estradiol and progesterone, have been demonstrated to elicit MCs activation. Most published studies have been conducted on MCs lines or freshly isolated peritoneal and bone marrow-derived MC without addressing gender impact on MC response. Our goal was to investigate if the effect of estradiol, progesterone, testosterone, and dihydrotestosterone (DHT) on MCs may differ depending on whether female or male rats are used as MCs donors. Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation. In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used. In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

No MeSH data available.


Related in: MedlinePlus