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Gender-related effects of sex steroids on histamine release and FcεRI expression in rat peritoneal mast cells.

Muñoz-Cruz S, Mendoza-Rodríguez Y, Nava-Castro KE, Yepez-Mulia L, Morales-Montor J - J Immunol Res (2015)

Bottom Line: Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation.In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used.In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.

ABSTRACT
Mast cells (MCs) are versatile effector and regulatory cells in various physiologic, immunologic, and pathologic processes. In addition to the well-characterized IgE/FcεRI-mediated degranulation, a variety of biological substances can induce MCs activation and release of their granule content. Sex steroids, mainly estradiol and progesterone, have been demonstrated to elicit MCs activation. Most published studies have been conducted on MCs lines or freshly isolated peritoneal and bone marrow-derived MC without addressing gender impact on MC response. Our goal was to investigate if the effect of estradiol, progesterone, testosterone, and dihydrotestosterone (DHT) on MCs may differ depending on whether female or male rats are used as MCs donors. Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation. In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used. In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

No MeSH data available.


Related in: MedlinePlus

Gender and sex steroid concentration effect on histamine release in mast cells. Rat PMCs were treated with physiological (10 pM to 10 nM) and pharmacological concentrations (100 nM to 100 μM) of estradiol, progesterone, testosterone, and DHT for 30 min at 37°C. Values are means ± SEM of three independent experiments in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 female versus male at each hormone concentration. Comparisons between genders were performed by one-way analysis of variance (ANOVA), followed by Bonferroni's multiple comparison test (GraphPad Prism). Basal histamine release was determined in nontreated PMC (no hormone treatment). Hormone concentration and gender had significant effect and the interaction is statistically significant (P < 0.001).
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fig1: Gender and sex steroid concentration effect on histamine release in mast cells. Rat PMCs were treated with physiological (10 pM to 10 nM) and pharmacological concentrations (100 nM to 100 μM) of estradiol, progesterone, testosterone, and DHT for 30 min at 37°C. Values are means ± SEM of three independent experiments in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 female versus male at each hormone concentration. Comparisons between genders were performed by one-way analysis of variance (ANOVA), followed by Bonferroni's multiple comparison test (GraphPad Prism). Basal histamine release was determined in nontreated PMC (no hormone treatment). Hormone concentration and gender had significant effect and the interaction is statistically significant (P < 0.001).

Mentions: E2, P4, T4, and DHT at physiological concentrations caused histamine release (12 to 13.6%) in PMCs from female rats, significantly different (P ≤ 0.05) from the basal release (5 to 6%). In contrast, histamine release from PMCs from male rats was not significantly affected by any of the concentrations of the four hormones and remained similar to the basal release (Figure 1). There was a significant difference on sex steroids-induced histamine release between PMCs from male and female rats (P < 0.01). Furthermore, all the sex steroids tested caused the histamine release on PMC from female rats in a dose-dependent manner.


Gender-related effects of sex steroids on histamine release and FcεRI expression in rat peritoneal mast cells.

Muñoz-Cruz S, Mendoza-Rodríguez Y, Nava-Castro KE, Yepez-Mulia L, Morales-Montor J - J Immunol Res (2015)

Gender and sex steroid concentration effect on histamine release in mast cells. Rat PMCs were treated with physiological (10 pM to 10 nM) and pharmacological concentrations (100 nM to 100 μM) of estradiol, progesterone, testosterone, and DHT for 30 min at 37°C. Values are means ± SEM of three independent experiments in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 female versus male at each hormone concentration. Comparisons between genders were performed by one-way analysis of variance (ANOVA), followed by Bonferroni's multiple comparison test (GraphPad Prism). Basal histamine release was determined in nontreated PMC (no hormone treatment). Hormone concentration and gender had significant effect and the interaction is statistically significant (P < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417946&req=5

fig1: Gender and sex steroid concentration effect on histamine release in mast cells. Rat PMCs were treated with physiological (10 pM to 10 nM) and pharmacological concentrations (100 nM to 100 μM) of estradiol, progesterone, testosterone, and DHT for 30 min at 37°C. Values are means ± SEM of three independent experiments in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001 female versus male at each hormone concentration. Comparisons between genders were performed by one-way analysis of variance (ANOVA), followed by Bonferroni's multiple comparison test (GraphPad Prism). Basal histamine release was determined in nontreated PMC (no hormone treatment). Hormone concentration and gender had significant effect and the interaction is statistically significant (P < 0.001).
Mentions: E2, P4, T4, and DHT at physiological concentrations caused histamine release (12 to 13.6%) in PMCs from female rats, significantly different (P ≤ 0.05) from the basal release (5 to 6%). In contrast, histamine release from PMCs from male rats was not significantly affected by any of the concentrations of the four hormones and remained similar to the basal release (Figure 1). There was a significant difference on sex steroids-induced histamine release between PMCs from male and female rats (P < 0.01). Furthermore, all the sex steroids tested caused the histamine release on PMC from female rats in a dose-dependent manner.

Bottom Line: Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation.In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used.In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Instituto Mexicano del Seguro Social, 06720 México, DF, Mexico.

ABSTRACT
Mast cells (MCs) are versatile effector and regulatory cells in various physiologic, immunologic, and pathologic processes. In addition to the well-characterized IgE/FcεRI-mediated degranulation, a variety of biological substances can induce MCs activation and release of their granule content. Sex steroids, mainly estradiol and progesterone, have been demonstrated to elicit MCs activation. Most published studies have been conducted on MCs lines or freshly isolated peritoneal and bone marrow-derived MC without addressing gender impact on MC response. Our goal was to investigate if the effect of estradiol, progesterone, testosterone, and dihydrotestosterone (DHT) on MCs may differ depending on whether female or male rats are used as MCs donors. Our results demonstrated that effect of sex steroids on MCs histamine release is dose- and gender-dependent and can be direct, synergistic, or inhibitory depending on whether hormones are used alone or to pretreat MCs followed by substance P-stimulation or upon IgE-mediated stimulation. In contrast, sex steroids did not have effect on the MC expression of the IgE high affinity receptor, FcεRI, no matter female or male rats were used. In conclusion, MCs degranulation is modulated by sex hormones in a gender-selective fashion, with MC from females being more susceptible than MC from males to the effects of sex steroids.

No MeSH data available.


Related in: MedlinePlus