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Increased frequency of CD4+ CD25+ FoxP3+ T regulatory cells in pulmonary tuberculosis patients undergoing specific treatment and its relationship with their immune-endocrine profile.

Díaz A, Santucci N, Bongiovanni B, D'Attilio L, Massoni C, Lioi S, Radcliffe S, Dídoli G, Bottasso O, Bay ML - J Immunol Res (2015)

Bottom Line: While IL-6, IFN-γ, TGF-β (ELISA), and Cortisol (electrochemiluminescence, EQ) were augmented, DHEA-S (EQ) levels were diminished at T0 with respect to HCo, with cytokines and Cortisol returning to normal values at T9.Tregs correlated positively with IFN-γ (R = 0.868, P < 0.05) at T2 and negatively at T4 (R = -0.795, P < 0.05).Lowered levels of proinflammatory cytokines together with an increased frequency of Tregs of patients undergoing specific treatment might reflect a downmodulatory effect of these cells on the accompanying inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Rosario, Santa Fe, Argentina.

ABSTRACT
Tuberculosis (TB) is a major health problem requiring an appropriate cell immune response (IR) to be controlled. Since regulatory T cells (Tregs) are relevant in IR regulation, we analyzed Tregs variations throughout the course of TB treatment and its relationship with changes in immune-endocrine mediators dealing with disease immunopathology. The cohort was composed of 41 adult patients, 20 of them completing treatment and follow-up. Patients were bled at diagnosis (T0) and at 2 (T2), 4 (T4), 6 (T6), and 9 months following treatment initiation. Twenty-four age- and sex-matched healthy controls (HCo) were also included. Tregs (flow cytometry) from TB patients were increased at T0 (versus HCo P < 0.05), showing even higher values at T2 (versus T0 P < 0.01) and T4 (versus T0 P < 0.001). While IL-6, IFN-γ, TGF-β (ELISA), and Cortisol (electrochemiluminescence, EQ) were augmented, DHEA-S (EQ) levels were diminished at T0 with respect to HCo, with cytokines and Cortisol returning to normal values at T9. Tregs correlated positively with IFN-γ (R = 0.868, P < 0.05) at T2 and negatively at T4 (R = -0.795, P < 0.05). Lowered levels of proinflammatory cytokines together with an increased frequency of Tregs of patients undergoing specific treatment might reflect a downmodulatory effect of these cells on the accompanying inflammation.

No MeSH data available.


Related in: MedlinePlus

Frequency of CD4+CD25+FoxP3+ T cells (Tregs) within the total CD4+ population of TB patients throughout treatment. Box plots show median values, 25–75 percentiles of data in each group with maximum and minimum values. HCo: healthy controls. T0: time of diagnosis; T2, T4, T6: 2, 4, and 6 months after starting the specific anti-TB treatment; T9: 3 months after the end treatment completion. *HCo versus T0 and T6 P < 0.05; HCo versus T2 and T4 P < 0.01. #T0 versus T2 P < 0.05; T0 versus T4 P < 0.01; ′′T4 versus T9 P < 0.05.
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fig2: Frequency of CD4+CD25+FoxP3+ T cells (Tregs) within the total CD4+ population of TB patients throughout treatment. Box plots show median values, 25–75 percentiles of data in each group with maximum and minimum values. HCo: healthy controls. T0: time of diagnosis; T2, T4, T6: 2, 4, and 6 months after starting the specific anti-TB treatment; T9: 3 months after the end treatment completion. *HCo versus T0 and T6 P < 0.05; HCo versus T2 and T4 P < 0.01. #T0 versus T2 P < 0.05; T0 versus T4 P < 0.01; ′′T4 versus T9 P < 0.05.

Mentions: In earlier studies, we have evaluated immune and endocrine parameters during TB treatment, showing that certain cytokines were augmented at the time of diagnosis (IL-1β, IL-6, and IFN-γ) and even 2 months after treatment initiation (IFN-γ) [15]. In relation to peripheral T cells, we found a decrease in the percentages of CD3+ CD4+ cells at the time of diagnosis that attained normal values upon starting specific treatment (data not shown). When assessing the frequency of Tregs throughout specific treatment, these cell populations remained significantly increased during that period compared to values in HCo, reaching their highest relative levels at T4 (HCo versus T4 P < 0.01, Figure 2).


Increased frequency of CD4+ CD25+ FoxP3+ T regulatory cells in pulmonary tuberculosis patients undergoing specific treatment and its relationship with their immune-endocrine profile.

Díaz A, Santucci N, Bongiovanni B, D'Attilio L, Massoni C, Lioi S, Radcliffe S, Dídoli G, Bottasso O, Bay ML - J Immunol Res (2015)

Frequency of CD4+CD25+FoxP3+ T cells (Tregs) within the total CD4+ population of TB patients throughout treatment. Box plots show median values, 25–75 percentiles of data in each group with maximum and minimum values. HCo: healthy controls. T0: time of diagnosis; T2, T4, T6: 2, 4, and 6 months after starting the specific anti-TB treatment; T9: 3 months after the end treatment completion. *HCo versus T0 and T6 P < 0.05; HCo versus T2 and T4 P < 0.01. #T0 versus T2 P < 0.05; T0 versus T4 P < 0.01; ′′T4 versus T9 P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417597&req=5

fig2: Frequency of CD4+CD25+FoxP3+ T cells (Tregs) within the total CD4+ population of TB patients throughout treatment. Box plots show median values, 25–75 percentiles of data in each group with maximum and minimum values. HCo: healthy controls. T0: time of diagnosis; T2, T4, T6: 2, 4, and 6 months after starting the specific anti-TB treatment; T9: 3 months after the end treatment completion. *HCo versus T0 and T6 P < 0.05; HCo versus T2 and T4 P < 0.01. #T0 versus T2 P < 0.05; T0 versus T4 P < 0.01; ′′T4 versus T9 P < 0.05.
Mentions: In earlier studies, we have evaluated immune and endocrine parameters during TB treatment, showing that certain cytokines were augmented at the time of diagnosis (IL-1β, IL-6, and IFN-γ) and even 2 months after treatment initiation (IFN-γ) [15]. In relation to peripheral T cells, we found a decrease in the percentages of CD3+ CD4+ cells at the time of diagnosis that attained normal values upon starting specific treatment (data not shown). When assessing the frequency of Tregs throughout specific treatment, these cell populations remained significantly increased during that period compared to values in HCo, reaching their highest relative levels at T4 (HCo versus T4 P < 0.01, Figure 2).

Bottom Line: While IL-6, IFN-γ, TGF-β (ELISA), and Cortisol (electrochemiluminescence, EQ) were augmented, DHEA-S (EQ) levels were diminished at T0 with respect to HCo, with cytokines and Cortisol returning to normal values at T9.Tregs correlated positively with IFN-γ (R = 0.868, P < 0.05) at T2 and negatively at T4 (R = -0.795, P < 0.05).Lowered levels of proinflammatory cytokines together with an increased frequency of Tregs of patients undergoing specific treatment might reflect a downmodulatory effect of these cells on the accompanying inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Rosario, Santa Fe, Argentina.

ABSTRACT
Tuberculosis (TB) is a major health problem requiring an appropriate cell immune response (IR) to be controlled. Since regulatory T cells (Tregs) are relevant in IR regulation, we analyzed Tregs variations throughout the course of TB treatment and its relationship with changes in immune-endocrine mediators dealing with disease immunopathology. The cohort was composed of 41 adult patients, 20 of them completing treatment and follow-up. Patients were bled at diagnosis (T0) and at 2 (T2), 4 (T4), 6 (T6), and 9 months following treatment initiation. Twenty-four age- and sex-matched healthy controls (HCo) were also included. Tregs (flow cytometry) from TB patients were increased at T0 (versus HCo P < 0.05), showing even higher values at T2 (versus T0 P < 0.01) and T4 (versus T0 P < 0.001). While IL-6, IFN-γ, TGF-β (ELISA), and Cortisol (electrochemiluminescence, EQ) were augmented, DHEA-S (EQ) levels were diminished at T0 with respect to HCo, with cytokines and Cortisol returning to normal values at T9. Tregs correlated positively with IFN-γ (R = 0.868, P < 0.05) at T2 and negatively at T4 (R = -0.795, P < 0.05). Lowered levels of proinflammatory cytokines together with an increased frequency of Tregs of patients undergoing specific treatment might reflect a downmodulatory effect of these cells on the accompanying inflammation.

No MeSH data available.


Related in: MedlinePlus