Limits...
Impact of a Complex Food Microbiota on Energy Metabolism in the Model Organism Caenorhabditis elegans.

Zanni E, Laudenzi C, Schifano E, Palleschi C, Perozzi G, Uccelletti D, Devirgiliis C - Biomed Res Int (2015)

Bottom Line: Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes.Altered expression of nhr-49, pept-1, and tub-1 genes, associated with obesity phenotypes, was demonstrated by RT-qPCR.Since several pathways are evolutionarily conserved in C. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

ABSTRACT
The nematode Caenorhabditis elegans is widely used as a model system for research on aging, development, and host-pathogen interactions. Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes. We took advantage of C. elegans to evaluate the impact of foodborne microbiota on well characterized physiological features of the worms. Foodborne lactic acid bacteria (LAB) consortium was used to feed nematodes and its composition was evaluated by 16S rDNA analysis and strain typing before and after colonization of the nematode gut. Lactobacillus delbrueckii, L. fermentum, and Leuconostoc lactis were identified as the main species and shown to display different worm gut colonization capacities. LAB supplementation appeared to decrease nematode lifespan compared to the animals fed with the conventional Escherichia coli nutrient source or a probiotic bacterial strain. Reduced brood size was also observed in microbiota-fed nematodes. Moreover, massive accumulation of lipid droplets was revealed by BODIPY staining. Altered expression of nhr-49, pept-1, and tub-1 genes, associated with obesity phenotypes, was demonstrated by RT-qPCR. Since several pathways are evolutionarily conserved in C. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

No MeSH data available.


Related in: MedlinePlus

RT-qPCR analysis of lipid metabolism genes. Expression of (a) nhr-49, (b) tub-1, and (c) pept-1 genes in LGG- or LAB-fed animals after 3 days from hatching. Histograms show the expression of lipid metabolism related genes detected by real-time PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4417589&req=5

fig4: RT-qPCR analysis of lipid metabolism genes. Expression of (a) nhr-49, (b) tub-1, and (c) pept-1 genes in LGG- or LAB-fed animals after 3 days from hatching. Histograms show the expression of lipid metabolism related genes detected by real-time PCR.

Mentions: In order to identify the genes possibly responsible for the alterations induced by the LAB consortium with respect to LGG, real-time qPCR analysis was performed for nhr-49, pept-1, and tub-1 genes, whose involvement in lipid metabolism and obesity-related phenotypes have been described [29–31]. The results showed that the MBC microbiota diet induced an increased transcription of all tested genes with respect to nematodes fed with the LGG diet (Figure 4).


Impact of a Complex Food Microbiota on Energy Metabolism in the Model Organism Caenorhabditis elegans.

Zanni E, Laudenzi C, Schifano E, Palleschi C, Perozzi G, Uccelletti D, Devirgiliis C - Biomed Res Int (2015)

RT-qPCR analysis of lipid metabolism genes. Expression of (a) nhr-49, (b) tub-1, and (c) pept-1 genes in LGG- or LAB-fed animals after 3 days from hatching. Histograms show the expression of lipid metabolism related genes detected by real-time PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417589&req=5

fig4: RT-qPCR analysis of lipid metabolism genes. Expression of (a) nhr-49, (b) tub-1, and (c) pept-1 genes in LGG- or LAB-fed animals after 3 days from hatching. Histograms show the expression of lipid metabolism related genes detected by real-time PCR.
Mentions: In order to identify the genes possibly responsible for the alterations induced by the LAB consortium with respect to LGG, real-time qPCR analysis was performed for nhr-49, pept-1, and tub-1 genes, whose involvement in lipid metabolism and obesity-related phenotypes have been described [29–31]. The results showed that the MBC microbiota diet induced an increased transcription of all tested genes with respect to nematodes fed with the LGG diet (Figure 4).

Bottom Line: Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes.Altered expression of nhr-49, pept-1, and tub-1 genes, associated with obesity phenotypes, was demonstrated by RT-qPCR.Since several pathways are evolutionarily conserved in C. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

ABSTRACT
The nematode Caenorhabditis elegans is widely used as a model system for research on aging, development, and host-pathogen interactions. Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes. We took advantage of C. elegans to evaluate the impact of foodborne microbiota on well characterized physiological features of the worms. Foodborne lactic acid bacteria (LAB) consortium was used to feed nematodes and its composition was evaluated by 16S rDNA analysis and strain typing before and after colonization of the nematode gut. Lactobacillus delbrueckii, L. fermentum, and Leuconostoc lactis were identified as the main species and shown to display different worm gut colonization capacities. LAB supplementation appeared to decrease nematode lifespan compared to the animals fed with the conventional Escherichia coli nutrient source or a probiotic bacterial strain. Reduced brood size was also observed in microbiota-fed nematodes. Moreover, massive accumulation of lipid droplets was revealed by BODIPY staining. Altered expression of nhr-49, pept-1, and tub-1 genes, associated with obesity phenotypes, was demonstrated by RT-qPCR. Since several pathways are evolutionarily conserved in C. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

No MeSH data available.


Related in: MedlinePlus