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IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

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Percentages of Treg and Tr1 cells in PB samples from healthy children (empty circles) and children with metastatic NB (black circles). Data are expressed as % of total cells. Horizontal bars indicated means. P values are indicated where differences are significant.
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fig5: Percentages of Treg and Tr1 cells in PB samples from healthy children (empty circles) and children with metastatic NB (black circles). Data are expressed as % of total cells. Horizontal bars indicated means. P values are indicated where differences are significant.

Mentions: Since we detected increased IL-10 plasma levels in NB patients, we asked whether two regulatory T cell populations able to secrete IL-10, namely, CD4+CD25highCD127low Treg and CD4+CD45R0+CD49b+LAG-3+ Tr1 cells, were also increased. As shown in Figure 5, the percentage of Treg was significantly higher in metastatic NB patients (% of cells: 0.91 ± 0.39) than in age-matched controls (% of cells: 0.32 ± 0.06, P = 0.02), whereas the percentage of Tr1 cells was lower in NB patients (% of cells: 1.1 ± 0.19) than in controls (2.32 ± 0.4, P = 0.01).


IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Percentages of Treg and Tr1 cells in PB samples from healthy children (empty circles) and children with metastatic NB (black circles). Data are expressed as % of total cells. Horizontal bars indicated means. P values are indicated where differences are significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417583&req=5

fig5: Percentages of Treg and Tr1 cells in PB samples from healthy children (empty circles) and children with metastatic NB (black circles). Data are expressed as % of total cells. Horizontal bars indicated means. P values are indicated where differences are significant.
Mentions: Since we detected increased IL-10 plasma levels in NB patients, we asked whether two regulatory T cell populations able to secrete IL-10, namely, CD4+CD25highCD127low Treg and CD4+CD45R0+CD49b+LAG-3+ Tr1 cells, were also increased. As shown in Figure 5, the percentage of Treg was significantly higher in metastatic NB patients (% of cells: 0.91 ± 0.39) than in age-matched controls (% of cells: 0.32 ± 0.06, P = 0.02), whereas the percentage of Tr1 cells was lower in NB patients (% of cells: 1.1 ± 0.19) than in controls (2.32 ± 0.4, P = 0.01).

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

Show MeSH
Related in: MedlinePlus