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IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

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Related in: MedlinePlus

Correlations between IL-10 and FOXP3 (a) and IL-10 and CD163 mRNA (b), in BM samples, and ARG-1 and CD163 (c) and FOXP3 and IL-10 (d) mRNA in PB samples from 41 high-risk NB patients. Data are expressed as 2−ΔCt values.
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fig2: Correlations between IL-10 and FOXP3 (a) and IL-10 and CD163 mRNA (b), in BM samples, and ARG-1 and CD163 (c) and FOXP3 and IL-10 (d) mRNA in PB samples from 41 high-risk NB patients. Data are expressed as 2−ΔCt values.

Mentions: We next analyzed potential correlations in the mRNA expression levels of the four markers in both BM and PB samples. In BM samples, IL-10 expression positively correlated with FOXP3 (r = 0.43; P = 0.0046, Figure 2(a)) and CD163 expression (r = 0.45; P = 0.0028, Figure 2(b)). In PB samples, CD163 correlated with ARG-1 (r = 0.51; P = 0.0005, Figure 2(c)), whereas IL-10 showed an inverse correlation with FOXP3 (r = −0.67; P < 0.0001, Figure 2(d)). These data suggested that IL-10 production in BM may be ascribed to both CD163+ and Treg cells, whereas in PB IL-10 was unlikely produced by FOXP3+ cells. Conversely, in PB, CD163+ myeloid cells may be responsible for ARG-1 production.


IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Correlations between IL-10 and FOXP3 (a) and IL-10 and CD163 mRNA (b), in BM samples, and ARG-1 and CD163 (c) and FOXP3 and IL-10 (d) mRNA in PB samples from 41 high-risk NB patients. Data are expressed as 2−ΔCt values.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417583&req=5

fig2: Correlations between IL-10 and FOXP3 (a) and IL-10 and CD163 mRNA (b), in BM samples, and ARG-1 and CD163 (c) and FOXP3 and IL-10 (d) mRNA in PB samples from 41 high-risk NB patients. Data are expressed as 2−ΔCt values.
Mentions: We next analyzed potential correlations in the mRNA expression levels of the four markers in both BM and PB samples. In BM samples, IL-10 expression positively correlated with FOXP3 (r = 0.43; P = 0.0046, Figure 2(a)) and CD163 expression (r = 0.45; P = 0.0028, Figure 2(b)). In PB samples, CD163 correlated with ARG-1 (r = 0.51; P = 0.0005, Figure 2(c)), whereas IL-10 showed an inverse correlation with FOXP3 (r = −0.67; P < 0.0001, Figure 2(d)). These data suggested that IL-10 production in BM may be ascribed to both CD163+ and Treg cells, whereas in PB IL-10 was unlikely produced by FOXP3+ cells. Conversely, in PB, CD163+ myeloid cells may be responsible for ARG-1 production.

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

Show MeSH
Related in: MedlinePlus