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IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

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Related in: MedlinePlus

Expression of IL-10, FOXP3, ARG-1, and CD163 mRNA in BM (a) and PB (b) samples from 41 high-risk NB patients (black circles) and 20 healthy controls (empty circles). Data are expressed as 2−ΔCt values. Horizontal bars indicated medians. P values are indicated where differences are significant.
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fig1: Expression of IL-10, FOXP3, ARG-1, and CD163 mRNA in BM (a) and PB (b) samples from 41 high-risk NB patients (black circles) and 20 healthy controls (empty circles). Data are expressed as 2−ΔCt values. Horizontal bars indicated medians. P values are indicated where differences are significant.

Mentions: Expression of IL-10, FOXP3, ARG-1, and CD163 mRNAs was analyzed by RT-qPCR in BM and PB samples collected at diagnosis from 41 children with metastatic NB and from 20 healthy donors. As shown in Figure 1(a), the expression of IL-10 mRNA in BM samples was significantly higher in NB patients (2−ΔCt: 1.28 × 10−3 ± 1.32 × 10−3) than in controls (2−ΔCt: 0.41 × 10−3 ± 0.26 × 10−3; P = 0.002), whereas the expression of FOXP3, ARG-1, and CD163 mRNAs was similar between the two groups.


IL-10 and ARG-1 concentrations in bone marrow and peripheral blood of metastatic neuroblastoma patients do not associate with clinical outcome.

Morandi F, Croce M, Cangemi G, Barco S, Rigo V, Carlini B, Amoroso L, Pistoia V, Ferrini S, Corrias MV - J Immunol Res (2015)

Expression of IL-10, FOXP3, ARG-1, and CD163 mRNA in BM (a) and PB (b) samples from 41 high-risk NB patients (black circles) and 20 healthy controls (empty circles). Data are expressed as 2−ΔCt values. Horizontal bars indicated medians. P values are indicated where differences are significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417583&req=5

fig1: Expression of IL-10, FOXP3, ARG-1, and CD163 mRNA in BM (a) and PB (b) samples from 41 high-risk NB patients (black circles) and 20 healthy controls (empty circles). Data are expressed as 2−ΔCt values. Horizontal bars indicated medians. P values are indicated where differences are significant.
Mentions: Expression of IL-10, FOXP3, ARG-1, and CD163 mRNAs was analyzed by RT-qPCR in BM and PB samples collected at diagnosis from 41 children with metastatic NB and from 20 healthy donors. As shown in Figure 1(a), the expression of IL-10 mRNA in BM samples was significantly higher in NB patients (2−ΔCt: 1.28 × 10−3 ± 1.32 × 10−3) than in controls (2−ΔCt: 0.41 × 10−3 ± 0.26 × 10−3; P = 0.002), whereas the expression of FOXP3, ARG-1, and CD163 mRNAs was similar between the two groups.

Bottom Line: Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival.Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found.In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio di Oncologia IRCCS Istituto Giannina Gaslini, 16148 Genova, Italy.

ABSTRACT
The expression of the immunosuppressive molecules IL-10 and arginase 1 (ARG-1), and of FOXP3 and CD163, as markers of regulatory T cells (Treg) and macrophages, respectively, was evaluated in bone marrow (BM) and peripheral blood (PB) samples collected at diagnosis from patients with metastatic neuroblastoma (NB). IL-10 and ARG-1 plasma concentrations were measured and the association of each parameter with patients' outcome was tested. The percentages of immunosuppressive Treg and type-1 regulatory (Tr1) cells were also determined. In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. IL-10 plasma concentration was higher in patients with NB regardless of stage. Neither IL-10 expression nor IL-10 plasma concentration significantly associated with patient survival. In PB samples from metastatic NB patients, ARG-1 and CD163 expression was higher than in controls but their expression did not associate with survival. Moreover, ARG-1 plasma concentration was lower than in controls, and no association with patient outcome was found. Finally, in metastatic NB patients, the percentage of circulating Treg was higher than in controls, whereas that of Tr1 cells was lower. In conclusion, although IL-10 concentration and Treg percentage were increased, their contribution to the natural history of metastatic NB appears uncertain.

Show MeSH
Related in: MedlinePlus