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Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis.

Oleszko A, Olsztyńska-Janus S, Walski T, Grzeszczuk-Kuć K, Bujok J, Gałecka K, Czerski A, Witkiewicz W, Komorowska M - Biomed Res Int (2015)

Bottom Line: Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser.Linear correlation between these methods was shown.A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering and Instrumentation, Faculty of Fundamental Problems of Technology, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland ; Regional Specialist Hospital in Wrocław, Research and Development Centre, Kamieńskiego 73a, 51-124 Wrocław, Poland.

ABSTRACT
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

No MeSH data available.


Related in: MedlinePlus

Dependence of changes in TBARS concentration in a sample upon the concentration of H2O2; mean value relative to control sample ± standard deviation (N = 7).
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fig6: Dependence of changes in TBARS concentration in a sample upon the concentration of H2O2; mean value relative to control sample ± standard deviation (N = 7).

Mentions: A linear change in the TBARS concentrations has been observed relative to the control specimen when H2O2 was added. The results are shown in Figure 6. Therefore, it can be ascertained that the amount of TBARS that were formed is proportional to the amount of agent that causes oxidation in the H2O2 concentration ranges up to 1 mM and has a fixed lipid concentration level in the specimen. Smaller values of standard deviations were recorded than in the case of using FTIR-ATR spectroscopy.


Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis.

Oleszko A, Olsztyńska-Janus S, Walski T, Grzeszczuk-Kuć K, Bujok J, Gałecka K, Czerski A, Witkiewicz W, Komorowska M - Biomed Res Int (2015)

Dependence of changes in TBARS concentration in a sample upon the concentration of H2O2; mean value relative to control sample ± standard deviation (N = 7).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417580&req=5

fig6: Dependence of changes in TBARS concentration in a sample upon the concentration of H2O2; mean value relative to control sample ± standard deviation (N = 7).
Mentions: A linear change in the TBARS concentrations has been observed relative to the control specimen when H2O2 was added. The results are shown in Figure 6. Therefore, it can be ascertained that the amount of TBARS that were formed is proportional to the amount of agent that causes oxidation in the H2O2 concentration ranges up to 1 mM and has a fixed lipid concentration level in the specimen. Smaller values of standard deviations were recorded than in the case of using FTIR-ATR spectroscopy.

Bottom Line: Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser.Linear correlation between these methods was shown.A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering and Instrumentation, Faculty of Fundamental Problems of Technology, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland ; Regional Specialist Hospital in Wrocław, Research and Development Centre, Kamieńskiego 73a, 51-124 Wrocław, Poland.

ABSTRACT
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

No MeSH data available.


Related in: MedlinePlus