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Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities.

Chen ML, Hsieh CC, Chiang BL, Lin BF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS.Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses.IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 1 Changda Road, Gueiren District, Tainan 71101, Taiwan.

ABSTRACT
This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin- (IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4(+) T cells cultures also confirmed that Gt-TRE (5 ~ 50 µg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-κB in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.

No MeSH data available.


Related in: MedlinePlus

The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4+ helper T cells (5 × 105 cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
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fig3: The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4+ helper T cells (5 × 105 cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.

Mentions: To investigate whether triterpenoids and polysaccharides could affect the helper T cells polarization, we isolated CD4+ T cells from the splenocytes of DO11.10 mice to obtain large population of T cells that can be activated by OVA peptide to induce higher antigen-specific cytokines production. The average levels of cytokines secreted from primary CD4+ T cells under stimulation of 1 μg/mL OVA323–339 peptide for 72 hrs from five independent experiments were 41.4 ± 13.0 ng/mL for IL-2, 19.9 ± 11.2 ng/mL for IFNγ, 1.19 ± 0.10 ng/mL for IL-4, and 1.47 ± 1.29 ng/mL for IL-5. The data in Figure 3 was present as the relative cytokine production compared to the 0 μg/mL control cells.


Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities.

Chen ML, Hsieh CC, Chiang BL, Lin BF - Evid Based Complement Alternat Med (2015)

The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4+ helper T cells (5 × 105 cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417579&req=5

fig3: The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4+ helper T cells (5 × 105 cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
Mentions: To investigate whether triterpenoids and polysaccharides could affect the helper T cells polarization, we isolated CD4+ T cells from the splenocytes of DO11.10 mice to obtain large population of T cells that can be activated by OVA peptide to induce higher antigen-specific cytokines production. The average levels of cytokines secreted from primary CD4+ T cells under stimulation of 1 μg/mL OVA323–339 peptide for 72 hrs from five independent experiments were 41.4 ± 13.0 ng/mL for IL-2, 19.9 ± 11.2 ng/mL for IFNγ, 1.19 ± 0.10 ng/mL for IL-4, and 1.47 ± 1.29 ng/mL for IL-5. The data in Figure 3 was present as the relative cytokine production compared to the 0 μg/mL control cells.

Bottom Line: The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS.Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses.IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 1 Changda Road, Gueiren District, Tainan 71101, Taiwan.

ABSTRACT
This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin- (IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4(+) T cells cultures also confirmed that Gt-TRE (5 ~ 50 µg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-κB in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.

No MeSH data available.


Related in: MedlinePlus