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Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities.

Chen ML, Hsieh CC, Chiang BL, Lin BF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS.Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses.IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 1 Changda Road, Gueiren District, Tainan 71101, Taiwan.

ABSTRACT
This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin- (IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4(+) T cells cultures also confirmed that Gt-TRE (5 ~ 50 µg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-κB in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.

No MeSH data available.


Related in: MedlinePlus

The histamine secretion from ionomycin-stimulated RBL-2H3 mast cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. The RBL-2H3 mast cells (5 × 104 cells/mL) were pretreated with Gt-TRE or Gt-PS extracts from G. tsugae for 24 hr, and then stimulated with 500 ng/mL ionomycin for 5 hr. The supernatants were collected and assayed by histamine ELISA kit. The data are representative for three independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
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Related In: Results  -  Collection


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fig1: The histamine secretion from ionomycin-stimulated RBL-2H3 mast cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. The RBL-2H3 mast cells (5 × 104 cells/mL) were pretreated with Gt-TRE or Gt-PS extracts from G. tsugae for 24 hr, and then stimulated with 500 ng/mL ionomycin for 5 hr. The supernatants were collected and assayed by histamine ELISA kit. The data are representative for three independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.

Mentions: Histamine is an important mediator of allergic disease. To evaluate whether triterpenoid-rich extracts or polysaccharide fractions of Ganoderma tsugae could inhibit the histamine secretion on allergic responses [23], histamine release from ionomycin-stimulated RBL-2H3 mast cells cultured with Gt-TRE or Gt-PS was investigated. The results as shown in Figure 1 indicated different effects of Gt-TRE and Gt-PS on histamine productions. Histamine levels were slightly decreased with increased doses of Gt-TRE and significantly suppressed by Gt-TRE at 50 μg/mL. On the contrary, histamine productions were not significantly affected by Gt-PS, though they were tended to increase.


Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities.

Chen ML, Hsieh CC, Chiang BL, Lin BF - Evid Based Complement Alternat Med (2015)

The histamine secretion from ionomycin-stimulated RBL-2H3 mast cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. The RBL-2H3 mast cells (5 × 104 cells/mL) were pretreated with Gt-TRE or Gt-PS extracts from G. tsugae for 24 hr, and then stimulated with 500 ng/mL ionomycin for 5 hr. The supernatants were collected and assayed by histamine ELISA kit. The data are representative for three independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417579&req=5

fig1: The histamine secretion from ionomycin-stimulated RBL-2H3 mast cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. The RBL-2H3 mast cells (5 × 104 cells/mL) were pretreated with Gt-TRE or Gt-PS extracts from G. tsugae for 24 hr, and then stimulated with 500 ng/mL ionomycin for 5 hr. The supernatants were collected and assayed by histamine ELISA kit. The data are representative for three independent experiments. ∗P < 0.05 as compared to the 0 μg/mL control cells.
Mentions: Histamine is an important mediator of allergic disease. To evaluate whether triterpenoid-rich extracts or polysaccharide fractions of Ganoderma tsugae could inhibit the histamine secretion on allergic responses [23], histamine release from ionomycin-stimulated RBL-2H3 mast cells cultured with Gt-TRE or Gt-PS was investigated. The results as shown in Figure 1 indicated different effects of Gt-TRE and Gt-PS on histamine productions. Histamine levels were slightly decreased with increased doses of Gt-TRE and significantly suppressed by Gt-TRE at 50 μg/mL. On the contrary, histamine productions were not significantly affected by Gt-PS, though they were tended to increase.

Bottom Line: The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS.Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses.IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Health Sciences, College of Health Sciences, Chang Jung Christian University, No. 1 Changda Road, Gueiren District, Tainan 71101, Taiwan.

ABSTRACT
This study was to investigate antiallergic effects of triterpenoids (Gt-TRE) and polysaccharide (Gt-PS) extracts from Ganoderma tsugae, using mast cell line RBL-2H3, T cell line EL4, primary T cells, and transfected RAW264.7 macrophage cells. The results showed that histamine secreted from activated RBL-2H3 mast cells was significantly suppressed by Gt-TRE but not Gt-PS. Interleukin- (IL-) 4 secreted from activated EL4 cells was significantly suppressed by Gt-TRE but not Gt-PS. Further primary CD4(+) T cells cultures also confirmed that Gt-TRE (5 ~ 50 µg/mL) significantly suppressed Th2 cytokines IL-4 and IL-5 secretions but had no effect on Th1 cytokines IL-2 and interferon (IFN)-γ. Gt-PS did not affect IL-4 and IL-5 secretions until higher doses (400, 500 µg/mL) and significantly suppressed IFNγ secretions but enhanced IL-2 at these high doses. The reporter gene assay indicated that Gt-TRE inhibited but Gt-PS enhanced the transcriptional activity of NF-κB in activated transfected RAW264.7 cells and transfected EL4 cells. IL-4 secreted by this transfected EL-4 cells was also significantly decreased by Gt-TRE but not by Gt-PS, suggesting that these two fractions may exert different effects on NF-κB related cytokines expression. These data suggested that triterpenoids fraction of Ganoderma tsugae might be the main constituents to alleviate allergic asthma.

No MeSH data available.


Related in: MedlinePlus