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Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test.

Berhane F, Fite A, Daboul N, Al-Janabi W, Msallaty Z, Caruso M, Lewis MK, Yi Z, Diamond MP, Abou-Samra AB, Seyoum B - J Diabetes Res (2015)

Bottom Line: Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05).In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001).In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

View Article: PubMed Central - PubMed

Affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.

ABSTRACT
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

No MeSH data available.


Related in: MedlinePlus

Correlation of triglycerides and mean M-values. Triglyceride levels negatively correlated with M-values.
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fig6: Correlation of triglycerides and mean M-values. Triglyceride levels negatively correlated with M-values.

Mentions: The mean total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels were 183.7 ± 10.9, 102.7 ± 10.3, 49.6 ± 3.2, and 131.7 ± 19.6 mg/dL. There was no correlation between total LDL and M-values; however there was a strong negative correlation between triglycerides and M-values (r = −0.50, P = 0.01). Participants with lower M-value had higher triglycerides (Figure 6). On the contrary, with regard to HDL there was a trend for positive correlation. In addition, those with low M-values had low HDL. There was also strong negative correlation between total cholesterol and M-values (r = −0.50, P = 0.01).


Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test.

Berhane F, Fite A, Daboul N, Al-Janabi W, Msallaty Z, Caruso M, Lewis MK, Yi Z, Diamond MP, Abou-Samra AB, Seyoum B - J Diabetes Res (2015)

Correlation of triglycerides and mean M-values. Triglyceride levels negatively correlated with M-values.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417566&req=5

fig6: Correlation of triglycerides and mean M-values. Triglyceride levels negatively correlated with M-values.
Mentions: The mean total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels were 183.7 ± 10.9, 102.7 ± 10.3, 49.6 ± 3.2, and 131.7 ± 19.6 mg/dL. There was no correlation between total LDL and M-values; however there was a strong negative correlation between triglycerides and M-values (r = −0.50, P = 0.01). Participants with lower M-value had higher triglycerides (Figure 6). On the contrary, with regard to HDL there was a trend for positive correlation. In addition, those with low M-values had low HDL. There was also strong negative correlation between total cholesterol and M-values (r = −0.50, P = 0.01).

Bottom Line: Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05).In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001).In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

View Article: PubMed Central - PubMed

Affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.

ABSTRACT
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

No MeSH data available.


Related in: MedlinePlus