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Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test.

Berhane F, Fite A, Daboul N, Al-Janabi W, Msallaty Z, Caruso M, Lewis MK, Yi Z, Diamond MP, Abou-Samra AB, Seyoum B - J Diabetes Res (2015)

Bottom Line: Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05).In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001).In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

View Article: PubMed Central - PubMed

Affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.

ABSTRACT
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

No MeSH data available.


Related in: MedlinePlus

Plasma lactate values from OGTT according to M-value. OGTT was based on 75 g oral glucose tolerance tested over 120 min. M-values represent the insulin resistance index at time 120 min of HIEC. Plasma lactate was measured as shown in Study Subjects. (a) showed a progressive increase in lactate during OGTT among patients with M-value ≤ 4. The difference is not statistically different but did show a trend. (b) showed the mean total lactate among subjects with M-value greater or less than 4. Participants having M-values above 4 had significantly lower lactate levels (P < 0.01).
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fig4: Plasma lactate values from OGTT according to M-value. OGTT was based on 75 g oral glucose tolerance tested over 120 min. M-values represent the insulin resistance index at time 120 min of HIEC. Plasma lactate was measured as shown in Study Subjects. (a) showed a progressive increase in lactate during OGTT among patients with M-value ≤ 4. The difference is not statistically different but did show a trend. (b) showed the mean total lactate among subjects with M-value greater or less than 4. Participants having M-values above 4 had significantly lower lactate levels (P < 0.01).

Mentions: During OGTT, those subjects with M-values ≤ 4 had slightly higher lactate values at baseline that were maintained throughout the period of OGTT than those with M-value > 4. The difference showed a strong trend but did reach statistical significance (Figure 4(a)). However, the mean total lactate among subjects with M-values less than 4 was significantly higher than those having M-values above 4 (5.2 ± 2.5 mM versus 4.2 ± 1.9 mM, P < 0.01) (Figure 4(b)).


Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test.

Berhane F, Fite A, Daboul N, Al-Janabi W, Msallaty Z, Caruso M, Lewis MK, Yi Z, Diamond MP, Abou-Samra AB, Seyoum B - J Diabetes Res (2015)

Plasma lactate values from OGTT according to M-value. OGTT was based on 75 g oral glucose tolerance tested over 120 min. M-values represent the insulin resistance index at time 120 min of HIEC. Plasma lactate was measured as shown in Study Subjects. (a) showed a progressive increase in lactate during OGTT among patients with M-value ≤ 4. The difference is not statistically different but did show a trend. (b) showed the mean total lactate among subjects with M-value greater or less than 4. Participants having M-values above 4 had significantly lower lactate levels (P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4417566&req=5

fig4: Plasma lactate values from OGTT according to M-value. OGTT was based on 75 g oral glucose tolerance tested over 120 min. M-values represent the insulin resistance index at time 120 min of HIEC. Plasma lactate was measured as shown in Study Subjects. (a) showed a progressive increase in lactate during OGTT among patients with M-value ≤ 4. The difference is not statistically different but did show a trend. (b) showed the mean total lactate among subjects with M-value greater or less than 4. Participants having M-values above 4 had significantly lower lactate levels (P < 0.01).
Mentions: During OGTT, those subjects with M-values ≤ 4 had slightly higher lactate values at baseline that were maintained throughout the period of OGTT than those with M-value > 4. The difference showed a strong trend but did reach statistical significance (Figure 4(a)). However, the mean total lactate among subjects with M-values less than 4 was significantly higher than those having M-values above 4 (5.2 ± 2.5 mM versus 4.2 ± 1.9 mM, P < 0.01) (Figure 4(b)).

Bottom Line: Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05).In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001).In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

View Article: PubMed Central - PubMed

Affiliation: Wayne State University School of Medicine, Detroit, MI 48201, USA.

ABSTRACT
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

No MeSH data available.


Related in: MedlinePlus