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Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis.

Vijayaraghavan P, Primiya RR, Prakash Vincent SG - ISRN Biotechnol (2013)

Bottom Line: The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively.This enzyme was strongly inhibited by 0.005 M of Mn(2+), Mg(2+), and Zn(2+).In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu 629 502, India.

ABSTRACT
A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively. This enzyme was strongly inhibited by 0.005 M of Mn(2+), Mg(2+), and Zn(2+). In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates. Phytase released 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, and 1655 ± 21 mg inorganic phosphate/kg plant phytates, namely, chick pea, corn, green pea, groundnut, pearl pea, and chick feed, respectively.

No MeSH data available.


Related in: MedlinePlus

Growth and production of phytase from Alcaligenes sp. The isolate was inoculated and incubated for 120 h at 37°C. The culture was withdrawn every 12 h, and the growth enzyme activity was determined. The result was the mean of three different repeats. The error bar indicates standard deviation.
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fig1: Growth and production of phytase from Alcaligenes sp. The isolate was inoculated and incubated for 120 h at 37°C. The culture was withdrawn every 12 h, and the growth enzyme activity was determined. The result was the mean of three different repeats. The error bar indicates standard deviation.

Mentions: The Alcaligenes sp. attained maximum growth after 72 h of fermentation, and the absorbance was 1.972 ± 0.036 at 600 nm. The absorbance declined to 1.728 ± 0.027, 1.615 ± 0.039, 1.619 ± 0.781, and 1.407 ± 0.033 at 84, 96, and 108 h of incubation, respectively. The isolate produced an interesting phytase when grown in minimal medium containing sodium phytate as the sole source of phosphorus. The enzyme secretion increased to its maximum of 6.202 ± 0.082 U/mL at 96 h of fermentation. Enzyme production was found to be 0.008 ± 0.005, 1.811 ± 0.221, 4.351 ± 0.192, and 3.610 ± 0.191 U/mL at 24, 48, 72, and 120 h of fermentation, respectively, (Figure 1). In this organism, phytase was expressed on the onset of late log phase. The reduction in enzyme yield after the optimum period was probably due to the depletion of nutrients that are available to microorganism. This result was in accordance with the observation made with Yersinia kristensenii [18].


Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis.

Vijayaraghavan P, Primiya RR, Prakash Vincent SG - ISRN Biotechnol (2013)

Growth and production of phytase from Alcaligenes sp. The isolate was inoculated and incubated for 120 h at 37°C. The culture was withdrawn every 12 h, and the growth enzyme activity was determined. The result was the mean of three different repeats. The error bar indicates standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417553&req=5

fig1: Growth and production of phytase from Alcaligenes sp. The isolate was inoculated and incubated for 120 h at 37°C. The culture was withdrawn every 12 h, and the growth enzyme activity was determined. The result was the mean of three different repeats. The error bar indicates standard deviation.
Mentions: The Alcaligenes sp. attained maximum growth after 72 h of fermentation, and the absorbance was 1.972 ± 0.036 at 600 nm. The absorbance declined to 1.728 ± 0.027, 1.615 ± 0.039, 1.619 ± 0.781, and 1.407 ± 0.033 at 84, 96, and 108 h of incubation, respectively. The isolate produced an interesting phytase when grown in minimal medium containing sodium phytate as the sole source of phosphorus. The enzyme secretion increased to its maximum of 6.202 ± 0.082 U/mL at 96 h of fermentation. Enzyme production was found to be 0.008 ± 0.005, 1.811 ± 0.221, 4.351 ± 0.192, and 3.610 ± 0.191 U/mL at 24, 48, 72, and 120 h of fermentation, respectively, (Figure 1). In this organism, phytase was expressed on the onset of late log phase. The reduction in enzyme yield after the optimum period was probably due to the depletion of nutrients that are available to microorganism. This result was in accordance with the observation made with Yersinia kristensenii [18].

Bottom Line: The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively.This enzyme was strongly inhibited by 0.005 M of Mn(2+), Mg(2+), and Zn(2+).In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu 629 502, India.

ABSTRACT
A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively. This enzyme was strongly inhibited by 0.005 M of Mn(2+), Mg(2+), and Zn(2+). In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates. Phytase released 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, and 1655 ± 21 mg inorganic phosphate/kg plant phytates, namely, chick pea, corn, green pea, groundnut, pearl pea, and chick feed, respectively.

No MeSH data available.


Related in: MedlinePlus