Limits...
Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry.

Sathishkumar T, Baskar R, Aravind M, Tilak S, Deepthi S, Bharathikumar VM - ISRN Biotechnol (2012)

Bottom Line: With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue).The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction.About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641049, India.

ABSTRACT
Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2 ± 0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

No MeSH data available.


Related in: MedlinePlus

HPLC-DAD chromatogram of PTLC eluate 1. Peak 1 corresponds to unknown phenolic compound, peak 2, to dicaffeic acid, and peak 3 to kaempferol-3-O-robinoside-7-O-rhamnoside/robinin.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4417552&req=5

fig5: HPLC-DAD chromatogram of PTLC eluate 1. Peak 1 corresponds to unknown phenolic compound, peak 2, to dicaffeic acid, and peak 3 to kaempferol-3-O-robinoside-7-O-rhamnoside/robinin.

Mentions: The liquid chromatography (LC) of PTLC flower eluate 1 has recorded three peaks A, B and C at a retention time (Rt) of 2.55, 3.54, and 5.27, respectively (Figure 5). The Mass spectra (MS) have confirmed about three different compounds at parent ion m/z 340, 663, and 741 confirming the presence of dihydrocaffeic acid (RA-100%), unknown phenolic compound (RA-100%) and kaempferol-3-O-robinoside-7-O-rhamnoside (robinin) (RA-100%), respectively.


Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry.

Sathishkumar T, Baskar R, Aravind M, Tilak S, Deepthi S, Bharathikumar VM - ISRN Biotechnol (2012)

HPLC-DAD chromatogram of PTLC eluate 1. Peak 1 corresponds to unknown phenolic compound, peak 2, to dicaffeic acid, and peak 3 to kaempferol-3-O-robinoside-7-O-rhamnoside/robinin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417552&req=5

fig5: HPLC-DAD chromatogram of PTLC eluate 1. Peak 1 corresponds to unknown phenolic compound, peak 2, to dicaffeic acid, and peak 3 to kaempferol-3-O-robinoside-7-O-rhamnoside/robinin.
Mentions: The liquid chromatography (LC) of PTLC flower eluate 1 has recorded three peaks A, B and C at a retention time (Rt) of 2.55, 3.54, and 5.27, respectively (Figure 5). The Mass spectra (MS) have confirmed about three different compounds at parent ion m/z 340, 663, and 741 confirming the presence of dihydrocaffeic acid (RA-100%), unknown phenolic compound (RA-100%) and kaempferol-3-O-robinoside-7-O-rhamnoside (robinin) (RA-100%), respectively.

Bottom Line: With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue).The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction.About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641049, India.

ABSTRACT
Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2 ± 0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

No MeSH data available.


Related in: MedlinePlus