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Evaluation of effect of galvanic corrosion between nickel-chromium metal and titanium on ion release and cell toxicity.

Lee JJ, Song KY, Ahn SG, Choi JY, Seo JM, Park JM - J Adv Prosthodont (2015)

Bottom Line: Results of MTT assay were statistically analyzed by the two-way ANOVA test (P<.05).In the cytotoxicity test, the two-way ANOVA showed a significant effect of the alloy type and galvanic corrosion for cytotoxicity (P<.001).The relative cell growth rate (RGR) was decreased further on the groups in contact with titanium (P<.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Prosthodontics and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.

ABSTRACT

Purpose: The purpose of this study was to evaluate cell toxicity due to ion release caused by galvanic corrosion as a result of contact between base metal and titanium.

Materials and methods: It was hypothesized that Nickel (Ni)-Chromium (Cr) alloys with different compositions possess different corrosion resistances when contacted with titanium abutment, and therefore in this study, specimens (10×10×1.5 mm) were fabricated using commercial pure titanium and 3 different types of Ni-Cr alloys (T3, Tilite, Bella bond plus) commonly used for metal ceramic restorations. The specimens were divided into 6 groups according to the composition of Ni-Cr alloy and contact with titanium. The experimental groups were in direct contact with titanium and the control groups were not. After the samples were immersed in the culture medium - Dulbecco's modified Eagle's medium[DMEM] for 48 hours, the released metal ions were detected using inductively coupled plasma mass spectrometer (ICP-MS) and analyzed by the Kruskal-Wallis and Mann-Whitney test (P<.05). Mouse L-929 fibroblast cells were used for cell toxicity evaluation. The cell toxicity of specimens was measured by the 3-{4,5-dimethylthiazol-2yl}-2,5-diphenyltetrazolium bromide (MTT) test. Results of MTT assay were statistically analyzed by the two-way ANOVA test (P<.05). Post-hoc multiple comparisons were conducted using Tukey's tests.

Results: The amount of metal ions released by galvanic corrosion due to contact between the base metal alloy and titanium was increased in all of the specimens. In the cytotoxicity test, the two-way ANOVA showed a significant effect of the alloy type and galvanic corrosion for cytotoxicity (P<.001). The relative cell growth rate (RGR) was decreased further on the groups in contact with titanium (P<.05).

Conclusion: The release of metal ions was increased by galvanic corrosion due to contact between base metal and titanium, and it can cause adverse effects on the tissue around the implant by inducing cytotoxicity.

No MeSH data available.


Related in: MedlinePlus

MTT test of cells exposed to specimens. Standard deviation is indicated. Statistically significant differences is detected at the .05 level, n=10. Same lowercase letters were not statistically significant.
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Figure 2: MTT test of cells exposed to specimens. Standard deviation is indicated. Statistically significant differences is detected at the .05 level, n=10. Same lowercase letters were not statistically significant.

Mentions: Table 4 summarizes the results of the two-way ANOVA, which showed that the effects of alloy type on MTT activity depend on the galvanic corrosion. On analyzing the difference between the groups by two-way ANOVA, the groups in which the alloy was in contact with titanium or without showed a statistically significantly lower RGR compared to that in the negative control group, but they showed a higher RGR than that in the positive control group (P<.05) (Fig 2). The RGR was significantly lower in the group that showed galvanic corrosion due to contact with titanium than in the other group in which the alloy was not in contact with titanium (P<.05). The lowest RGR was measured in the NB+Ti group (71.8 ± 1.7%), followed by the NT+Ti group (78.4 ± 3.4%) and the N+Ti group (87.9 ± 1.4%). The RGR was not significantly different in other groups in which the alloy was not in contact with titanium.


Evaluation of effect of galvanic corrosion between nickel-chromium metal and titanium on ion release and cell toxicity.

Lee JJ, Song KY, Ahn SG, Choi JY, Seo JM, Park JM - J Adv Prosthodont (2015)

MTT test of cells exposed to specimens. Standard deviation is indicated. Statistically significant differences is detected at the .05 level, n=10. Same lowercase letters were not statistically significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414949&req=5

Figure 2: MTT test of cells exposed to specimens. Standard deviation is indicated. Statistically significant differences is detected at the .05 level, n=10. Same lowercase letters were not statistically significant.
Mentions: Table 4 summarizes the results of the two-way ANOVA, which showed that the effects of alloy type on MTT activity depend on the galvanic corrosion. On analyzing the difference between the groups by two-way ANOVA, the groups in which the alloy was in contact with titanium or without showed a statistically significantly lower RGR compared to that in the negative control group, but they showed a higher RGR than that in the positive control group (P<.05) (Fig 2). The RGR was significantly lower in the group that showed galvanic corrosion due to contact with titanium than in the other group in which the alloy was not in contact with titanium (P<.05). The lowest RGR was measured in the NB+Ti group (71.8 ± 1.7%), followed by the NT+Ti group (78.4 ± 3.4%) and the N+Ti group (87.9 ± 1.4%). The RGR was not significantly different in other groups in which the alloy was not in contact with titanium.

Bottom Line: Results of MTT assay were statistically analyzed by the two-way ANOVA test (P<.05).In the cytotoxicity test, the two-way ANOVA showed a significant effect of the alloy type and galvanic corrosion for cytotoxicity (P<.001).The relative cell growth rate (RGR) was decreased further on the groups in contact with titanium (P<.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Prosthodontics and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.

ABSTRACT

Purpose: The purpose of this study was to evaluate cell toxicity due to ion release caused by galvanic corrosion as a result of contact between base metal and titanium.

Materials and methods: It was hypothesized that Nickel (Ni)-Chromium (Cr) alloys with different compositions possess different corrosion resistances when contacted with titanium abutment, and therefore in this study, specimens (10×10×1.5 mm) were fabricated using commercial pure titanium and 3 different types of Ni-Cr alloys (T3, Tilite, Bella bond plus) commonly used for metal ceramic restorations. The specimens were divided into 6 groups according to the composition of Ni-Cr alloy and contact with titanium. The experimental groups were in direct contact with titanium and the control groups were not. After the samples were immersed in the culture medium - Dulbecco's modified Eagle's medium[DMEM] for 48 hours, the released metal ions were detected using inductively coupled plasma mass spectrometer (ICP-MS) and analyzed by the Kruskal-Wallis and Mann-Whitney test (P<.05). Mouse L-929 fibroblast cells were used for cell toxicity evaluation. The cell toxicity of specimens was measured by the 3-{4,5-dimethylthiazol-2yl}-2,5-diphenyltetrazolium bromide (MTT) test. Results of MTT assay were statistically analyzed by the two-way ANOVA test (P<.05). Post-hoc multiple comparisons were conducted using Tukey's tests.

Results: The amount of metal ions released by galvanic corrosion due to contact between the base metal alloy and titanium was increased in all of the specimens. In the cytotoxicity test, the two-way ANOVA showed a significant effect of the alloy type and galvanic corrosion for cytotoxicity (P<.001). The relative cell growth rate (RGR) was decreased further on the groups in contact with titanium (P<.05).

Conclusion: The release of metal ions was increased by galvanic corrosion due to contact between base metal and titanium, and it can cause adverse effects on the tissue around the implant by inducing cytotoxicity.

No MeSH data available.


Related in: MedlinePlus