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Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti1-xZrx)N coating on titanium.

Ji MK, Park SW, Lee K, Kang IC, Yun KD, Kim HS, Lim HP - J Adv Prosthodont (2015)

Bottom Line: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).The number of P. gingivalis colonies on all surfaces showed no significant differences.TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system.

Materials and methods: Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction.

Results: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).

Conclusion: The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

No MeSH data available.


Reverse transcription-polymerase chain reaction measurements of osteonectin, integrin β1, and β-actin mRNA levels from MG-63 cells. (a) polished-Ti, (b) TiN, (c) ZrN, (d) (Ti75Zr25)N (e) (Ti50Zr50)N (f) (Ti25Zr75)N.
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Figure 5: Reverse transcription-polymerase chain reaction measurements of osteonectin, integrin β1, and β-actin mRNA levels from MG-63 cells. (a) polished-Ti, (b) TiN, (c) ZrN, (d) (Ti75Zr25)N (e) (Ti50Zr50)N (f) (Ti25Zr75)N.

Mentions: The osteoblast-like cell viability values were slightly lower or higher for the TiN, ZrN and (Ti1-xZrx)N coated specimens than those for polished-Ti. However, no significant difference was observed among the six groups (Fig. 4). Osteonectin, integrin-β1, and β-actin mRNA expression is shown in Fig. 5. As a result of the RT-PCR measurement, no significant differences were observed between the levels of osteonectin, integrin-β1, or β-actin mRNA in cells on all specimens.


Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti1-xZrx)N coating on titanium.

Ji MK, Park SW, Lee K, Kang IC, Yun KD, Kim HS, Lim HP - J Adv Prosthodont (2015)

Reverse transcription-polymerase chain reaction measurements of osteonectin, integrin β1, and β-actin mRNA levels from MG-63 cells. (a) polished-Ti, (b) TiN, (c) ZrN, (d) (Ti75Zr25)N (e) (Ti50Zr50)N (f) (Ti25Zr75)N.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414948&req=5

Figure 5: Reverse transcription-polymerase chain reaction measurements of osteonectin, integrin β1, and β-actin mRNA levels from MG-63 cells. (a) polished-Ti, (b) TiN, (c) ZrN, (d) (Ti75Zr25)N (e) (Ti50Zr50)N (f) (Ti25Zr75)N.
Mentions: The osteoblast-like cell viability values were slightly lower or higher for the TiN, ZrN and (Ti1-xZrx)N coated specimens than those for polished-Ti. However, no significant difference was observed among the six groups (Fig. 4). Osteonectin, integrin-β1, and β-actin mRNA expression is shown in Fig. 5. As a result of the RT-PCR measurement, no significant differences were observed between the levels of osteonectin, integrin-β1, or β-actin mRNA in cells on all specimens.

Bottom Line: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).The number of P. gingivalis colonies on all surfaces showed no significant differences.TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system.

Materials and methods: Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction.

Results: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).

Conclusion: The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

No MeSH data available.