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Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti1-xZrx)N coating on titanium.

Ji MK, Park SW, Lee K, Kang IC, Yun KD, Kim HS, Lim HP - J Adv Prosthodont (2015)

Bottom Line: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).The number of P. gingivalis colonies on all surfaces showed no significant differences.TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system.

Materials and methods: Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction.

Results: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).

Conclusion: The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

No MeSH data available.


Schematic diagram of AIP system.
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Figure 1: Schematic diagram of AIP system.

Mentions: Commercially pure titanium (ASTM Grade II, Kobe Steel Co., Kobe, Japan) were used as the substrate. Specimen discs size is 15 mm in diameter and 3 mm in thickness. Discs surface were ground down to 1200 grit SiC paper under water, then polished using 0.3 µm alumina suspension. Discs were then cleaned with acetone, ethyl alcohol and distilled water for 10min in an ultrasonic cleaner. TiN, ZrN and (Ti1-xZrx)N coating was performed with the arc ion plating system (ATS-MC-STD-300; ATECH System, Seoul, Korea) for 60 min, -100V, with N2 at a flow rate of 110 sccm (standard cubic centimeters per minute) (Fig. 1). Experimental groups were TiN, ZrN, (Ti75Zr25)N, (Ti50Zr50) N and (Ti25Zr75)N. Control group was polished-Ti. All specimens (n=346) were ultrasonically cleaned with acetone, ethyl alcohol and distilled water and were sterilized with ethylene oxide gas.


Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and (Ti1-xZrx)N coating on titanium.

Ji MK, Park SW, Lee K, Kang IC, Yun KD, Kim HS, Lim HP - J Adv Prosthodont (2015)

Schematic diagram of AIP system.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414948&req=5

Figure 1: Schematic diagram of AIP system.
Mentions: Commercially pure titanium (ASTM Grade II, Kobe Steel Co., Kobe, Japan) were used as the substrate. Specimen discs size is 15 mm in diameter and 3 mm in thickness. Discs surface were ground down to 1200 grit SiC paper under water, then polished using 0.3 µm alumina suspension. Discs were then cleaned with acetone, ethyl alcohol and distilled water for 10min in an ultrasonic cleaner. TiN, ZrN and (Ti1-xZrx)N coating was performed with the arc ion plating system (ATS-MC-STD-300; ATECH System, Seoul, Korea) for 60 min, -100V, with N2 at a flow rate of 110 sccm (standard cubic centimeters per minute) (Fig. 1). Experimental groups were TiN, ZrN, (Ti75Zr25)N, (Ti50Zr50) N and (Ti25Zr75)N. Control group was polished-Ti. All specimens (n=346) were ultrasonically cleaned with acetone, ethyl alcohol and distilled water and were sterilized with ethylene oxide gas.

Bottom Line: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).The number of P. gingivalis colonies on all surfaces showed no significant differences.TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.

ABSTRACT

Purpose: The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system.

Materials and methods: Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction.

Results: The number of S. mutans colonies on the TiN, ZrN and (Ti1-xZrx)N coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05).

Conclusion: The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and (Ti1-xZrx)N coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

No MeSH data available.