Limits...
Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus

(A) Adherence of epithelial cells (YD-38) cultured on titanium discs for 1 and 3 days. No significant difference was observed in the adhesion strength between SM, SM15, and SM30 discs on day 1. SM: Smooth surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. (B) Cell adherence of YD-38 cells cultured on titanium discs for 3 days. **P<.01, SM vs. SM15, SM vs. SM30. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4414944&req=5

Figure 6: (A) Adherence of epithelial cells (YD-38) cultured on titanium discs for 1 and 3 days. No significant difference was observed in the adhesion strength between SM, SM15, and SM30 discs on day 1. SM: Smooth surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. (B) Cell adherence of YD-38 cells cultured on titanium discs for 3 days. **P<.01, SM vs. SM15, SM vs. SM30. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.

Mentions: Adhesion assays of YD-38 cells showed no significant differences in adhesion strength among SM, SM15, and SM30 discs on day 1. However, the numbers of adherent YD-38 cells on SM15 and SM30 discs were significantly higher than that on SM discs on day 3 (Fig. 6A). SM15 and SM30 discs had 1.2 times (P<.01, SM15 vs. SM) and 1.5 times (P<.01, SM30 vs. SM) higher number of attached YD-38 cells, respectively, compared to that on SM discs (Fig. 6B). However, the adhesion strength of MRC-5 cell cultures on all discs showed no statistical difference after 1 or 3 days (Fig. 7).


Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

(A) Adherence of epithelial cells (YD-38) cultured on titanium discs for 1 and 3 days. No significant difference was observed in the adhesion strength between SM, SM15, and SM30 discs on day 1. SM: Smooth surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. (B) Cell adherence of YD-38 cells cultured on titanium discs for 3 days. **P<.01, SM vs. SM15, SM vs. SM30. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414944&req=5

Figure 6: (A) Adherence of epithelial cells (YD-38) cultured on titanium discs for 1 and 3 days. No significant difference was observed in the adhesion strength between SM, SM15, and SM30 discs on day 1. SM: Smooth surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. (B) Cell adherence of YD-38 cells cultured on titanium discs for 3 days. **P<.01, SM vs. SM15, SM vs. SM30. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
Mentions: Adhesion assays of YD-38 cells showed no significant differences in adhesion strength among SM, SM15, and SM30 discs on day 1. However, the numbers of adherent YD-38 cells on SM15 and SM30 discs were significantly higher than that on SM discs on day 3 (Fig. 6A). SM15 and SM30 discs had 1.2 times (P<.01, SM15 vs. SM) and 1.5 times (P<.01, SM30 vs. SM) higher number of attached YD-38 cells, respectively, compared to that on SM discs (Fig. 6B). However, the adhesion strength of MRC-5 cell cultures on all discs showed no statistical difference after 1 or 3 days (Fig. 7).

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus