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Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus

Proliferation of fibroblast cells (MRC-5) cultured on titanium discs for 3 days. SM: Smooth Surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
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Figure 5: Proliferation of fibroblast cells (MRC-5) cultured on titanium discs for 3 days. SM: Smooth Surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.

Mentions: The number of YD-38 cells increased from day 1 to day 3 by 1.7-fold on SM discs, by 1.9-fold on SM15 discs, and by 2-fold on SM30 discs (Fig. 4). The number of YD-38 cells was the highest when cultured on SM30 discs; however, the differences in cell proliferation among the 3 types of discs were not statistically significant. The proliferation of MRC-5 cells cultured on any of the discs for 3 days showed no significant differences (Fig. 5).


Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

Proliferation of fibroblast cells (MRC-5) cultured on titanium discs for 3 days. SM: Smooth Surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414944&req=5

Figure 5: Proliferation of fibroblast cells (MRC-5) cultured on titanium discs for 3 days. SM: Smooth Surface, served as a control, SM15: 5-µm dimples and 15-µm center distance, and SM30: 5-µm dimples and 30-µm center distance. The value was assumed 100 in control disc at day 1. All values are expressed as percentage.
Mentions: The number of YD-38 cells increased from day 1 to day 3 by 1.7-fold on SM discs, by 1.9-fold on SM15 discs, and by 2-fold on SM30 discs (Fig. 4). The number of YD-38 cells was the highest when cultured on SM30 discs; however, the differences in cell proliferation among the 3 types of discs were not statistically significant. The proliferation of MRC-5 cells cultured on any of the discs for 3 days showed no significant differences (Fig. 5).

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus