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Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus

Light microscopy images of each group (40× magnification). A, SM15: 5-µm dimple and 15-µm center distance. B, SM30: 5-µm dimple and 30-µm center distance.
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Figure 3: Light microscopy images of each group (40× magnification). A, SM15: 5-µm dimple and 15-µm center distance. B, SM30: 5-µm dimple and 30-µm center distance.

Mentions: Dimples of 5-µm diameter (Fig. 1) were formed using a 220 fs-pulsed Ti: sapphire laser (wavelength: 800 nm; repetition rate: 100 kHz). The laser beam was focused by a magnification microscope objective IR lens (×20, NA 0.4; Olympus, Tokyo, Japan) by using a laser power of 5 mW and a laser irradiation time of 1 ms to form a dimple. The sample was moved with a velocity of 1 mm/s. Microdimpling was performed on a 2 × 4 mm2 area at the center of the polished Ti discs (Fig. 2). Before irradiation, the sample surface was cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing with 0.1 µm Alumina powder. The discs were divided into three groups: SM (n = 16) discs were polished Ti discs with no dimples, which served as controls, SM15 (n = 16) discs had 5-µm dimples at 10-µm intervals (center distance; pitch = 15 µm), and SM30 (n = 16) discs had 5-µm dimples at 25-µm intervals (center distance; pitch = 30 µm) in the polished disc (Fig. 3).


Effect of laser-dimpled titanium surfaces on attachment of epithelial-like cells and fibroblasts.

Lee DW, Kim JG, Kim MK, Ansari S, Moshaverinia A, Choi SH, Ryu JJ - J Adv Prosthodont (2015)

Light microscopy images of each group (40× magnification). A, SM15: 5-µm dimple and 15-µm center distance. B, SM30: 5-µm dimple and 30-µm center distance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414944&req=5

Figure 3: Light microscopy images of each group (40× magnification). A, SM15: 5-µm dimple and 15-µm center distance. B, SM30: 5-µm dimple and 30-µm center distance.
Mentions: Dimples of 5-µm diameter (Fig. 1) were formed using a 220 fs-pulsed Ti: sapphire laser (wavelength: 800 nm; repetition rate: 100 kHz). The laser beam was focused by a magnification microscope objective IR lens (×20, NA 0.4; Olympus, Tokyo, Japan) by using a laser power of 5 mW and a laser irradiation time of 1 ms to form a dimple. The sample was moved with a velocity of 1 mm/s. Microdimpling was performed on a 2 × 4 mm2 area at the center of the polished Ti discs (Fig. 2). Before irradiation, the sample surface was cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing with 0.1 µm Alumina powder. The discs were divided into three groups: SM (n = 16) discs were polished Ti discs with no dimples, which served as controls, SM15 (n = 16) discs had 5-µm dimples at 10-µm intervals (center distance; pitch = 15 µm), and SM30 (n = 16) discs had 5-µm dimples at 25-µm intervals (center distance; pitch = 30 µm) in the polished disc (Fig. 3).

Bottom Line: These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc.These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier.This study provided helpful information for developing the transmucosal surface of the abutment.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Veterans Health Service Medical Center, Seoul, Republic of Korea; Department of Dentistry, Graduate School, Korea University, Seoul, Republic of Korea.

ABSTRACT

Purpose: The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.

Materials and methods: Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm(2) area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences.

Results: The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins.

Conclusion: These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.

No MeSH data available.


Related in: MedlinePlus