Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology.
Bottom Line: The primary function of this protein is to encapsidate the viral RNA genome, and it is also thought to participate in the modulation of host cell biology and recruitment of cellular factors to facilitate virus infection.In order to the better understand these latter roles the cellular interactome of PRRSV N protein was defined using label free quantitative proteomics.This identified several cellular factors that could interact with the N protein including poly [ADP-ribose] polymerase 1 (PARP-1), a cellular protein, which can add adenosine diphosphate ribose to a protein.
Affiliation: College of Life Sciences, Northwest A&F University, Yangling, China.Show MeSH
Related in: MedlinePlus
Mentions: Different doses of 3-AB were assessed for potential anti-viral activity by infecting cells with the same MOI of PRRSV and then treating cells with 5, 10, and 20 mM 3-AB over the infection period. Progeny virus production was compared by endpoint dilution assay to an untreated control at 12, 24, 48, 72 and 96 h post-infection. The data indicated that at the 12, 24 and 48 h time points, using a one-way ANOVA test, there was no significant difference (p > 0.05) in progeny virus production, however at 72 and 96 h post-infection there was a significant difference (p < 0.05) in progeny virus production between the treatments compared to the control non-treated infected cells (Fig. 4B). This was also confirmed using indirect immunofluorescence microscopy to detect viral antigens in virus-infected cells at 24, 48, 72 and 96 h post-infection compared to an untreated control (Fig. 5A). There were considerably less foci of infection in the treated cells compared to untreated control infected cells (Fig. 5B) (data shown for the 20 mM concentration of 3-AB). This confirmed the titration data that virus biology was adversely affected by 3-AB, with virus output being reduced to 1–2 log10 at 96 h post-infection compared to 7 log10 for the infected, but untreated control (Fig. 4B).
Affiliation: College of Life Sciences, Northwest A&F University, Yangling, China.