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The Effect of Umbilical Cord Blood Derived Mesenchymal Stem Cells in Monocrotaline-induced Pulmonary Artery Hypertension Rats.

Lee H, Lee JC, Kwon JH, Kim KC, Cho MS, Yang YS, Oh W, Choi SJ, Seo ES, Lee SJ, Wang TJ, Hong YM - J. Korean Med. Sci. (2015)

Bottom Line: The indicators of RV hypertrophy were significantly reduced in the U group at week 4.Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4.The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)-induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.

No MeSH data available.


Related in: MedlinePlus

Engraftment of hUCB-MSCs in lung tissues. The hUCB-MSCs tagged by PKh-26 are observed at 1,050 nm wave length in the lung tissues. Light circular forms are considered to UCB-MSCs engrafted. C, control group; M, monocrotaline group; U, hUCB-MSCs group (Field of view: 300 µm).
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Figure 2: Engraftment of hUCB-MSCs in lung tissues. The hUCB-MSCs tagged by PKh-26 are observed at 1,050 nm wave length in the lung tissues. Light circular forms are considered to UCB-MSCs engrafted. C, control group; M, monocrotaline group; U, hUCB-MSCs group (Field of view: 300 µm).

Mentions: We confirmed that the hUCB-MSCs were engrafted in the lung tissues using two-photon microscopy. In the U group, white circular forms were observed at 1,050 nm wave length which could be hUCB-MSCs. These white circular forms in the U group were more lighter in color compared than those in both C and M group (Fig. 2). PKH-26 detection % area was greater in the U group than the C or M group (C vs. M vs. U; 1.00 vs. 0.87 vs. 1.43)


The Effect of Umbilical Cord Blood Derived Mesenchymal Stem Cells in Monocrotaline-induced Pulmonary Artery Hypertension Rats.

Lee H, Lee JC, Kwon JH, Kim KC, Cho MS, Yang YS, Oh W, Choi SJ, Seo ES, Lee SJ, Wang TJ, Hong YM - J. Korean Med. Sci. (2015)

Engraftment of hUCB-MSCs in lung tissues. The hUCB-MSCs tagged by PKh-26 are observed at 1,050 nm wave length in the lung tissues. Light circular forms are considered to UCB-MSCs engrafted. C, control group; M, monocrotaline group; U, hUCB-MSCs group (Field of view: 300 µm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414641&req=5

Figure 2: Engraftment of hUCB-MSCs in lung tissues. The hUCB-MSCs tagged by PKh-26 are observed at 1,050 nm wave length in the lung tissues. Light circular forms are considered to UCB-MSCs engrafted. C, control group; M, monocrotaline group; U, hUCB-MSCs group (Field of view: 300 µm).
Mentions: We confirmed that the hUCB-MSCs were engrafted in the lung tissues using two-photon microscopy. In the U group, white circular forms were observed at 1,050 nm wave length which could be hUCB-MSCs. These white circular forms in the U group were more lighter in color compared than those in both C and M group (Fig. 2). PKH-26 detection % area was greater in the U group than the C or M group (C vs. M vs. U; 1.00 vs. 0.87 vs. 1.43)

Bottom Line: The indicators of RV hypertrophy were significantly reduced in the U group at week 4.Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4.The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)-induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.

No MeSH data available.


Related in: MedlinePlus