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Cross-reactivity of Toxocariasis with Crude Antigen of Toxascaris leonina Larvae by ELISA.

Jin Y, Shen C, Huh S, Choi MH, Hong ST - J. Korean Med. Sci. (2015)

Bottom Line: Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world.Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers.Most of them showed high absorbances with TCLA.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. Recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in Korea. The present study investigated serological cross-reactivity between crude antigens of T. canis (TCLA) and T. leonina (TLLA) larvae. We collected serum specimens from 177 toxocariasis patients who were clinically suspected in the Seoul National University Hospital and 115 healthy controls. An ELISA method for toxocariasis was used to evaluate diagnostic efficacy of TLLA for serodiagnosis of human toxocariasis. The IgG ELISA using TLLA gave 14 (14.3%) positives of 98 TCLA positive specimens among 177 suspected toxocariasis patients. Most of them showed high absorbances with TCLA. In conclusion, there is a partial cross reaction between serum specimens of toxocariasis and TLLA.

No MeSH data available.


Related in: MedlinePlus

Distribution of ELISA absorbances of clinical toxocariasis and healthy control with TCLA and TLLA. TCLA, crude antigen of Toxocara canis larvae; TLLA, crude antigen of Toxascaris leonina. Horizontal bars represent the mean values.
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Figure 1: Distribution of ELISA absorbances of clinical toxocariasis and healthy control with TCLA and TLLA. TCLA, crude antigen of Toxocara canis larvae; TLLA, crude antigen of Toxascaris leonina. Horizontal bars represent the mean values.

Mentions: The mean absorbances of IgG ELISA using TCLA and TLLA from serum samples of clinically diagnosed toxocariasis and healthy control are presented in Fig. 1. Of the 177 clinical toxocariasis patients, 98 were positive by TCLA ELISA and 14 were positive by TLLA ELISA. The IgG ELISA using TLLA gave 14.3% (14/98) positivity for toxocariasis as shown in Table 1. These data exhibited low diagnostic value with TLLA.


Cross-reactivity of Toxocariasis with Crude Antigen of Toxascaris leonina Larvae by ELISA.

Jin Y, Shen C, Huh S, Choi MH, Hong ST - J. Korean Med. Sci. (2015)

Distribution of ELISA absorbances of clinical toxocariasis and healthy control with TCLA and TLLA. TCLA, crude antigen of Toxocara canis larvae; TLLA, crude antigen of Toxascaris leonina. Horizontal bars represent the mean values.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414637&req=5

Figure 1: Distribution of ELISA absorbances of clinical toxocariasis and healthy control with TCLA and TLLA. TCLA, crude antigen of Toxocara canis larvae; TLLA, crude antigen of Toxascaris leonina. Horizontal bars represent the mean values.
Mentions: The mean absorbances of IgG ELISA using TCLA and TLLA from serum samples of clinically diagnosed toxocariasis and healthy control are presented in Fig. 1. Of the 177 clinical toxocariasis patients, 98 were positive by TCLA ELISA and 14 were positive by TLLA ELISA. The IgG ELISA using TLLA gave 14.3% (14/98) positivity for toxocariasis as shown in Table 1. These data exhibited low diagnostic value with TLLA.

Bottom Line: Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world.Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers.Most of them showed high absorbances with TCLA.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul, Korea.

ABSTRACT
Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated environments or larvae by consumption of raw or uncooked meat or livers. Recently, patients of clinically diagnosed toxocariasis are increasing and require correct diagnosis in Korea. The present study investigated serological cross-reactivity between crude antigens of T. canis (TCLA) and T. leonina (TLLA) larvae. We collected serum specimens from 177 toxocariasis patients who were clinically suspected in the Seoul National University Hospital and 115 healthy controls. An ELISA method for toxocariasis was used to evaluate diagnostic efficacy of TLLA for serodiagnosis of human toxocariasis. The IgG ELISA using TLLA gave 14 (14.3%) positives of 98 TCLA positive specimens among 177 suspected toxocariasis patients. Most of them showed high absorbances with TCLA. In conclusion, there is a partial cross reaction between serum specimens of toxocariasis and TLLA.

No MeSH data available.


Related in: MedlinePlus