Limits...
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures.

Kim SH, Lee WS, Lee NM, Chae SA, Yun SW - Korean J Pediatr (2015)

Bottom Line: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant.In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.

Methods: We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.

Results: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).

Conclusion: Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.

No MeSH data available.


Related in: MedlinePlus

Percentage of cell death in the 7 days in vitro group, as measured by propidium iodide fluorescence. CA, cornu ammonis; DG, dentate gyrus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4414629&req=5

Figure 3: Percentage of cell death in the 7 days in vitro group, as measured by propidium iodide fluorescence. CA, cornu ammonis; DG, dentate gyrus.

Mentions: In summary, compared to control group, in the 7 DIV group, tissues exposed to 10% O2 for 60 minutes tended to have decreased damage in the CA1 and DG (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900, DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant (Fig. 3).


Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures.

Kim SH, Lee WS, Lee NM, Chae SA, Yun SW - Korean J Pediatr (2015)

Percentage of cell death in the 7 days in vitro group, as measured by propidium iodide fluorescence. CA, cornu ammonis; DG, dentate gyrus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414629&req=5

Figure 3: Percentage of cell death in the 7 days in vitro group, as measured by propidium iodide fluorescence. CA, cornu ammonis; DG, dentate gyrus.
Mentions: In summary, compared to control group, in the 7 DIV group, tissues exposed to 10% O2 for 60 minutes tended to have decreased damage in the CA1 and DG (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900, DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant (Fig. 3).

Bottom Line: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant.In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.

Methods: We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.

Results: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).

Conclusion: Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.

No MeSH data available.


Related in: MedlinePlus