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Study of 27 Aqueous Humor Cytokines in Type 2 Diabetic Patients with or without Macular Edema.

Dong N, Xu B, Chu L, Tang X - PLoS ONE (2015)

Bottom Line: Compared with diabetic patients without ME, diabetic patients with ME had significantly higher concentrations of IL-1β, IL-6, IL-8, IP-10, MCP-1, and VEGF in the aqueous humor.However, the concentrations of IL-10 and IL-12 were significantly lower in the diabetic patients with ME.A variety of cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of diabetic macular edema, and both anti-inflammatory and antiangiogenic agents should be included in the treatment of ME simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
The aim of the present study was to compare the changes in the levels of 27 aqueous humor cytokines between diabetic patients with macular edema (ME) and diabetic patients without ME. Undiluted aqueous humor samples were obtained from 68 consecutive type 2 diabetic patients without ME and 56 consecutive type 2 diabetic patients with ME. The concentrations of 27 cytokines in the aqueous humor samples were measured using a multiplex bead immunoassay. Compared with diabetic patients without ME, diabetic patients with ME had significantly higher concentrations of IL-1β, IL-6, IL-8, IP-10, MCP-1, and VEGF in the aqueous humor. However, the concentrations of IL-10 and IL-12 were significantly lower in the diabetic patients with ME. The aqueous humor levels of IL-1β, IL-6, IL-8, MCP-1, IP-10, and VEGF were closely correlated with retinal macular thickness, retinal macular volume and the severity of ME. In addition, the aqueous humor levels of IL-10 and IL-12 decreased with increasing the severity of ME. A variety of cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of diabetic macular edema, and both anti-inflammatory and antiangiogenic agents should be included in the treatment of ME simultaneously.

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Related in: MedlinePlus

Scatterplots of the correlations between retinal macular thickness and the aqueous humour concentrations of cytokines.A. IL-1β. B. IL-6. C. IL-8. D. IP-10. E. MCP-1. F. VEGF. G. IL-10. H. IL-12.
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pone.0125329.g001: Scatterplots of the correlations between retinal macular thickness and the aqueous humour concentrations of cytokines.A. IL-1β. B. IL-6. C. IL-8. D. IP-10. E. MCP-1. F. VEGF. G. IL-10. H. IL-12.

Mentions: Table 4 and Fig 1 show the relationship between the concentrations of assayed cytokines and retinal macular thickness. The aqueous levels of IL-1β (r = 0.238), IL-6 (r = 0.477), IL-8 (r = 0.382), IP-10 (r = 0.386), MCP-1 (r = 0.504), and VEGF (r = 0.543) were found to positively correlate with retinal macular thickness. In addition, the aqueous level of IL-10 (r = -0.322) and IL-12 (r = -0.277) was negatively correlated with retinal macular thickness. Furthermore, the multiple regression analysis revealed independent influences of IL-1β (β = 0.107), IL-6 (β = 0.203), IL-8 (β = 0.156), IP-10 (β = 0.165), MCP-1 (β = 0.321), VEGF (β = 0.405), IL-10 (β = -0.135), and IL-12 (β = -0.048) on retinal macular thickness.


Study of 27 Aqueous Humor Cytokines in Type 2 Diabetic Patients with or without Macular Edema.

Dong N, Xu B, Chu L, Tang X - PLoS ONE (2015)

Scatterplots of the correlations between retinal macular thickness and the aqueous humour concentrations of cytokines.A. IL-1β. B. IL-6. C. IL-8. D. IP-10. E. MCP-1. F. VEGF. G. IL-10. H. IL-12.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414516&req=5

pone.0125329.g001: Scatterplots of the correlations between retinal macular thickness and the aqueous humour concentrations of cytokines.A. IL-1β. B. IL-6. C. IL-8. D. IP-10. E. MCP-1. F. VEGF. G. IL-10. H. IL-12.
Mentions: Table 4 and Fig 1 show the relationship between the concentrations of assayed cytokines and retinal macular thickness. The aqueous levels of IL-1β (r = 0.238), IL-6 (r = 0.477), IL-8 (r = 0.382), IP-10 (r = 0.386), MCP-1 (r = 0.504), and VEGF (r = 0.543) were found to positively correlate with retinal macular thickness. In addition, the aqueous level of IL-10 (r = -0.322) and IL-12 (r = -0.277) was negatively correlated with retinal macular thickness. Furthermore, the multiple regression analysis revealed independent influences of IL-1β (β = 0.107), IL-6 (β = 0.203), IL-8 (β = 0.156), IP-10 (β = 0.165), MCP-1 (β = 0.321), VEGF (β = 0.405), IL-10 (β = -0.135), and IL-12 (β = -0.048) on retinal macular thickness.

Bottom Line: Compared with diabetic patients without ME, diabetic patients with ME had significantly higher concentrations of IL-1β, IL-6, IL-8, IP-10, MCP-1, and VEGF in the aqueous humor.However, the concentrations of IL-10 and IL-12 were significantly lower in the diabetic patients with ME.A variety of cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of diabetic macular edema, and both anti-inflammatory and antiangiogenic agents should be included in the treatment of ME simultaneously.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
The aim of the present study was to compare the changes in the levels of 27 aqueous humor cytokines between diabetic patients with macular edema (ME) and diabetic patients without ME. Undiluted aqueous humor samples were obtained from 68 consecutive type 2 diabetic patients without ME and 56 consecutive type 2 diabetic patients with ME. The concentrations of 27 cytokines in the aqueous humor samples were measured using a multiplex bead immunoassay. Compared with diabetic patients without ME, diabetic patients with ME had significantly higher concentrations of IL-1β, IL-6, IL-8, IP-10, MCP-1, and VEGF in the aqueous humor. However, the concentrations of IL-10 and IL-12 were significantly lower in the diabetic patients with ME. The aqueous humor levels of IL-1β, IL-6, IL-8, MCP-1, IP-10, and VEGF were closely correlated with retinal macular thickness, retinal macular volume and the severity of ME. In addition, the aqueous humor levels of IL-10 and IL-12 decreased with increasing the severity of ME. A variety of cytokines associated with inflammation and angiogenesis may contribute to the pathogenesis of diabetic macular edema, and both anti-inflammatory and antiangiogenic agents should be included in the treatment of ME simultaneously.

Show MeSH
Related in: MedlinePlus