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Male Sexual Behavior and Pheromone Emission Is Enhanced by Exposure to Guava Fruit Volatiles in Anastrepha fraterculus.

Bachmann GE, Segura DF, Devescovi F, Juárez ML, Ruiz MJ, Vera MT, Cladera JL, Teal PE, Fernández PC - PLoS ONE (2015)

Bottom Line: Changes in male behavior appear to be particularly important during the initial phase of the sexual activity period, when most of the mating pairs are formed.This finding has important implications for the management of this pest species through the Sterile Insect Technique.We discuss the possibility of using artificial blends to improve the sexual competitiveness of sterile males.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Genética "E.A. Favret", Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.

ABSTRACT

Background: Plant chemicals can affect reproductive strategies of tephritid fruit flies by influencing sex pheromone communication and increasing male mating competitiveness.

Objective and methodology: We explored whether exposure of Anastrepha fraterculus males to guava fruit volatiles and to a synthetic blend of volatile compounds released by this fruit affects the sexual performance of wild and laboratory flies. By means of bioassays and pheromone collection we investigated the mechanism underlying this phenomenon.

Results: Guava volatile exposure enhanced male mating success and positively affected male calling behavior and pheromone release in laboratory and wild males. Changes in male behavior appear to be particularly important during the initial phase of the sexual activity period, when most of the mating pairs are formed. Exposure of laboratory males to a subset of guava fruit volatiles enhanced mating success, showing that the response to the fruit might be mimicked artificially.

Conclusions: Volatiles of guava seem to influence male mating success through an enhancement of chemical and physical signals related to the communication between sexes. This finding has important implications for the management of this pest species through the Sterile Insect Technique. We discuss the possibility of using artificial blends to improve the sexual competitiveness of sterile males.

No MeSH data available.


Related in: MedlinePlus

Effect of guava exposure on male calling behavior and pheromone release.a) Number of males that were detected fanning their wings across the observational period (mean ± SE) (mixed effect model P<0.001). b) Number of males that were detected exposing their salivary glands across the observational period (mean ± SE) (mixed effect model P = 0.076). c) Anastrephin released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.040). d) Epianastrephine released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.0104). e) Amount (ng) of (E,E)-α-farnesene released per male SE) (mixed effect model P = 0.083). f) Suspensolide released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.005). In all cases male origin factor and interaction were non-significant.
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pone.0124250.g002: Effect of guava exposure on male calling behavior and pheromone release.a) Number of males that were detected fanning their wings across the observational period (mean ± SE) (mixed effect model P<0.001). b) Number of males that were detected exposing their salivary glands across the observational period (mean ± SE) (mixed effect model P = 0.076). c) Anastrephin released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.040). d) Epianastrephine released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.0104). e) Amount (ng) of (E,E)-α-farnesene released per male SE) (mixed effect model P = 0.083). f) Suspensolide released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.005). In all cases male origin factor and interaction were non-significant.

Mentions: The mean number of males performing wing fanning was significantly higher in guava-exposed mated compared to non-exposed unmated males (F1,5 = 49, N = 6, P<0.001) (Fig 2A). The mean number of males exposing their salivary glands showed the same tendency, but differences between exposed and non-exposed males were non-significant (F1,5 = 5.0, N = 6, P = 0.076) (Fig 2B). The amount of (E,E) -α-farnesene, anastrephin, epianastrephin and suspensolide released was always higher for guava-exposed mated males (Fig 2C–2F). These differences were statistically significant for all compounds, except for (E,E) -α-farnesene (anastrephin: F1,6 = 6.80, N = 7, P = 0.040; epianastrephin: F1,6 = 13.53, N = 7, P = 0.010; (E,E)-α-farnesene: F1,6 = 4.33, N = 7, P = 0.083; suspensolide: F1,6 = 9.09, N = 7, P = 0.005). The impact of male origin was in all cases irrelevant (less than 1% of the total variance. Correlations among parameters related to the male calling behavior (i.e. wing fanning and salivary glands exposure) and individual pheromone compounds were all significant with P<0.05 except for wing fanning vs. anastrephin (P = 0.09, S1 Table).


Male Sexual Behavior and Pheromone Emission Is Enhanced by Exposure to Guava Fruit Volatiles in Anastrepha fraterculus.

Bachmann GE, Segura DF, Devescovi F, Juárez ML, Ruiz MJ, Vera MT, Cladera JL, Teal PE, Fernández PC - PLoS ONE (2015)

Effect of guava exposure on male calling behavior and pheromone release.a) Number of males that were detected fanning their wings across the observational period (mean ± SE) (mixed effect model P<0.001). b) Number of males that were detected exposing their salivary glands across the observational period (mean ± SE) (mixed effect model P = 0.076). c) Anastrephin released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.040). d) Epianastrephine released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.0104). e) Amount (ng) of (E,E)-α-farnesene released per male SE) (mixed effect model P = 0.083). f) Suspensolide released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.005). In all cases male origin factor and interaction were non-significant.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4414461&req=5

pone.0124250.g002: Effect of guava exposure on male calling behavior and pheromone release.a) Number of males that were detected fanning their wings across the observational period (mean ± SE) (mixed effect model P<0.001). b) Number of males that were detected exposing their salivary glands across the observational period (mean ± SE) (mixed effect model P = 0.076). c) Anastrephin released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.040). d) Epianastrephine released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.0104). e) Amount (ng) of (E,E)-α-farnesene released per male SE) (mixed effect model P = 0.083). f) Suspensolide released per male (mean proportion to (E,E)-α-farnesene ± SE) (mixed effect model P = 0.005). In all cases male origin factor and interaction were non-significant.
Mentions: The mean number of males performing wing fanning was significantly higher in guava-exposed mated compared to non-exposed unmated males (F1,5 = 49, N = 6, P<0.001) (Fig 2A). The mean number of males exposing their salivary glands showed the same tendency, but differences between exposed and non-exposed males were non-significant (F1,5 = 5.0, N = 6, P = 0.076) (Fig 2B). The amount of (E,E) -α-farnesene, anastrephin, epianastrephin and suspensolide released was always higher for guava-exposed mated males (Fig 2C–2F). These differences were statistically significant for all compounds, except for (E,E) -α-farnesene (anastrephin: F1,6 = 6.80, N = 7, P = 0.040; epianastrephin: F1,6 = 13.53, N = 7, P = 0.010; (E,E)-α-farnesene: F1,6 = 4.33, N = 7, P = 0.083; suspensolide: F1,6 = 9.09, N = 7, P = 0.005). The impact of male origin was in all cases irrelevant (less than 1% of the total variance. Correlations among parameters related to the male calling behavior (i.e. wing fanning and salivary glands exposure) and individual pheromone compounds were all significant with P<0.05 except for wing fanning vs. anastrephin (P = 0.09, S1 Table).

Bottom Line: Changes in male behavior appear to be particularly important during the initial phase of the sexual activity period, when most of the mating pairs are formed.This finding has important implications for the management of this pest species through the Sterile Insect Technique.We discuss the possibility of using artificial blends to improve the sexual competitiveness of sterile males.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Genética "E.A. Favret", Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.

ABSTRACT

Background: Plant chemicals can affect reproductive strategies of tephritid fruit flies by influencing sex pheromone communication and increasing male mating competitiveness.

Objective and methodology: We explored whether exposure of Anastrepha fraterculus males to guava fruit volatiles and to a synthetic blend of volatile compounds released by this fruit affects the sexual performance of wild and laboratory flies. By means of bioassays and pheromone collection we investigated the mechanism underlying this phenomenon.

Results: Guava volatile exposure enhanced male mating success and positively affected male calling behavior and pheromone release in laboratory and wild males. Changes in male behavior appear to be particularly important during the initial phase of the sexual activity period, when most of the mating pairs are formed. Exposure of laboratory males to a subset of guava fruit volatiles enhanced mating success, showing that the response to the fruit might be mimicked artificially.

Conclusions: Volatiles of guava seem to influence male mating success through an enhancement of chemical and physical signals related to the communication between sexes. This finding has important implications for the management of this pest species through the Sterile Insect Technique. We discuss the possibility of using artificial blends to improve the sexual competitiveness of sterile males.

No MeSH data available.


Related in: MedlinePlus