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Sequence Analysis of Insecticide Action and Detoxification-Related Genes in the Insect Pest Natural Enemy Pardosa pseudoannulata.

Meng X, Zhang Y, Bao H, Liu Z - PLoS ONE (2015)

Bottom Line: The pond wolf spider Pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields.The results differed from insects in which most P450 genes were in CYP3 and CYP4 clades.For GSTs, most unigenes belonged to the delta and sigma classes, and no epsilon GST class gene was found, which differed from the findings for insects and acarina.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The pond wolf spider Pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields. However, data on the genes involved in insecticide action, detoxification, and response are very limited for P. pseudoannulata, which inhibits the development and appropriate use of selective insecticides to control insect pests on rice. We used transcriptome construction from adult spider cephalothoraxes to analyze and manually identify genes enconding metabolic enzymes and target receptors related to insecticide action and detoxification, including 90 cytochrome P450s, 14 glutathione S-transferases (GSTs), 17 acetylcholinesterases (AChEs), 17 nicotinic acetylcholine receptors (nAChRs), and 17 gamma-aminobutyric acid (GABA) receptors, as well as 12 glutamate-gated chloride channel (GluCl) unigenes. Sequence alignment and phylogenetic analysis revealed the different subclassifications of P450s and GSTs, some important sequence diversities in nAChRs and GABA receptors, polymorphism in AChEs, and high similarities in GluCls. For P450s in P. pseudoannulata, the number of unigenes belonging to the CYP2 clade was much higher than that in CYP3 and CYP4 clades. The results differed from insects in which most P450 genes were in CYP3 and CYP4 clades. For GSTs, most unigenes belonged to the delta and sigma classes, and no epsilon GST class gene was found, which differed from the findings for insects and acarina. Our results will be useful for studies on insecticide action, selectivity, and detoxification in the spider and other related animals, and the sequence differences in target genes between the spider and insects will provide important information for the design of selective insecticides.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of GST unigenes in the P. pseudoannulata transcriptome compared with insect GSTs.Numbers above the branches indicate phylogenies from amino acid sequences and only values above 45% are shown. Dm: Drosophila melanogaster; Nl: Nilaparvata lugens; Sf: Sogatella furcifera.
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pone.0125242.g002: Phylogenetic analysis of GST unigenes in the P. pseudoannulata transcriptome compared with insect GSTs.Numbers above the branches indicate phylogenies from amino acid sequences and only values above 45% are shown. Dm: Drosophila melanogaster; Nl: Nilaparvata lugens; Sf: Sogatella furcifera.

Mentions: In the present study, 14 GST unigenes were found in the P. pseudoannulata transcriptome belonging to three classes: delta (6/14), sigma (5/14), microsomal (2/14), and one unknown (S4 Table). Phylogenetic analysis with model insects produced 12 GST unigenes (Fig 2), most of which belonged to the delta and sigma classes, and no epsilon GST gene was found. The results differed from those for insects, in which the main GST classes were delta and epsilon, with no microsomal class. Moreover, the findings were also different from the main GST classes in acarina, which were delta and microsomal classes, but without sigma class [34]. In the multiped spider P. pseudoannulata, which has no wings, the sigma class GSTs possibly mainly function in the muscles related to jumping, but not in flight muscles.


Sequence Analysis of Insecticide Action and Detoxification-Related Genes in the Insect Pest Natural Enemy Pardosa pseudoannulata.

Meng X, Zhang Y, Bao H, Liu Z - PLoS ONE (2015)

Phylogenetic analysis of GST unigenes in the P. pseudoannulata transcriptome compared with insect GSTs.Numbers above the branches indicate phylogenies from amino acid sequences and only values above 45% are shown. Dm: Drosophila melanogaster; Nl: Nilaparvata lugens; Sf: Sogatella furcifera.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414451&req=5

pone.0125242.g002: Phylogenetic analysis of GST unigenes in the P. pseudoannulata transcriptome compared with insect GSTs.Numbers above the branches indicate phylogenies from amino acid sequences and only values above 45% are shown. Dm: Drosophila melanogaster; Nl: Nilaparvata lugens; Sf: Sogatella furcifera.
Mentions: In the present study, 14 GST unigenes were found in the P. pseudoannulata transcriptome belonging to three classes: delta (6/14), sigma (5/14), microsomal (2/14), and one unknown (S4 Table). Phylogenetic analysis with model insects produced 12 GST unigenes (Fig 2), most of which belonged to the delta and sigma classes, and no epsilon GST gene was found. The results differed from those for insects, in which the main GST classes were delta and epsilon, with no microsomal class. Moreover, the findings were also different from the main GST classes in acarina, which were delta and microsomal classes, but without sigma class [34]. In the multiped spider P. pseudoannulata, which has no wings, the sigma class GSTs possibly mainly function in the muscles related to jumping, but not in flight muscles.

Bottom Line: The pond wolf spider Pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields.The results differed from insects in which most P450 genes were in CYP3 and CYP4 clades.For GSTs, most unigenes belonged to the delta and sigma classes, and no epsilon GST class gene was found, which differed from the findings for insects and acarina.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The pond wolf spider Pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields. However, data on the genes involved in insecticide action, detoxification, and response are very limited for P. pseudoannulata, which inhibits the development and appropriate use of selective insecticides to control insect pests on rice. We used transcriptome construction from adult spider cephalothoraxes to analyze and manually identify genes enconding metabolic enzymes and target receptors related to insecticide action and detoxification, including 90 cytochrome P450s, 14 glutathione S-transferases (GSTs), 17 acetylcholinesterases (AChEs), 17 nicotinic acetylcholine receptors (nAChRs), and 17 gamma-aminobutyric acid (GABA) receptors, as well as 12 glutamate-gated chloride channel (GluCl) unigenes. Sequence alignment and phylogenetic analysis revealed the different subclassifications of P450s and GSTs, some important sequence diversities in nAChRs and GABA receptors, polymorphism in AChEs, and high similarities in GluCls. For P450s in P. pseudoannulata, the number of unigenes belonging to the CYP2 clade was much higher than that in CYP3 and CYP4 clades. The results differed from insects in which most P450 genes were in CYP3 and CYP4 clades. For GSTs, most unigenes belonged to the delta and sigma classes, and no epsilon GST class gene was found, which differed from the findings for insects and acarina. Our results will be useful for studies on insecticide action, selectivity, and detoxification in the spider and other related animals, and the sequence differences in target genes between the spider and insects will provide important information for the design of selective insecticides.

No MeSH data available.


Related in: MedlinePlus