Limits...
Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats.

Gangwar M, Gautam MK, Ghildiyal S, Nath G, Goel RK - BMC Complement Altern Med (2015)

Bottom Line: MPE was found safe when given to rats upto 10 times of optimal effective dose.MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01).Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. mayankgangwar2008@gmail.com.

ABSTRACT

Background: Mallotus philippinensis Muell. Arg (MP, Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions. Since, Mallotus philippinensis have been shown to have a number of medicinal values. Hence our present study was to investigate the healing potential of fruit extract in rat wound models.

Methods: The study includes acute toxicity and wound healing potential of 50% ethanol extract of MP fruit glandular hair (MPE). MPE (200 mg/kg) was administered orally, once daily for 10 days (incision and dead space wound) and 22 days (excision wound). MPE was found safe when given to rats upto 10 times of optimal effective dose. Wound breaking strength (WBS) in Incision wound and rate of contraction, period of epithelization and scar area in Excision wound were evaluated. Granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants (catalase, superoxide dismutase, and reduced glutathione), acute inflammatory marker (myeloperoxidase), connective tissue markers (hydroxyproline, hexosamine, and hexuronic acid), and deep connective tissue histology were studied in Dead space wound.

Results: MPE significantly increased WBS and enhanced wound contraction, and decreased both epithelization period and scar area compared with control group. MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01). Histopathological evaluation revealed more density of collagen formation with minimal inflammatory cells in deeper tissues.

Conclusion: Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

Show MeSH

Related in: MedlinePlus

Histopathological examination of dead space granulation tissue at day 10 stained with H&E (100 x). (a) granulation tissue of control rat showed mononuclear inflammatory cells (black arrow), scattered abundance of eosinophilic fibroblasts (red arrow). (b) MPE treated showing large number of collagen tissue (fibrosis) and neovascularisation with minimal inflammatory cells. (c) VTE treated showing near to normal features, collagen tissue (fibrosis) and neovascularisation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4414308&req=5

Fig4: Histopathological examination of dead space granulation tissue at day 10 stained with H&E (100 x). (a) granulation tissue of control rat showed mononuclear inflammatory cells (black arrow), scattered abundance of eosinophilic fibroblasts (red arrow). (b) MPE treated showing large number of collagen tissue (fibrosis) and neovascularisation with minimal inflammatory cells. (c) VTE treated showing near to normal features, collagen tissue (fibrosis) and neovascularisation.

Mentions: Histology of deep granulation tissue of control rat showed scattered eosinophilic collagen tissue and some mononuclear inflammatory cells while, MPE and VTE treated rats showed large number of collagen tissue, neovascularisation with minimal mononuclear inflammatory cells (FigureĀ 4).Figure 4


Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats.

Gangwar M, Gautam MK, Ghildiyal S, Nath G, Goel RK - BMC Complement Altern Med (2015)

Histopathological examination of dead space granulation tissue at day 10 stained with H&E (100 x). (a) granulation tissue of control rat showed mononuclear inflammatory cells (black arrow), scattered abundance of eosinophilic fibroblasts (red arrow). (b) MPE treated showing large number of collagen tissue (fibrosis) and neovascularisation with minimal inflammatory cells. (c) VTE treated showing near to normal features, collagen tissue (fibrosis) and neovascularisation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4414308&req=5

Fig4: Histopathological examination of dead space granulation tissue at day 10 stained with H&E (100 x). (a) granulation tissue of control rat showed mononuclear inflammatory cells (black arrow), scattered abundance of eosinophilic fibroblasts (red arrow). (b) MPE treated showing large number of collagen tissue (fibrosis) and neovascularisation with minimal inflammatory cells. (c) VTE treated showing near to normal features, collagen tissue (fibrosis) and neovascularisation.
Mentions: Histology of deep granulation tissue of control rat showed scattered eosinophilic collagen tissue and some mononuclear inflammatory cells while, MPE and VTE treated rats showed large number of collagen tissue, neovascularisation with minimal mononuclear inflammatory cells (FigureĀ 4).Figure 4

Bottom Line: MPE was found safe when given to rats upto 10 times of optimal effective dose.MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01).Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. mayankgangwar2008@gmail.com.

ABSTRACT

Background: Mallotus philippinensis Muell. Arg (MP, Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions. Since, Mallotus philippinensis have been shown to have a number of medicinal values. Hence our present study was to investigate the healing potential of fruit extract in rat wound models.

Methods: The study includes acute toxicity and wound healing potential of 50% ethanol extract of MP fruit glandular hair (MPE). MPE (200 mg/kg) was administered orally, once daily for 10 days (incision and dead space wound) and 22 days (excision wound). MPE was found safe when given to rats upto 10 times of optimal effective dose. Wound breaking strength (WBS) in Incision wound and rate of contraction, period of epithelization and scar area in Excision wound were evaluated. Granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants (catalase, superoxide dismutase, and reduced glutathione), acute inflammatory marker (myeloperoxidase), connective tissue markers (hydroxyproline, hexosamine, and hexuronic acid), and deep connective tissue histology were studied in Dead space wound.

Results: MPE significantly increased WBS and enhanced wound contraction, and decreased both epithelization period and scar area compared with control group. MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01). Histopathological evaluation revealed more density of collagen formation with minimal inflammatory cells in deeper tissues.

Conclusion: Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

Show MeSH
Related in: MedlinePlus