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Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats.

Gangwar M, Gautam MK, Ghildiyal S, Nath G, Goel RK - BMC Complement Altern Med (2015)

Bottom Line: MPE was found safe when given to rats upto 10 times of optimal effective dose.MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01).Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. mayankgangwar2008@gmail.com.

ABSTRACT

Background: Mallotus philippinensis Muell. Arg (MP, Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions. Since, Mallotus philippinensis have been shown to have a number of medicinal values. Hence our present study was to investigate the healing potential of fruit extract in rat wound models.

Methods: The study includes acute toxicity and wound healing potential of 50% ethanol extract of MP fruit glandular hair (MPE). MPE (200 mg/kg) was administered orally, once daily for 10 days (incision and dead space wound) and 22 days (excision wound). MPE was found safe when given to rats upto 10 times of optimal effective dose. Wound breaking strength (WBS) in Incision wound and rate of contraction, period of epithelization and scar area in Excision wound were evaluated. Granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants (catalase, superoxide dismutase, and reduced glutathione), acute inflammatory marker (myeloperoxidase), connective tissue markers (hydroxyproline, hexosamine, and hexuronic acid), and deep connective tissue histology were studied in Dead space wound.

Results: MPE significantly increased WBS and enhanced wound contraction, and decreased both epithelization period and scar area compared with control group. MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01). Histopathological evaluation revealed more density of collagen formation with minimal inflammatory cells in deeper tissues.

Conclusion: Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

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Related in: MedlinePlus

Effect of MPE and VTE on deep granulation tissue antioxidants, reduced glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT). Results are mean ± SEM of 6 rats in each group. Control values of GSH, 13.1±0.56 nM/mg protein; SOD, 323.3±21.7 mU/mg protein and CAT, 37.0±3.75 mU/mg protein. P values: a< 0.05 and c< 0.001 compared to respective control group. (Statistical analysis was done by one way analysis of variance followed by Dunnett’s test for multiple comparisons).
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Fig2: Effect of MPE and VTE on deep granulation tissue antioxidants, reduced glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT). Results are mean ± SEM of 6 rats in each group. Control values of GSH, 13.1±0.56 nM/mg protein; SOD, 323.3±21.7 mU/mg protein and CAT, 37.0±3.75 mU/mg protein. P values: a< 0.05 and c< 0.001 compared to respective control group. (Statistical analysis was done by one way analysis of variance followed by Dunnett’s test for multiple comparisons).

Mentions: The mean control values for antioxidants, GSH, SOD and CAT were 13.1 ± 0.56 nM/mg protein, 323.3 ± 21.7 and 37.0 ± 3.75 mU/mg protein respectively; free radicals LPO and SOD were 5.57 ± 0.35 and 32.6 ± 1.15 nM/mg protein while, MPO was 30.2 ± 1.79 mU/mg protein. Both MPE and VTE showed increase in the level of antioxidants (P<0.001) while, free radicals and acute inflammatory marker, MPO were decreased (P<0.001) (Figures 2 and 3).Figure 2


Mallotus philippinensis Muell. Arg fruit glandular hairs extract promotes wound healing on different wound model in rats.

Gangwar M, Gautam MK, Ghildiyal S, Nath G, Goel RK - BMC Complement Altern Med (2015)

Effect of MPE and VTE on deep granulation tissue antioxidants, reduced glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT). Results are mean ± SEM of 6 rats in each group. Control values of GSH, 13.1±0.56 nM/mg protein; SOD, 323.3±21.7 mU/mg protein and CAT, 37.0±3.75 mU/mg protein. P values: a< 0.05 and c< 0.001 compared to respective control group. (Statistical analysis was done by one way analysis of variance followed by Dunnett’s test for multiple comparisons).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4414308&req=5

Fig2: Effect of MPE and VTE on deep granulation tissue antioxidants, reduced glutathione (GSH), Superoxide dismutase (SOD) and Catalase (CAT). Results are mean ± SEM of 6 rats in each group. Control values of GSH, 13.1±0.56 nM/mg protein; SOD, 323.3±21.7 mU/mg protein and CAT, 37.0±3.75 mU/mg protein. P values: a< 0.05 and c< 0.001 compared to respective control group. (Statistical analysis was done by one way analysis of variance followed by Dunnett’s test for multiple comparisons).
Mentions: The mean control values for antioxidants, GSH, SOD and CAT were 13.1 ± 0.56 nM/mg protein, 323.3 ± 21.7 and 37.0 ± 3.75 mU/mg protein respectively; free radicals LPO and SOD were 5.57 ± 0.35 and 32.6 ± 1.15 nM/mg protein while, MPO was 30.2 ± 1.79 mU/mg protein. Both MPE and VTE showed increase in the level of antioxidants (P<0.001) while, free radicals and acute inflammatory marker, MPO were decreased (P<0.001) (Figures 2 and 3).Figure 2

Bottom Line: MPE was found safe when given to rats upto 10 times of optimal effective dose.MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01).Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Modern Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. mayankgangwar2008@gmail.com.

ABSTRACT

Background: Mallotus philippinensis Muell. Arg (MP, Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions. Since, Mallotus philippinensis have been shown to have a number of medicinal values. Hence our present study was to investigate the healing potential of fruit extract in rat wound models.

Methods: The study includes acute toxicity and wound healing potential of 50% ethanol extract of MP fruit glandular hair (MPE). MPE (200 mg/kg) was administered orally, once daily for 10 days (incision and dead space wound) and 22 days (excision wound). MPE was found safe when given to rats upto 10 times of optimal effective dose. Wound breaking strength (WBS) in Incision wound and rate of contraction, period of epithelization and scar area in Excision wound were evaluated. Granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants (catalase, superoxide dismutase, and reduced glutathione), acute inflammatory marker (myeloperoxidase), connective tissue markers (hydroxyproline, hexosamine, and hexuronic acid), and deep connective tissue histology were studied in Dead space wound.

Results: MPE significantly increased WBS and enhanced wound contraction, and decreased both epithelization period and scar area compared with control group. MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01). Histopathological evaluation revealed more density of collagen formation with minimal inflammatory cells in deeper tissues.

Conclusion: Thus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.

Show MeSH
Related in: MedlinePlus