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Metabolic and pancreatic effects of bone marrow mesenchymal stem cells transplantation in mice fed high-fat diet.

Bueno Pde G, Yochite JN, Derigge-Pisani GF, Malmegrim de Farias KC, de Avó LR, Voltarelli JC, Leal ÂM - PLoS ONE (2015)

Bottom Line: Serum insulin concentration was higher in HFD-PBS group than in control animals and was not different compared with the other groups.The relative volume of α-cells was significantly smaller in HFD-PBS group than in C group and significantly higher in HFD-MSCs-NR than in HFD-PBS and HFD-MSCs-R groups.The results demonstrate the ability of multiple infusions of MSCs to promote prolonged decrease in hyperglycemia and apoptosis in pancreatic islets and increase in insulin sensitivity in HFD fed mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiological Science, Center of Biological Sciences and Health, Federal University of São Carlos, São Carlos, São Paulo, Brazil.

ABSTRACT
The purpose of this study was to investigate the effects of multiple infusions of allogeneic MSCs on glucose homeostasis and morphometry of pancreatic islets in high- fat diet (HFD) fed mice. Swiss mice were fed standard diet (C group) or HFD (HFD group). After 8 weeks, animals of HFD group received sterile phosphate-buffered saline infusions (HFD-PBS) or four infusions of MSCs one week apart (HFD-MSCs). Fasting glycemia (FG) was determined weekly and glucose (GTT) and insulin (ITT) tolerance tests were performed 4, 8, 12, and 16 weeks after the infusions of MSCs. The MSCs transplanted mice were classified as responder (FG < 180 mg/dL, 72.2% of transplanted mice) or non-responder (FG > 180mg/dL, 28.8%) Seven weeks after MSCs infusions, FG decreased in HFD-MSCs responder mice compared with the HFD-PBS group. Sixteen weeks post MSCs infusions, GTT and ITT areas under the curve (AUC) decreased in HFD-MSCs responder mice compared to HFD-PBS group. Serum insulin concentration was higher in HFD-PBS group than in control animals and was not different compared with the other groups. The relative volume of α-cells was significantly smaller in HFD-PBS group than in C group and significantly higher in HFD-MSCs-NR than in HFD-PBS and HFD-MSCs-R groups. Cell apoptosis in the islets was higher in HFD-PBS group than in C group, and lower in HFD-MSCs responder mice than in HFD-PBS group and non-responder animals. The results demonstrate the ability of multiple infusions of MSCs to promote prolonged decrease in hyperglycemia and apoptosis in pancreatic islets and increase in insulin sensitivity in HFD fed mice.

No MeSH data available.


Related in: MedlinePlus

Insulin Tolerance Test (ITT) glycemia response decrease post-MSCs infusion.Only in the 16th week post-MSC infusions, the glycemia response to insulin injection was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR compared to HFD-PBS group. Values are expressed as mean ± SEM (5–13 mice/group). * p < 0.05, control group (C) vs. HFD-PBS; # p < 0.05, HFD-MSCs-R vs. HFD-PBS; & p < 0.05, HFD-MSCs-NR vs. HFD-PBS.
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pone.0124369.g008: Insulin Tolerance Test (ITT) glycemia response decrease post-MSCs infusion.Only in the 16th week post-MSC infusions, the glycemia response to insulin injection was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR compared to HFD-PBS group. Values are expressed as mean ± SEM (5–13 mice/group). * p < 0.05, control group (C) vs. HFD-PBS; # p < 0.05, HFD-MSCs-R vs. HFD-PBS; & p < 0.05, HFD-MSCs-NR vs. HFD-PBS.

Mentions: Glycemia response to insulin injection (ITT) was significantly higher in HFD-PBS than in C at the fourth week post-MSCs or PBS infusions. Nevertheless, there was no difference among HFD-PBS, HFD-MSCs-R and HFD-MSCs-NR groups. The same pattern was observed in the 8th and 12th weeks post-MSCs or PBS infusions (data not shown). In the 16th week post-MSCs or PBS infusions, the glycemia response to insulin was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR than in HFD-PBS group (Fig 8).


Metabolic and pancreatic effects of bone marrow mesenchymal stem cells transplantation in mice fed high-fat diet.

Bueno Pde G, Yochite JN, Derigge-Pisani GF, Malmegrim de Farias KC, de Avó LR, Voltarelli JC, Leal ÂM - PLoS ONE (2015)

Insulin Tolerance Test (ITT) glycemia response decrease post-MSCs infusion.Only in the 16th week post-MSC infusions, the glycemia response to insulin injection was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR compared to HFD-PBS group. Values are expressed as mean ± SEM (5–13 mice/group). * p < 0.05, control group (C) vs. HFD-PBS; # p < 0.05, HFD-MSCs-R vs. HFD-PBS; & p < 0.05, HFD-MSCs-NR vs. HFD-PBS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414281&req=5

pone.0124369.g008: Insulin Tolerance Test (ITT) glycemia response decrease post-MSCs infusion.Only in the 16th week post-MSC infusions, the glycemia response to insulin injection was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR compared to HFD-PBS group. Values are expressed as mean ± SEM (5–13 mice/group). * p < 0.05, control group (C) vs. HFD-PBS; # p < 0.05, HFD-MSCs-R vs. HFD-PBS; & p < 0.05, HFD-MSCs-NR vs. HFD-PBS.
Mentions: Glycemia response to insulin injection (ITT) was significantly higher in HFD-PBS than in C at the fourth week post-MSCs or PBS infusions. Nevertheless, there was no difference among HFD-PBS, HFD-MSCs-R and HFD-MSCs-NR groups. The same pattern was observed in the 8th and 12th weeks post-MSCs or PBS infusions (data not shown). In the 16th week post-MSCs or PBS infusions, the glycemia response to insulin was significantly lower in both HFD-MSCs-R and HFD-MSCs-NR than in HFD-PBS group (Fig 8).

Bottom Line: Serum insulin concentration was higher in HFD-PBS group than in control animals and was not different compared with the other groups.The relative volume of α-cells was significantly smaller in HFD-PBS group than in C group and significantly higher in HFD-MSCs-NR than in HFD-PBS and HFD-MSCs-R groups.The results demonstrate the ability of multiple infusions of MSCs to promote prolonged decrease in hyperglycemia and apoptosis in pancreatic islets and increase in insulin sensitivity in HFD fed mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiological Science, Center of Biological Sciences and Health, Federal University of São Carlos, São Carlos, São Paulo, Brazil.

ABSTRACT
The purpose of this study was to investigate the effects of multiple infusions of allogeneic MSCs on glucose homeostasis and morphometry of pancreatic islets in high- fat diet (HFD) fed mice. Swiss mice were fed standard diet (C group) or HFD (HFD group). After 8 weeks, animals of HFD group received sterile phosphate-buffered saline infusions (HFD-PBS) or four infusions of MSCs one week apart (HFD-MSCs). Fasting glycemia (FG) was determined weekly and glucose (GTT) and insulin (ITT) tolerance tests were performed 4, 8, 12, and 16 weeks after the infusions of MSCs. The MSCs transplanted mice were classified as responder (FG < 180 mg/dL, 72.2% of transplanted mice) or non-responder (FG > 180mg/dL, 28.8%) Seven weeks after MSCs infusions, FG decreased in HFD-MSCs responder mice compared with the HFD-PBS group. Sixteen weeks post MSCs infusions, GTT and ITT areas under the curve (AUC) decreased in HFD-MSCs responder mice compared to HFD-PBS group. Serum insulin concentration was higher in HFD-PBS group than in control animals and was not different compared with the other groups. The relative volume of α-cells was significantly smaller in HFD-PBS group than in C group and significantly higher in HFD-MSCs-NR than in HFD-PBS and HFD-MSCs-R groups. Cell apoptosis in the islets was higher in HFD-PBS group than in C group, and lower in HFD-MSCs responder mice than in HFD-PBS group and non-responder animals. The results demonstrate the ability of multiple infusions of MSCs to promote prolonged decrease in hyperglycemia and apoptosis in pancreatic islets and increase in insulin sensitivity in HFD fed mice.

No MeSH data available.


Related in: MedlinePlus