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MicroRNA-302a Suppresses Tumor Cell Proliferation by Inhibiting AKT in Prostate Cancer.

Zhang GM, Bao CY, Wan FN, Cao DL, Qin XJ, Zhang HL, Zhu Y, Dai B, Shi GH, Ye DW - PLoS ONE (2015)

Bottom Line: Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a.Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo.Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

ABSTRACT
Micro (mi) RNAs are important regulators involved in various physical and pathological processes, including cancer. The miRNA-302 family has been documented as playing a critical role in carcinogenesis. In this study, we investigated the role of miRNA-302a in prostate cancer (PCa). MiRNA-302a expression was detected in 44 PCa tissues and 10 normal prostate tissues, and their clinicopathological significance was analyzed. Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a. The target gene of miRNA-302a and the downstream pathway were further investigated. Compared with normal prostate tissues, miRNA-302a expression was downregulated in PCa tissues, and was even lower in PCa tissues with a Gleason score ≥8. Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo. Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway. These results reveal miRNA-302a as a tumor suppressor in PCa, suggesting that miRNA-302a may be used as a potential target for therapeutic intervention in PCa.

No MeSH data available.


Related in: MedlinePlus

Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vivo.PC-3-GFP cells and PC-3-302a cells were injected into the left and right posterior flank of BALB/c nude mice, respectively (A, B). The tumor volume (C) and mass (D) in the PC-3-302a group were notably lower than in the PC-3-GFP group. (* P<0.05).
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pone.0124410.g003: Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vivo.PC-3-GFP cells and PC-3-302a cells were injected into the left and right posterior flank of BALB/c nude mice, respectively (A, B). The tumor volume (C) and mass (D) in the PC-3-302a group were notably lower than in the PC-3-GFP group. (* P<0.05).

Mentions: To further validate our observations in vivo, PC-3-Scr cells and PC-3-302a cells were injected into the left and right posterior flank of five nude mice, respectively. Tumor volumes were measured using calipers at different time points after inoculation, and tumor weights were measured after sacrifice. Both volume and mass were notably lower in PC-3-302a tumors than in PC-3-Scr tumors (P < 0.05; Fig 3). Taken together, obvious cell proliferation inhibition was observed after overexpression of miRNA-302a in PCa cells.


MicroRNA-302a Suppresses Tumor Cell Proliferation by Inhibiting AKT in Prostate Cancer.

Zhang GM, Bao CY, Wan FN, Cao DL, Qin XJ, Zhang HL, Zhu Y, Dai B, Shi GH, Ye DW - PLoS ONE (2015)

Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vivo.PC-3-GFP cells and PC-3-302a cells were injected into the left and right posterior flank of BALB/c nude mice, respectively (A, B). The tumor volume (C) and mass (D) in the PC-3-302a group were notably lower than in the PC-3-GFP group. (* P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414271&req=5

pone.0124410.g003: Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vivo.PC-3-GFP cells and PC-3-302a cells were injected into the left and right posterior flank of BALB/c nude mice, respectively (A, B). The tumor volume (C) and mass (D) in the PC-3-302a group were notably lower than in the PC-3-GFP group. (* P<0.05).
Mentions: To further validate our observations in vivo, PC-3-Scr cells and PC-3-302a cells were injected into the left and right posterior flank of five nude mice, respectively. Tumor volumes were measured using calipers at different time points after inoculation, and tumor weights were measured after sacrifice. Both volume and mass were notably lower in PC-3-302a tumors than in PC-3-Scr tumors (P < 0.05; Fig 3). Taken together, obvious cell proliferation inhibition was observed after overexpression of miRNA-302a in PCa cells.

Bottom Line: Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a.Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo.Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

ABSTRACT
Micro (mi) RNAs are important regulators involved in various physical and pathological processes, including cancer. The miRNA-302 family has been documented as playing a critical role in carcinogenesis. In this study, we investigated the role of miRNA-302a in prostate cancer (PCa). MiRNA-302a expression was detected in 44 PCa tissues and 10 normal prostate tissues, and their clinicopathological significance was analyzed. Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a. The target gene of miRNA-302a and the downstream pathway were further investigated. Compared with normal prostate tissues, miRNA-302a expression was downregulated in PCa tissues, and was even lower in PCa tissues with a Gleason score ≥8. Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo. Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway. These results reveal miRNA-302a as a tumor suppressor in PCa, suggesting that miRNA-302a may be used as a potential target for therapeutic intervention in PCa.

No MeSH data available.


Related in: MedlinePlus