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MicroRNA-302a Suppresses Tumor Cell Proliferation by Inhibiting AKT in Prostate Cancer.

Zhang GM, Bao CY, Wan FN, Cao DL, Qin XJ, Zhang HL, Zhu Y, Dai B, Shi GH, Ye DW - PLoS ONE (2015)

Bottom Line: Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a.Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo.Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

ABSTRACT
Micro (mi) RNAs are important regulators involved in various physical and pathological processes, including cancer. The miRNA-302 family has been documented as playing a critical role in carcinogenesis. In this study, we investigated the role of miRNA-302a in prostate cancer (PCa). MiRNA-302a expression was detected in 44 PCa tissues and 10 normal prostate tissues, and their clinicopathological significance was analyzed. Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a. The target gene of miRNA-302a and the downstream pathway were further investigated. Compared with normal prostate tissues, miRNA-302a expression was downregulated in PCa tissues, and was even lower in PCa tissues with a Gleason score ≥8. Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo. Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway. These results reveal miRNA-302a as a tumor suppressor in PCa, suggesting that miRNA-302a may be used as a potential target for therapeutic intervention in PCa.

No MeSH data available.


Related in: MedlinePlus

Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vitro.A CCK-8 assay was performed to measure proliferation in (A) PC-3 and (B) DU145 cells. Data represent the mean ± standard deviation of the optical density (OD) value detected at 450 nm from three independent experiments. Cell proliferation was detected in (C) PC-3 and (D) DU145 cells using EdU assay analyzed by flow cytometry. (E, F) Colony formation assays indicated fewer colonies in miRNA-302a overexpressing PCa cells. (*P<0.05).
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pone.0124410.g002: Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vitro.A CCK-8 assay was performed to measure proliferation in (A) PC-3 and (B) DU145 cells. Data represent the mean ± standard deviation of the optical density (OD) value detected at 450 nm from three independent experiments. Cell proliferation was detected in (C) PC-3 and (D) DU145 cells using EdU assay analyzed by flow cytometry. (E, F) Colony formation assays indicated fewer colonies in miRNA-302a overexpressing PCa cells. (*P<0.05).

Mentions: The CCK-8, EdU, and colony forming assays were carried out to examine whether miRNA-302a overexpression affected PCa cell proliferation in vitro. As shown in Fig 2, there was a significantly lower (P < 0.05) growth rate in PC-3-302a and DU145-302a cells compared with the controls. Flow cytometric analyses indicated that the percentages of EdU-positive cells in both PC-3-302a and DU145-302a cells were lower than in the controls. In addition, compared with the controls, both PC-3-302a and DU145-302a cells developed fewer colonies on the 20th and 15th days, respectively. Therefore, in vitro experiments demonstrate that miRNA-302a exerted a suppressive role in PCa cell proliferation.


MicroRNA-302a Suppresses Tumor Cell Proliferation by Inhibiting AKT in Prostate Cancer.

Zhang GM, Bao CY, Wan FN, Cao DL, Qin XJ, Zhang HL, Zhu Y, Dai B, Shi GH, Ye DW - PLoS ONE (2015)

Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vitro.A CCK-8 assay was performed to measure proliferation in (A) PC-3 and (B) DU145 cells. Data represent the mean ± standard deviation of the optical density (OD) value detected at 450 nm from three independent experiments. Cell proliferation was detected in (C) PC-3 and (D) DU145 cells using EdU assay analyzed by flow cytometry. (E, F) Colony formation assays indicated fewer colonies in miRNA-302a overexpressing PCa cells. (*P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4414271&req=5

pone.0124410.g002: Overexpression of miRNA-302a significantly inhibits cell proliferation in PCa cells in vitro.A CCK-8 assay was performed to measure proliferation in (A) PC-3 and (B) DU145 cells. Data represent the mean ± standard deviation of the optical density (OD) value detected at 450 nm from three independent experiments. Cell proliferation was detected in (C) PC-3 and (D) DU145 cells using EdU assay analyzed by flow cytometry. (E, F) Colony formation assays indicated fewer colonies in miRNA-302a overexpressing PCa cells. (*P<0.05).
Mentions: The CCK-8, EdU, and colony forming assays were carried out to examine whether miRNA-302a overexpression affected PCa cell proliferation in vitro. As shown in Fig 2, there was a significantly lower (P < 0.05) growth rate in PC-3-302a and DU145-302a cells compared with the controls. Flow cytometric analyses indicated that the percentages of EdU-positive cells in both PC-3-302a and DU145-302a cells were lower than in the controls. In addition, compared with the controls, both PC-3-302a and DU145-302a cells developed fewer colonies on the 20th and 15th days, respectively. Therefore, in vitro experiments demonstrate that miRNA-302a exerted a suppressive role in PCa cell proliferation.

Bottom Line: Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a.Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo.Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

ABSTRACT
Micro (mi) RNAs are important regulators involved in various physical and pathological processes, including cancer. The miRNA-302 family has been documented as playing a critical role in carcinogenesis. In this study, we investigated the role of miRNA-302a in prostate cancer (PCa). MiRNA-302a expression was detected in 44 PCa tissues and 10 normal prostate tissues, and their clinicopathological significance was analyzed. Cell proliferation and cell cycle analysis were performed on PCa cells that stably expressed miRNA-302a. The target gene of miRNA-302a and the downstream pathway were further investigated. Compared with normal prostate tissues, miRNA-302a expression was downregulated in PCa tissues, and was even lower in PCa tissues with a Gleason score ≥8. Overexpression of miRNA-302a induced G1/S cell cycle arrest in PCa cells, and suppressed PCa cell proliferation both in vitro and in vivo. Furthermore, miRNA-302a inhibits AKT expression by directly binding to its 3΄ untranslated region, resulting in subsequent alterations of the AKT-GSK3β-cyclin D1 and AKT-p27Kip1 pathway. These results reveal miRNA-302a as a tumor suppressor in PCa, suggesting that miRNA-302a may be used as a potential target for therapeutic intervention in PCa.

No MeSH data available.


Related in: MedlinePlus