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Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart.

Bialy D, Wawrzynska M, Bil-Lula I, Krzywonos-Zawadzka A, Wozniak M, Cadete VJ, Sawicki G - Biomed Res Int (2015)

Bottom Line: LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow.In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF.The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF.

View Article: PubMed Central - PubMed

Affiliation: Department and Clinic of Cardiology, Medical University of Wroclaw, 50-556 Wroclaw, Poland.

ABSTRACT
Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of the perfusion protocol for isolated rat hearts subjected to global no-flow ischemia and protected with low frequency electromagnetic field (LF-EMF).
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fig2: Schematic representation of the perfusion protocol for isolated rat hearts subjected to global no-flow ischemia and protected with low frequency electromagnetic field (LF-EMF).

Mentions: The scheme of the experimental protocol is shown in Figure 2. After 20 min of aerobic perfusions (control hearts) or at the first minutes of reperfusion (from I/R hearts) samples of perfusates were collected for measurement of interleukin-6 (IL-6) and tropoponin I (TnI). At the end of perfusion the hearts were freeze-clamped in liquid nitrogen and used for measurement of activity of matrix metalloproteinase-2 (MMP-2).


Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart.

Bialy D, Wawrzynska M, Bil-Lula I, Krzywonos-Zawadzka A, Wozniak M, Cadete VJ, Sawicki G - Biomed Res Int (2015)

Schematic representation of the perfusion protocol for isolated rat hearts subjected to global no-flow ischemia and protected with low frequency electromagnetic field (LF-EMF).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4414265&req=5

fig2: Schematic representation of the perfusion protocol for isolated rat hearts subjected to global no-flow ischemia and protected with low frequency electromagnetic field (LF-EMF).
Mentions: The scheme of the experimental protocol is shown in Figure 2. After 20 min of aerobic perfusions (control hearts) or at the first minutes of reperfusion (from I/R hearts) samples of perfusates were collected for measurement of interleukin-6 (IL-6) and tropoponin I (TnI). At the end of perfusion the hearts were freeze-clamped in liquid nitrogen and used for measurement of activity of matrix metalloproteinase-2 (MMP-2).

Bottom Line: LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow.In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF.The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF.

View Article: PubMed Central - PubMed

Affiliation: Department and Clinic of Cardiology, Medical University of Wroclaw, 50-556 Wroclaw, Poland.

ABSTRACT
Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.

No MeSH data available.


Related in: MedlinePlus