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Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart.

Bialy D, Wawrzynska M, Bil-Lula I, Krzywonos-Zawadzka A, Wozniak M, Cadete VJ, Sawicki G - Biomed Res Int (2015)

Bottom Line: LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow.In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF.The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF.

View Article: PubMed Central - PubMed

Affiliation: Department and Clinic of Cardiology, Medical University of Wroclaw, 50-556 Wroclaw, Poland.

ABSTRACT
Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.

No MeSH data available.


Related in: MedlinePlus

Diagram of dependence of the electromagnetic field to distance (d1–4) from the head of applicator to the isolated heart. Different electromagnetic fields are represented by B1–4. The arrow indicates increased distance and decreased electromagnetic field from the head. The estimated values of electromagnetic induction (B) in relation to distance (d) from the head of the applicator are showed in Table 1.
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fig1: Diagram of dependence of the electromagnetic field to distance (d1–4) from the head of applicator to the isolated heart. Different electromagnetic fields are represented by B1–4. The arrow indicates increased distance and decreased electromagnetic field from the head. The estimated values of electromagnetic induction (B) in relation to distance (d) from the head of the applicator are showed in Table 1.

Mentions: Low frequency electromagnetic fields (LF-EMF) were applied to a subset of hearts perfused either aerobically (n = 9) or subjected to I/R (n = 9). LF-EMF was generated by a point applicator Z connected to a Viofor JPS classic control unit (Viofor JPS, Poland). Magnetic field induction (B) varied, depending on the distance (d), from the inducing point applicator Z and averaged 500 μT. Figure 1 schematizes the dependence of the field on distance. Table 1 summarizes the field variations (B1–4) with respect to distance (d1–4).


Low Frequency Electromagnetic Field Conditioning Protects against I/R Injury and Contractile Dysfunction in the Isolated Rat Heart.

Bialy D, Wawrzynska M, Bil-Lula I, Krzywonos-Zawadzka A, Wozniak M, Cadete VJ, Sawicki G - Biomed Res Int (2015)

Diagram of dependence of the electromagnetic field to distance (d1–4) from the head of applicator to the isolated heart. Different electromagnetic fields are represented by B1–4. The arrow indicates increased distance and decreased electromagnetic field from the head. The estimated values of electromagnetic induction (B) in relation to distance (d) from the head of the applicator are showed in Table 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4414265&req=5

fig1: Diagram of dependence of the electromagnetic field to distance (d1–4) from the head of applicator to the isolated heart. Different electromagnetic fields are represented by B1–4. The arrow indicates increased distance and decreased electromagnetic field from the head. The estimated values of electromagnetic induction (B) in relation to distance (d) from the head of the applicator are showed in Table 1.
Mentions: Low frequency electromagnetic fields (LF-EMF) were applied to a subset of hearts perfused either aerobically (n = 9) or subjected to I/R (n = 9). LF-EMF was generated by a point applicator Z connected to a Viofor JPS classic control unit (Viofor JPS, Poland). Magnetic field induction (B) varied, depending on the distance (d), from the inducing point applicator Z and averaged 500 μT. Figure 1 schematizes the dependence of the field on distance. Table 1 summarizes the field variations (B1–4) with respect to distance (d1–4).

Bottom Line: LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow.In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF.The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF.

View Article: PubMed Central - PubMed

Affiliation: Department and Clinic of Cardiology, Medical University of Wroclaw, 50-556 Wroclaw, Poland.

ABSTRACT
Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.

No MeSH data available.


Related in: MedlinePlus