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Concurrent gastric and pulmonary mucosa-associated lymphoid tissue lymphomas with pre-existing intrinsic chronic inflammation: a case report and a review of the literature.

Oh SY, Kim NY, Oh DH, Bang SM, Choi YJ, Lee JY, Lee KW, Yoon HI, Yang HC, Paik JH, Lee DH, Jung HC - Gut Liver (2015)

View Article: PubMed Central - PubMed

ABSTRACT

Herein, we report a rare case of concurrent gastric and pulmonary mucosa-associated lymphoid tissue (MALT) lymphomas. A 65-year-old man who had been diagnosed with Helicobacter pylori-positive gastric MALT lymphoma received eradication therapy and achieved complete remission. During follow-up, he developed de novo pulmonary MALT lymphoma as a sequela of pulmonary tuberculosis, accompanied by recurrent gastric MALT lymphoma. Polymerase chain reaction (PCR) products of the CDR3 region of the immunoglobulin heavy chain gene showed an overall polyclonal pattern with bands at 400 base pairs (bp) and 200 bp predominant in the pulmonary tissue, as well as two distinctive bands in the gastric tissue at 400 bp and 200 bp. This case suggests that multiorgan lymphomas are more likely to be independent from each other when they are far apart, involve different organ systems, and have independent precipitating factors.

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Polymerase chain reaction (PCR) of the CDR3 region of immunoglobulin heavy chain gene was performed with formalin-fixed paraffin-embedded tissue samples. Electrophoresis of the PCR products demonstrated an overall polyclonal pattern with predominant bands at 400 base pairs (bp) and 200 bp in the pulmonary tissues (A), while two distinctive bands at 400 bp and 200 bp were noted in the gastric tissues (B).
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f4-gnl-09-424: Polymerase chain reaction (PCR) of the CDR3 region of immunoglobulin heavy chain gene was performed with formalin-fixed paraffin-embedded tissue samples. Electrophoresis of the PCR products demonstrated an overall polyclonal pattern with predominant bands at 400 base pairs (bp) and 200 bp in the pulmonary tissues (A), while two distinctive bands at 400 bp and 200 bp were noted in the gastric tissues (B).

Mentions: The lymphomas were believed to be stage IE gastric MALT lymphoma combined with stage IIE pulmonary MALT lymphoma or stage IV gastric MALT lymphoma with pulmonary dissemination. The absence of lymph node or bone marrow involvement seemed to indicate the former. For further investigation, PCR of the CDR3 region of IgH was performed with formalin-fixed paraffin-embedded tissue samples, according to previously published methods.2,3 The following consensus primers, which were specific for CDR3, were used: the first-round primer was LJH 5′-TGAGGAGACGGTGACC-3′; and the nested second-round primers were VLJH 5′-GTGACCAGGGTNCCTTG-GCCCCAG-3′, and FR3A 5′-ACACGGCSSTGTATTACTGT-3′. Electrophoresis of the PCR products showed an overall polyclonal pattern in the pulmonary tissues, even though bands at 400 base pairs (bp) and 200 bp predominated. Two distinctive bands at 400 bp and 200 bp were observed in the gastric tissues (Fig. 4).


Concurrent gastric and pulmonary mucosa-associated lymphoid tissue lymphomas with pre-existing intrinsic chronic inflammation: a case report and a review of the literature.

Oh SY, Kim NY, Oh DH, Bang SM, Choi YJ, Lee JY, Lee KW, Yoon HI, Yang HC, Paik JH, Lee DH, Jung HC - Gut Liver (2015)

Polymerase chain reaction (PCR) of the CDR3 region of immunoglobulin heavy chain gene was performed with formalin-fixed paraffin-embedded tissue samples. Electrophoresis of the PCR products demonstrated an overall polyclonal pattern with predominant bands at 400 base pairs (bp) and 200 bp in the pulmonary tissues (A), while two distinctive bands at 400 bp and 200 bp were noted in the gastric tissues (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4413978&req=5

f4-gnl-09-424: Polymerase chain reaction (PCR) of the CDR3 region of immunoglobulin heavy chain gene was performed with formalin-fixed paraffin-embedded tissue samples. Electrophoresis of the PCR products demonstrated an overall polyclonal pattern with predominant bands at 400 base pairs (bp) and 200 bp in the pulmonary tissues (A), while two distinctive bands at 400 bp and 200 bp were noted in the gastric tissues (B).
Mentions: The lymphomas were believed to be stage IE gastric MALT lymphoma combined with stage IIE pulmonary MALT lymphoma or stage IV gastric MALT lymphoma with pulmonary dissemination. The absence of lymph node or bone marrow involvement seemed to indicate the former. For further investigation, PCR of the CDR3 region of IgH was performed with formalin-fixed paraffin-embedded tissue samples, according to previously published methods.2,3 The following consensus primers, which were specific for CDR3, were used: the first-round primer was LJH 5′-TGAGGAGACGGTGACC-3′; and the nested second-round primers were VLJH 5′-GTGACCAGGGTNCCTTG-GCCCCAG-3′, and FR3A 5′-ACACGGCSSTGTATTACTGT-3′. Electrophoresis of the PCR products showed an overall polyclonal pattern in the pulmonary tissues, even though bands at 400 base pairs (bp) and 200 bp predominated. Two distinctive bands at 400 bp and 200 bp were observed in the gastric tissues (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

Herein, we report a rare case of concurrent gastric and pulmonary mucosa-associated lymphoid tissue (MALT) lymphomas. A 65-year-old man who had been diagnosed with Helicobacter pylori-positive gastric MALT lymphoma received eradication therapy and achieved complete remission. During follow-up, he developed de novo pulmonary MALT lymphoma as a sequela of pulmonary tuberculosis, accompanied by recurrent gastric MALT lymphoma. Polymerase chain reaction (PCR) products of the CDR3 region of the immunoglobulin heavy chain gene showed an overall polyclonal pattern with bands at 400 base pairs (bp) and 200 bp predominant in the pulmonary tissue, as well as two distinctive bands in the gastric tissue at 400 bp and 200 bp. This case suggests that multiorgan lymphomas are more likely to be independent from each other when they are far apart, involve different organ systems, and have independent precipitating factors.

Show MeSH