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Cytokine expression of microscopic colitis including interleukin-17.

Park EK, Park YS, Park DR, Jung SA, Han DS, Jang BI, Kim YH, Kim WH, Jo YJ, Lee KH, Lee WM, Kim EK, Koo HS - Gut Liver (2015)

Bottom Line: Cytokine expression was similar between collagenous colitis and lymphocytic colitis.However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis.The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Eulji University School of Medicine, Korea.

ABSTRACT

Background/aims: Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis.

Methods: This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six patients with functional diarrhea but normal pathology. We performed an immunohistochemical analysis of the colonic mucosal biopsies to assess the expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, interferon-γ, inducible nitric oxide synthase, and tumor necrosis factor-α. We compared the quantity score of immunohistochemical staining among the groups.

Results: The microscopic colitis group showed significantly higher expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, and interferon-γ compared with the control group. Cytokine expression was similar between collagenous colitis and lymphocytic colitis. However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis.

Conclusions: Proinflammatory cytokines, including interleukin-17 and interferon-γ, are highly expressed in microscopic colitis. The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis. This study is the first on interleukin-17 expression in microscopic colitis patients.

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Related in: MedlinePlus

Immunohistochemical staining results for interleukin-17 (IL-17) (×400). Immunohistochemical analysis comparing levels of IL-17 in control (A), lymphocytic colitis (LC) (B), and collagenous colitis (CC) (C). The positive expression of IL-17 is visualized as brown granules deposited in the cytoplasm of the epithelial and inflammatory cells. The arrow indicates intraepithelial lymphocytosis in LC (B). The arrowhead shows the subepithelial collagen layer in CC (C). The graph shows the average quantity score of each group (D). The microscopic colitis group showed an increased quantity score compared with the control group (p=0.001). There were no significant differences between the LC and CC groups (p=0.727) (D).
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f2-gnl-09-381: Immunohistochemical staining results for interleukin-17 (IL-17) (×400). Immunohistochemical analysis comparing levels of IL-17 in control (A), lymphocytic colitis (LC) (B), and collagenous colitis (CC) (C). The positive expression of IL-17 is visualized as brown granules deposited in the cytoplasm of the epithelial and inflammatory cells. The arrow indicates intraepithelial lymphocytosis in LC (B). The arrowhead shows the subepithelial collagen layer in CC (C). The graph shows the average quantity score of each group (D). The microscopic colitis group showed an increased quantity score compared with the control group (p=0.001). There were no significant differences between the LC and CC groups (p=0.727) (D).

Mentions: For IL-17, the epithelial and inflammatory cell scores were 1 and 0 in the control group, respectively (Fig. 2A). In the LC group, both the epithelial and inflammatory cell scores were 1, for a total score of 2 (Fig. 2B). In the CC group, the epithelial cell score was 2, and the inflammatory cell score was 1, for total score of 3 (Fig. 2C). There was a statistically significant difference between control and MC groups (p=0.001) (Fig. 2D).


Cytokine expression of microscopic colitis including interleukin-17.

Park EK, Park YS, Park DR, Jung SA, Han DS, Jang BI, Kim YH, Kim WH, Jo YJ, Lee KH, Lee WM, Kim EK, Koo HS - Gut Liver (2015)

Immunohistochemical staining results for interleukin-17 (IL-17) (×400). Immunohistochemical analysis comparing levels of IL-17 in control (A), lymphocytic colitis (LC) (B), and collagenous colitis (CC) (C). The positive expression of IL-17 is visualized as brown granules deposited in the cytoplasm of the epithelial and inflammatory cells. The arrow indicates intraepithelial lymphocytosis in LC (B). The arrowhead shows the subepithelial collagen layer in CC (C). The graph shows the average quantity score of each group (D). The microscopic colitis group showed an increased quantity score compared with the control group (p=0.001). There were no significant differences between the LC and CC groups (p=0.727) (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4413972&req=5

f2-gnl-09-381: Immunohistochemical staining results for interleukin-17 (IL-17) (×400). Immunohistochemical analysis comparing levels of IL-17 in control (A), lymphocytic colitis (LC) (B), and collagenous colitis (CC) (C). The positive expression of IL-17 is visualized as brown granules deposited in the cytoplasm of the epithelial and inflammatory cells. The arrow indicates intraepithelial lymphocytosis in LC (B). The arrowhead shows the subepithelial collagen layer in CC (C). The graph shows the average quantity score of each group (D). The microscopic colitis group showed an increased quantity score compared with the control group (p=0.001). There were no significant differences between the LC and CC groups (p=0.727) (D).
Mentions: For IL-17, the epithelial and inflammatory cell scores were 1 and 0 in the control group, respectively (Fig. 2A). In the LC group, both the epithelial and inflammatory cell scores were 1, for a total score of 2 (Fig. 2B). In the CC group, the epithelial cell score was 2, and the inflammatory cell score was 1, for total score of 3 (Fig. 2C). There was a statistically significant difference between control and MC groups (p=0.001) (Fig. 2D).

Bottom Line: Cytokine expression was similar between collagenous colitis and lymphocytic colitis.However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis.The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Eulji University School of Medicine, Korea.

ABSTRACT

Background/aims: Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis.

Methods: This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six patients with functional diarrhea but normal pathology. We performed an immunohistochemical analysis of the colonic mucosal biopsies to assess the expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, interferon-γ, inducible nitric oxide synthase, and tumor necrosis factor-α. We compared the quantity score of immunohistochemical staining among the groups.

Results: The microscopic colitis group showed significantly higher expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, and interferon-γ compared with the control group. Cytokine expression was similar between collagenous colitis and lymphocytic colitis. However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis.

Conclusions: Proinflammatory cytokines, including interleukin-17 and interferon-γ, are highly expressed in microscopic colitis. The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis. This study is the first on interleukin-17 expression in microscopic colitis patients.

Show MeSH
Related in: MedlinePlus