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Decrease in circulating dendritic cell precursors in patients with peripheral artery disease.

Kretzschmar D, Rohm I, Schäller S, Betge S, Pistulli R, Atiskova Y, Figulla HR, Yilmaz A - Mediators Inflamm. (2015)

Bottom Line: In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed.We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD.Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany.

ABSTRACT
Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n = 52) compared to controls (n = 60). Femoralis plaques (n = 12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm(2), P = 0.02; CD83: 19 versus 5 cells/0.1 mm(2), P = 0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus

Frequency of circulating mDCPs, pDCPs, and tDCPs in patients with PAD compared with healthy controls. Relative numbers of circulating mDCPs (a), pDCPs (b), and tDCPs (c) shown as percentage of white blood cell count (% of WBCs) and absolute numbers of circulating mDCPs (d), pDCPs (e), and tDCPs (f) (cells/μL) are shown. The box plots indicate the median (line inside the box), 25th and 75th percentile (lower and upper boundary of the box), and 10th and 90th percentile (whiskers outside box).
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fig2: Frequency of circulating mDCPs, pDCPs, and tDCPs in patients with PAD compared with healthy controls. Relative numbers of circulating mDCPs (a), pDCPs (b), and tDCPs (c) shown as percentage of white blood cell count (% of WBCs) and absolute numbers of circulating mDCPs (d), pDCPs (e), and tDCPs (f) (cells/μL) are shown. The box plots indicate the median (line inside the box), 25th and 75th percentile (lower and upper boundary of the box), and 10th and 90th percentile (whiskers outside box).

Mentions: In patients with PAD, a highly significant decrease in the relative numbers of circulating mDCPs (0.15 versus 0.2; P < 0.0001), pDCPs (0.09 versus 0.12; P = 0.01), and tDCPs (0.25 versus 0.36; P < 0.0001) was observed compared to the controls (Figure 2). Total numbers of mDCPs (10.38 versus 15.00 cells/μL; P < 0.0001), pDCPs (6.72 versus 8.40 cells/μL; P = 0.03), and tDCPs (17.84 versus 24.54 cells/μL; P < 0.0001) were also significantly decreased in patients with PAD.


Decrease in circulating dendritic cell precursors in patients with peripheral artery disease.

Kretzschmar D, Rohm I, Schäller S, Betge S, Pistulli R, Atiskova Y, Figulla HR, Yilmaz A - Mediators Inflamm. (2015)

Frequency of circulating mDCPs, pDCPs, and tDCPs in patients with PAD compared with healthy controls. Relative numbers of circulating mDCPs (a), pDCPs (b), and tDCPs (c) shown as percentage of white blood cell count (% of WBCs) and absolute numbers of circulating mDCPs (d), pDCPs (e), and tDCPs (f) (cells/μL) are shown. The box plots indicate the median (line inside the box), 25th and 75th percentile (lower and upper boundary of the box), and 10th and 90th percentile (whiskers outside box).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4413958&req=5

fig2: Frequency of circulating mDCPs, pDCPs, and tDCPs in patients with PAD compared with healthy controls. Relative numbers of circulating mDCPs (a), pDCPs (b), and tDCPs (c) shown as percentage of white blood cell count (% of WBCs) and absolute numbers of circulating mDCPs (d), pDCPs (e), and tDCPs (f) (cells/μL) are shown. The box plots indicate the median (line inside the box), 25th and 75th percentile (lower and upper boundary of the box), and 10th and 90th percentile (whiskers outside box).
Mentions: In patients with PAD, a highly significant decrease in the relative numbers of circulating mDCPs (0.15 versus 0.2; P < 0.0001), pDCPs (0.09 versus 0.12; P = 0.01), and tDCPs (0.25 versus 0.36; P < 0.0001) was observed compared to the controls (Figure 2). Total numbers of mDCPs (10.38 versus 15.00 cells/μL; P < 0.0001), pDCPs (6.72 versus 8.40 cells/μL; P = 0.03), and tDCPs (17.84 versus 24.54 cells/μL; P < 0.0001) were also significantly decreased in patients with PAD.

Bottom Line: In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed.We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD.Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany.

ABSTRACT
Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n = 52) compared to controls (n = 60). Femoralis plaques (n = 12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm(2), P = 0.02; CD83: 19 versus 5 cells/0.1 mm(2), P = 0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus