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Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development.

Li SS, Wang LS, Shu QY, Wu J, Chen LG, Shao S, Yin DD - BMC Genomics (2015)

Bottom Line: Therefore, transcriptome data is needed to better understand the underlying mechanisms.These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation.Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. shshli@ibcas.ac.cn.

ABSTRACT

Background: Tree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms.

Results: In this study, lipid accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.

Conclusions: This study provides the first comprehensive genomic resources characterizing tree peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.

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Venn diagram of transcripts (both identified and unknown) that were up- or down-regulated between seeds of 60 DAP vs 30 DAP (A) and 90 DAP vs 30 DAP (B). The “+” and “−” indicate up- and down-regulated transcripts, respectively. A total of 2133 transcripts were significantly (FDR < 0.05) expressed during developing seeds. 186: unique up-regulated transcripts in seeds of 60 DAP vs 30 DAP; 619: unique up-regulated transcripts in seeds of 90 DAP vs 30 DAP; 131: unique down-regulated transcripts in seeds of 60 DAP vs 30 DAP; 831: unique down-regulated transcripts in seeds of 90 DAP vs 30 DAP; 168: commonly up-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 197: commonly down-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 1: down-regulated in seeds of 60 DAP vs 30 DAP but up-regulated in seeds of 90 DAP vs 30 DAP.
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Fig5: Venn diagram of transcripts (both identified and unknown) that were up- or down-regulated between seeds of 60 DAP vs 30 DAP (A) and 90 DAP vs 30 DAP (B). The “+” and “−” indicate up- and down-regulated transcripts, respectively. A total of 2133 transcripts were significantly (FDR < 0.05) expressed during developing seeds. 186: unique up-regulated transcripts in seeds of 60 DAP vs 30 DAP; 619: unique up-regulated transcripts in seeds of 90 DAP vs 30 DAP; 131: unique down-regulated transcripts in seeds of 60 DAP vs 30 DAP; 831: unique down-regulated transcripts in seeds of 90 DAP vs 30 DAP; 168: commonly up-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 197: commonly down-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 1: down-regulated in seeds of 60 DAP vs 30 DAP but up-regulated in seeds of 90 DAP vs 30 DAP.

Mentions: Tissues at 30 days after pollination (DAP) were served as the control, and a total of 683 and 1816 differentially expressed unigenes were identified at 60 and 90 DAP, respectively. Among them, 366 unigenes were mutually present in both contrast groups, while 317 and 1450 were specifically expressed only in the 60 vs 30 DAP and 90 vs 30 DAP groups, respectively (Figure 5).Figure 5


Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development.

Li SS, Wang LS, Shu QY, Wu J, Chen LG, Shao S, Yin DD - BMC Genomics (2015)

Venn diagram of transcripts (both identified and unknown) that were up- or down-regulated between seeds of 60 DAP vs 30 DAP (A) and 90 DAP vs 30 DAP (B). The “+” and “−” indicate up- and down-regulated transcripts, respectively. A total of 2133 transcripts were significantly (FDR < 0.05) expressed during developing seeds. 186: unique up-regulated transcripts in seeds of 60 DAP vs 30 DAP; 619: unique up-regulated transcripts in seeds of 90 DAP vs 30 DAP; 131: unique down-regulated transcripts in seeds of 60 DAP vs 30 DAP; 831: unique down-regulated transcripts in seeds of 90 DAP vs 30 DAP; 168: commonly up-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 197: commonly down-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 1: down-regulated in seeds of 60 DAP vs 30 DAP but up-regulated in seeds of 90 DAP vs 30 DAP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4404109&req=5

Fig5: Venn diagram of transcripts (both identified and unknown) that were up- or down-regulated between seeds of 60 DAP vs 30 DAP (A) and 90 DAP vs 30 DAP (B). The “+” and “−” indicate up- and down-regulated transcripts, respectively. A total of 2133 transcripts were significantly (FDR < 0.05) expressed during developing seeds. 186: unique up-regulated transcripts in seeds of 60 DAP vs 30 DAP; 619: unique up-regulated transcripts in seeds of 90 DAP vs 30 DAP; 131: unique down-regulated transcripts in seeds of 60 DAP vs 30 DAP; 831: unique down-regulated transcripts in seeds of 90 DAP vs 30 DAP; 168: commonly up-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 197: commonly down-regulated transcripts between seeds of 60 DAP vs 30 DAP and 90 DAP vs 30 DAP; 1: down-regulated in seeds of 60 DAP vs 30 DAP but up-regulated in seeds of 90 DAP vs 30 DAP.
Mentions: Tissues at 30 days after pollination (DAP) were served as the control, and a total of 683 and 1816 differentially expressed unigenes were identified at 60 and 90 DAP, respectively. Among them, 366 unigenes were mutually present in both contrast groups, while 317 and 1450 were specifically expressed only in the 60 vs 30 DAP and 90 vs 30 DAP groups, respectively (Figure 5).Figure 5

Bottom Line: Therefore, transcriptome data is needed to better understand the underlying mechanisms.These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation.Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. shshli@ibcas.ac.cn.

ABSTRACT

Background: Tree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms.

Results: In this study, lipid accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.

Conclusions: This study provides the first comprehensive genomic resources characterizing tree peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.

Show MeSH
Related in: MedlinePlus