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Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

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Related in: MedlinePlus

Urease activity in the rumen after immunization (A) and ammonia concentration variation after urea was infused into the rumen (B). Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the time points.
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Fig4: Urease activity in the rumen after immunization (A) and ammonia concentration variation after urea was infused into the rumen (B). Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the time points.

Mentions: The effect of immunization against urease was assessed by analyzing rumen fermentation characteristic and ureolysis in the rumen of the vaccinated cows. No significant difference in rumen urease activity was seen between the control and the vaccinated groups from days 0 to 35 (before the 3rd booster) (Figure 4A). At day 49 (two weeks after the third booster), however, urease activity in the vaccinated group was 17% lower (P < 0.01) than that in the control group. Rumen pH and volatile fatty acid (VFA) concentration were not affected by the immunization (see Additional file 2). After direct infusion of urea into the rumen at day 56, ammonia concentration in the rumen ascended during the first hour and then descended to the pre-infusion level (Figure 4B). Compared to the control group, the vaccinated group had lower (P < 0.01) ammonia concentration at 1 and 2 h post infusion, but not thereafter.Figure 4


Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

Urease activity in the rumen after immunization (A) and ammonia concentration variation after urea was infused into the rumen (B). Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the time points.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4404106&req=5

Fig4: Urease activity in the rumen after immunization (A) and ammonia concentration variation after urea was infused into the rumen (B). Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the time points.
Mentions: The effect of immunization against urease was assessed by analyzing rumen fermentation characteristic and ureolysis in the rumen of the vaccinated cows. No significant difference in rumen urease activity was seen between the control and the vaccinated groups from days 0 to 35 (before the 3rd booster) (Figure 4A). At day 49 (two weeks after the third booster), however, urease activity in the vaccinated group was 17% lower (P < 0.01) than that in the control group. Rumen pH and volatile fatty acid (VFA) concentration were not affected by the immunization (see Additional file 2). After direct infusion of urea into the rumen at day 56, ammonia concentration in the rumen ascended during the first hour and then descended to the pre-infusion level (Figure 4B). Compared to the control group, the vaccinated group had lower (P < 0.01) ammonia concentration at 1 and 2 h post infusion, but not thereafter.Figure 4

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

Show MeSH
Related in: MedlinePlus