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Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

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Related in: MedlinePlus

Titers of IgG (A and C) and IgA (B and D) in the serum (A and B) and the saliva (C and D) of cows. Arrow indicates days of vaccinations. Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the same days.
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Fig3: Titers of IgG (A and C) and IgA (B and D) in the serum (A and B) and the saliva (C and D) of cows. Arrow indicates days of vaccinations. Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the same days.

Mentions: After the immunization with H. pylori UreC, no apparent adverse effect was seen on health, milk production, or digestion of dry matter and crude protein (data not shown). Low titers of anti-urease antibody were detected in the serum and the saliva samples from the control group from day 0 (prior to mock immunization) to day 49 (Figure 3). Compared to the control group, the vaccinated group had higher (P < 0.01) serum titers of both IgG and IgA from day 7 onward, while higher (P < 0.01) saliva titers of IgG and IgA were noted from days 21 and 7 onward, respectively. The IgA titer peaked at day 35 in both the serum and the saliva, but the IgG titers peaked later at day 49. The variation of both IgA and IgG titers had similar trends in the serum and the saliva. The highest titers of both IgG and IgA in the serum were 13- and 20-fold greater, respectively, than those noted for the saliva.Figure 3


Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

Titers of IgG (A and C) and IgA (B and D) in the serum (A and B) and the saliva (C and D) of cows. Arrow indicates days of vaccinations. Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the same days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4404106&req=5

Fig3: Titers of IgG (A and C) and IgA (B and D) in the serum (A and B) and the saliva (C and D) of cows. Arrow indicates days of vaccinations. Values are means (n = 4), with error bars representing standard deviation. The asterisks (*) indicate significant (P < 0.05) difference between the control group and the vaccinated group at the same days.
Mentions: After the immunization with H. pylori UreC, no apparent adverse effect was seen on health, milk production, or digestion of dry matter and crude protein (data not shown). Low titers of anti-urease antibody were detected in the serum and the saliva samples from the control group from day 0 (prior to mock immunization) to day 49 (Figure 3). Compared to the control group, the vaccinated group had higher (P < 0.01) serum titers of both IgG and IgA from day 7 onward, while higher (P < 0.01) saliva titers of IgG and IgA were noted from days 21 and 7 onward, respectively. The IgA titer peaked at day 35 in both the serum and the saliva, but the IgG titers peaked later at day 49. The variation of both IgA and IgG titers had similar trends in the serum and the saliva. The highest titers of both IgG and IgA in the serum were 13- and 20-fold greater, respectively, than those noted for the saliva.Figure 3

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

Show MeSH
Related in: MedlinePlus