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Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

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Related in: MedlinePlus

A neighbor-joining tree of the UreC sequences recovered from rumen digesta. The consensus tree was constructed from amino acid sequences inferred from the ureC sequences recovered from rumen and known bacterial species. Bootstrap values were calculated from 1,000 trees. Only bootstrapping values greater than 50% are shown.
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Fig1: A neighbor-joining tree of the UreC sequences recovered from rumen digesta. The consensus tree was constructed from amino acid sequences inferred from the ureC sequences recovered from rumen and known bacterial species. Bootstrap values were calculated from 1,000 trees. Only bootstrapping values greater than 50% are shown.

Mentions: The ureC diversity in the rumen was examined by cloning and sequencing of ureC genes using degenerate primers. In total, 317 ureC sequences were obtained from the microbial DNA of rumen digesta of Chinese Holstein cows. Phylogenetic analysis revealed five ureC clusters (FigureĀ 1). Cluster I contained 203 (64% of total sequences) of the ureC sequences, and it was about 84% identical (based on amino acid sequence) to the ureC gene of Helicobacter pylori (H. pylori). Clusters IIa and IIb represented 29 (9%) and 42 (13%) of the ureC sequences, respectively, and both were closely related (98-100% aa sequence identity) to the ureC of H. pylori. Clusters III and IV, each of which contained a small number of ureC sequences, and cluster V, represent the rest of the ureC sequences, had no match with any known ureC sequences.Figure 1


Reducing microbial ureolytic activity in the rumen by immunization against urease therein.

Zhao S, Wang J, Zheng N, Bu D, Sun P, Yu Z - BMC Vet. Res. (2015)

A neighbor-joining tree of the UreC sequences recovered from rumen digesta. The consensus tree was constructed from amino acid sequences inferred from the ureC sequences recovered from rumen and known bacterial species. Bootstrap values were calculated from 1,000 trees. Only bootstrapping values greater than 50% are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4404106&req=5

Fig1: A neighbor-joining tree of the UreC sequences recovered from rumen digesta. The consensus tree was constructed from amino acid sequences inferred from the ureC sequences recovered from rumen and known bacterial species. Bootstrap values were calculated from 1,000 trees. Only bootstrapping values greater than 50% are shown.
Mentions: The ureC diversity in the rumen was examined by cloning and sequencing of ureC genes using degenerate primers. In total, 317 ureC sequences were obtained from the microbial DNA of rumen digesta of Chinese Holstein cows. Phylogenetic analysis revealed five ureC clusters (FigureĀ 1). Cluster I contained 203 (64% of total sequences) of the ureC sequences, and it was about 84% identical (based on amino acid sequence) to the ureC gene of Helicobacter pylori (H. pylori). Clusters IIa and IIb represented 29 (9%) and 42 (13%) of the ureC sequences, respectively, and both were closely related (98-100% aa sequence identity) to the ureC of H. pylori. Clusters III and IV, each of which contained a small number of ureC sequences, and cluster V, represent the rest of the ureC sequences, had no match with any known ureC sequences.Figure 1

Bottom Line: The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro.Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanyingyuan West Road, Beijing, 100193, PR China. 13811307631@163.com.

ABSTRACT

Background: Ureolytic activity of rumen bacteria leads to rapid urea conversion to ammonia in the rumen of dairy cows, resulting possible toxicity, excessive ammonia excretion to the environment, and poor nitrogen utilization. The present study investigated immunization of dairy cows against urease in the rumen as an approach to mitigate bacterial ureolytic activity therein.

Results: Most alpha subunit of rumen urease (UreC) proteins shared very similar amino acid sequences, which were also highly similar to that of H. pylori. Anti-urease titers in the serum and the saliva of the immunized cows were evaluated following repeated immunization with the UreC of H. pylori as the vaccine. After the fourth booster, the vaccinated cows had a significantly reduced urease activity (by 17%) in the rumen than the control cows that were mock immunized cows. The anti-urease antibody significantly reduced ureolysis and corresponding ammonia formation in rumen fluid in vitro. Western blotting revealed that the H. pylori UreC had high immunological homology with the UreC from rumen bacteria.

Conclusions: Vaccine developed based on UreC of H. pylori can be a useful approach to decrease bacterial ureolysis in the rumen.

Show MeSH
Related in: MedlinePlus