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Catalases Induction in High Virulence Pinewood Nematode Bursaphelenchus xylophilus under Hydrogen Peroxide-Induced Stress.

Vicente CS, Ikuyo Y, Shinya R, Mota M, Hasegawa K - PLoS ONE (2015)

Bottom Line: Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree.Both catalases are expressed throughout the nematode intestine.Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, Kasugai, Aichi, Japan; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Évora, Portugal.

ABSTRACT
Considered an EPPO A2 quarantine pest, Bursaphelenchus xylophilus is the causal agent of the pine wilt disease and the most devastating plant parasitic nematode attacking coniferous trees in the world. In the early stages of invasion, this nematode has to manage host defence mechanisms, such as strong oxidative stress. Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree. In this work, our main objective was to understand to what extent B. xylophilus catalases are involved in their tolerance to oxidative stress and virulence, using as oxidant agent the reactive oxygen species hydrogen peroxide (H2O2). After 24 hours of exposure, high virulence isolates of B. xylophilus could withstand higher H2O2 concentrations in comparison with low virulence B. xylophilus and B. mucronatus, corroborating our observation of Bxy-ctl-1 and Bxy-ctl-2 catalase up-regulation under the same experimental conditions. Both catalases are expressed throughout the nematode intestine. In addition, transgenic strains of Caenorhabditis elegans overexpressing B. xylophilus catalases were constructed and evaluated for survival under similar conditions as previously. Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

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Related in: MedlinePlus

Expression patterns of Bxy-ctl-1::gfp (A) and Bxy-ctl-2::gfp (B) in, respectively, transgenic C. elegans KHA149 and KHA151.Differential interference contrast (DIC) microscope images and, DIC and fluorescence-merged images (DIC+FL) of C. elegans head, vulva and tail region. Scale bars, 100 μm.
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pone.0123839.g005: Expression patterns of Bxy-ctl-1::gfp (A) and Bxy-ctl-2::gfp (B) in, respectively, transgenic C. elegans KHA149 and KHA151.Differential interference contrast (DIC) microscope images and, DIC and fluorescence-merged images (DIC+FL) of C. elegans head, vulva and tail region. Scale bars, 100 μm.

Mentions: Under the control of C. elegans promoters, B. xylophilus Bxy-ctl-1 and Bxt-ctl-2 showed different spatial expression patterns; ctl-1 was mainly detected in the cytosol of the intestine, and ctl-2 was detected as high levels along the nervous system and pharynx (Fig 5A and 5B). These expression patterns were the same with those previously reported [31]. Although these transgenic C. elegans were extrachromosomal lines with unstable transgene transmission, the expression patterns were similar between the transgenic lines KHA149 and 150, and between KHA151 and 152 (data not shown). We used KHA149 and 151 for further experiments.


Catalases Induction in High Virulence Pinewood Nematode Bursaphelenchus xylophilus under Hydrogen Peroxide-Induced Stress.

Vicente CS, Ikuyo Y, Shinya R, Mota M, Hasegawa K - PLoS ONE (2015)

Expression patterns of Bxy-ctl-1::gfp (A) and Bxy-ctl-2::gfp (B) in, respectively, transgenic C. elegans KHA149 and KHA151.Differential interference contrast (DIC) microscope images and, DIC and fluorescence-merged images (DIC+FL) of C. elegans head, vulva and tail region. Scale bars, 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4404050&req=5

pone.0123839.g005: Expression patterns of Bxy-ctl-1::gfp (A) and Bxy-ctl-2::gfp (B) in, respectively, transgenic C. elegans KHA149 and KHA151.Differential interference contrast (DIC) microscope images and, DIC and fluorescence-merged images (DIC+FL) of C. elegans head, vulva and tail region. Scale bars, 100 μm.
Mentions: Under the control of C. elegans promoters, B. xylophilus Bxy-ctl-1 and Bxt-ctl-2 showed different spatial expression patterns; ctl-1 was mainly detected in the cytosol of the intestine, and ctl-2 was detected as high levels along the nervous system and pharynx (Fig 5A and 5B). These expression patterns were the same with those previously reported [31]. Although these transgenic C. elegans were extrachromosomal lines with unstable transgene transmission, the expression patterns were similar between the transgenic lines KHA149 and 150, and between KHA151 and 152 (data not shown). We used KHA149 and 151 for further experiments.

Bottom Line: Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree.Both catalases are expressed throughout the nematode intestine.Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, Kasugai, Aichi, Japan; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Évora, Portugal.

ABSTRACT
Considered an EPPO A2 quarantine pest, Bursaphelenchus xylophilus is the causal agent of the pine wilt disease and the most devastating plant parasitic nematode attacking coniferous trees in the world. In the early stages of invasion, this nematode has to manage host defence mechanisms, such as strong oxidative stress. Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree. In this work, our main objective was to understand to what extent B. xylophilus catalases are involved in their tolerance to oxidative stress and virulence, using as oxidant agent the reactive oxygen species hydrogen peroxide (H2O2). After 24 hours of exposure, high virulence isolates of B. xylophilus could withstand higher H2O2 concentrations in comparison with low virulence B. xylophilus and B. mucronatus, corroborating our observation of Bxy-ctl-1 and Bxy-ctl-2 catalase up-regulation under the same experimental conditions. Both catalases are expressed throughout the nematode intestine. In addition, transgenic strains of Caenorhabditis elegans overexpressing B. xylophilus catalases were constructed and evaluated for survival under similar conditions as previously. Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

Show MeSH
Related in: MedlinePlus