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Catalases Induction in High Virulence Pinewood Nematode Bursaphelenchus xylophilus under Hydrogen Peroxide-Induced Stress.

Vicente CS, Ikuyo Y, Shinya R, Mota M, Hasegawa K - PLoS ONE (2015)

Bottom Line: Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree.Both catalases are expressed throughout the nematode intestine.Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, Kasugai, Aichi, Japan; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Évora, Portugal.

ABSTRACT
Considered an EPPO A2 quarantine pest, Bursaphelenchus xylophilus is the causal agent of the pine wilt disease and the most devastating plant parasitic nematode attacking coniferous trees in the world. In the early stages of invasion, this nematode has to manage host defence mechanisms, such as strong oxidative stress. Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree. In this work, our main objective was to understand to what extent B. xylophilus catalases are involved in their tolerance to oxidative stress and virulence, using as oxidant agent the reactive oxygen species hydrogen peroxide (H2O2). After 24 hours of exposure, high virulence isolates of B. xylophilus could withstand higher H2O2 concentrations in comparison with low virulence B. xylophilus and B. mucronatus, corroborating our observation of Bxy-ctl-1 and Bxy-ctl-2 catalase up-regulation under the same experimental conditions. Both catalases are expressed throughout the nematode intestine. In addition, transgenic strains of Caenorhabditis elegans overexpressing B. xylophilus catalases were constructed and evaluated for survival under similar conditions as previously. Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

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mRNA expression patterns of Bxy-ctl-1 (A), Bxy-ctl-2 (B), and Bxy-eng-1 (D) in B. xylophilus Ka4.No expression signal was observed with Bxy-ctl-1 sense probe (C). Light microscope images of B. xylophilus head region. Scale bars, 100 μm.
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pone.0123839.g004: mRNA expression patterns of Bxy-ctl-1 (A), Bxy-ctl-2 (B), and Bxy-eng-1 (D) in B. xylophilus Ka4.No expression signal was observed with Bxy-ctl-1 sense probe (C). Light microscope images of B. xylophilus head region. Scale bars, 100 μm.

Mentions: Bxy-ctl-1 and Bxy-ctl-2 messenger RNAs presented the same expression pattern in the nematode’s intestine (Fig 4A and 4B). Bxy-eng-1, profusely expressed in B. xylophilus oesophageal glands, was used as a positive control (Fig 4D) [29]. No hybridization was observed for the control sense probe of Bxy-ctl-1 (Fig 4C), Bxy-ctl-2 or Bxy-egn-1 (data not shown).


Catalases Induction in High Virulence Pinewood Nematode Bursaphelenchus xylophilus under Hydrogen Peroxide-Induced Stress.

Vicente CS, Ikuyo Y, Shinya R, Mota M, Hasegawa K - PLoS ONE (2015)

mRNA expression patterns of Bxy-ctl-1 (A), Bxy-ctl-2 (B), and Bxy-eng-1 (D) in B. xylophilus Ka4.No expression signal was observed with Bxy-ctl-1 sense probe (C). Light microscope images of B. xylophilus head region. Scale bars, 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4404050&req=5

pone.0123839.g004: mRNA expression patterns of Bxy-ctl-1 (A), Bxy-ctl-2 (B), and Bxy-eng-1 (D) in B. xylophilus Ka4.No expression signal was observed with Bxy-ctl-1 sense probe (C). Light microscope images of B. xylophilus head region. Scale bars, 100 μm.
Mentions: Bxy-ctl-1 and Bxy-ctl-2 messenger RNAs presented the same expression pattern in the nematode’s intestine (Fig 4A and 4B). Bxy-eng-1, profusely expressed in B. xylophilus oesophageal glands, was used as a positive control (Fig 4D) [29]. No hybridization was observed for the control sense probe of Bxy-ctl-1 (Fig 4C), Bxy-ctl-2 or Bxy-egn-1 (data not shown).

Bottom Line: Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree.Both catalases are expressed throughout the nematode intestine.Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, Kasugai, Aichi, Japan; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Évora, Portugal.

ABSTRACT
Considered an EPPO A2 quarantine pest, Bursaphelenchus xylophilus is the causal agent of the pine wilt disease and the most devastating plant parasitic nematode attacking coniferous trees in the world. In the early stages of invasion, this nematode has to manage host defence mechanisms, such as strong oxidative stress. Only successful, virulent nematodes are able to tolerate the basal plant defences, and furthermore migrate and proliferate inside of the host tree. In this work, our main objective was to understand to what extent B. xylophilus catalases are involved in their tolerance to oxidative stress and virulence, using as oxidant agent the reactive oxygen species hydrogen peroxide (H2O2). After 24 hours of exposure, high virulence isolates of B. xylophilus could withstand higher H2O2 concentrations in comparison with low virulence B. xylophilus and B. mucronatus, corroborating our observation of Bxy-ctl-1 and Bxy-ctl-2 catalase up-regulation under the same experimental conditions. Both catalases are expressed throughout the nematode intestine. In addition, transgenic strains of Caenorhabditis elegans overexpressing B. xylophilus catalases were constructed and evaluated for survival under similar conditions as previously. Our results suggest that catalases of high virulence B. xylophilus were crucial for nematode survival under prolonged exposure to in vitro oxidative stress, highlighting their adaptive response, which could contribute to their success in host conditions.

Show MeSH
Related in: MedlinePlus