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Mongolian Almond (Prunus mongolica Maxim): The Morpho-Physiological, Biochemical and Transcriptomic Response to Drought Stress.

Wang J, Zheng R, Bai S, Gao X, Liu M, Yan W - PLoS ONE (2015)

Bottom Line: The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert.We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings.Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.

ABSTRACT
Prunus mongolica Maxim, which is widely established in the Gobi Desert, shows extreme tolerance to drought. However, there is a lack of available transcriptomic resources for this species related to its response to water deficiency. To investigate the mechanisms that allow P. mongolica to maintain growth in extremely arid environments, the response of P. mongolica seedlings to drought stress was analyzed using morphological, physiological, biochemical and high-throughput sequencing approaches. We generated 28,713,735 and 26,650,133 raw reads from no-stress control and drought-stressed P. mongolica seedlings, respectively. In total, we obtained 67,352 transcripts with an average length of 874.44 bp. Compared with the no-stress control, 3,365 transcripts were differentially expressed in the drought-stressed seedlings, including 55.75% (1,876 transcripts) up-regulated and 44.25% (1,489 transcripts) down-regulated transcripts. The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert. Next, we analyzed the aquaporin and superoxide dismutase gene families due to their importance in plant resistance to drought stress. We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings. In addition, activation of iron superoxide dismutase transcription and enhanced transcription of manganese superoxide dismutase were observed in P. mongolica to promote tolerance of drought stress. This study identified drought response genes in P. mongolica seedlings. Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants.

No MeSH data available.


Related in: MedlinePlus

Size distribution of the total P. mongolica transcripts.
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pone.0124442.g002: Size distribution of the total P. mongolica transcripts.

Mentions: A total of 28,713,735 and 26,650,133 raw reads were generated from the WW and DS P. mongolica seedlings, respectively. After removing low-quality reads, a total of 26,851,249 clean reads were obtained for the WW treatment and 24,826,653 clean reads were obtained for the DS treatment. To generate more complete and representative information about the P. mongolica transcriptome, all of the clean reads from both libraries were mapped to transcripts. A total of 67,352 transcripts were obtained, ranging from 0.2 to 2.5 kb, with an average length 874.44 bp (Fig 2). Compared with previous studies [11,16,17] conducted using the 454 platform, both our results and the results of other studies [39,40] employing the same Solexa platforms show that Solexa sequencing technology can generate more clean reads mapped to transcripts and can be widely applied in non-model plant transcriptome sequencing. In addition, 63,613 transcripts were assembled in the DS library, which was a greater number than in the WW library, with 62,107 transcripts. Our results were consistent with previous conclusions indicating an activated transcriptome in plants in response to drought stress [17,41].


Mongolian Almond (Prunus mongolica Maxim): The Morpho-Physiological, Biochemical and Transcriptomic Response to Drought Stress.

Wang J, Zheng R, Bai S, Gao X, Liu M, Yan W - PLoS ONE (2015)

Size distribution of the total P. mongolica transcripts.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4404049&req=5

pone.0124442.g002: Size distribution of the total P. mongolica transcripts.
Mentions: A total of 28,713,735 and 26,650,133 raw reads were generated from the WW and DS P. mongolica seedlings, respectively. After removing low-quality reads, a total of 26,851,249 clean reads were obtained for the WW treatment and 24,826,653 clean reads were obtained for the DS treatment. To generate more complete and representative information about the P. mongolica transcriptome, all of the clean reads from both libraries were mapped to transcripts. A total of 67,352 transcripts were obtained, ranging from 0.2 to 2.5 kb, with an average length 874.44 bp (Fig 2). Compared with previous studies [11,16,17] conducted using the 454 platform, both our results and the results of other studies [39,40] employing the same Solexa platforms show that Solexa sequencing technology can generate more clean reads mapped to transcripts and can be widely applied in non-model plant transcriptome sequencing. In addition, 63,613 transcripts were assembled in the DS library, which was a greater number than in the WW library, with 62,107 transcripts. Our results were consistent with previous conclusions indicating an activated transcriptome in plants in response to drought stress [17,41].

Bottom Line: The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert.We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings.Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants.

View Article: PubMed Central - PubMed

Affiliation: College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.

ABSTRACT
Prunus mongolica Maxim, which is widely established in the Gobi Desert, shows extreme tolerance to drought. However, there is a lack of available transcriptomic resources for this species related to its response to water deficiency. To investigate the mechanisms that allow P. mongolica to maintain growth in extremely arid environments, the response of P. mongolica seedlings to drought stress was analyzed using morphological, physiological, biochemical and high-throughput sequencing approaches. We generated 28,713,735 and 26,650,133 raw reads from no-stress control and drought-stressed P. mongolica seedlings, respectively. In total, we obtained 67,352 transcripts with an average length of 874.44 bp. Compared with the no-stress control, 3,365 transcripts were differentially expressed in the drought-stressed seedlings, including 55.75% (1,876 transcripts) up-regulated and 44.25% (1,489 transcripts) down-regulated transcripts. The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert. Next, we analyzed the aquaporin and superoxide dismutase gene families due to their importance in plant resistance to drought stress. We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings. In addition, activation of iron superoxide dismutase transcription and enhanced transcription of manganese superoxide dismutase were observed in P. mongolica to promote tolerance of drought stress. This study identified drought response genes in P. mongolica seedlings. Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants.

No MeSH data available.


Related in: MedlinePlus