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Naturally-acquired cellular immune response against Plasmodium vivax merozoite surface protein-1 paralog antigen.

Changrob S, Leepiyasakulchai C, Tsuboi T, Cheng Y, Lim CS, Chootong P, Han ET - Malar. J. (2015)

Bottom Line: Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice.PvMSP1P-19 strongly induces a specific cellular immune response for protection against P. vivax compared with PvDBPII as the antigen induces activation of IFN-γ-producing effector cells following natural P. vivax exposure.Upon stimulation, PvMSP1P-19 has the potential to activate the recall response of Th1 effector memory cells that play a role in killing the parasite.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. siriruk.ch@gmail.com.

ABSTRACT

Background: Plasmodium vivax merozoite surface protein-1 paralog (PvMSP1P) is a glycosylphosphatidylinositol-anchored protein expressed on the merozoite surface. This molecule is a target of natural immunity, as high anti-MSP1P-19 antibody levels were detected during P. vivax infection and the antibody inhibited PvMSP1P-erythrocyte binding. Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice. The present study was performed to characterize natural cellular immunity against PvMSP1P-19 and PvDBP region II in acute and recovery P. vivax infection.

Methods: Peripheral blood mononuclear cells (PBMCs) from acute and recovery P. vivax infection were obtained for lymphocyte proliferation assay upon PvMSP1P-19 and PvDBP region II antigen stimulation. The culture supernatant was examined for the presence of the cytokines IL-2, TNF, IFN-γ and IL-10 by enzyme-linked immunosorbent assay (ELISA). To determine whether Th1 or Th2 have a memory response against PvMSP1P-19 and PvDBPII protein antigen, PBMCs from subjects who had recovered from P. vivax infection 8-10 weeks prior to the study were obtained for lymphocyte proliferation assay. Cytokine-producing cells were analysed by flow cytometry.

Results: IL-2 was detected at high levels in lymphocyte cultures from acutely infected P. vivax patients upon PvMSP1P-19 stimulation. Analysis of the Th1 or Th2 memory response in PBMC cultures from subjects who had recovered from P. vivax infection showed significantly elevated levels of PvMSP1P-19 and PvDBPII-specific IFN-γ-producing cells (P  <  0.05). Interestingly, the response of IFN-γ-producing cells in PvMSP1P stimulation was fourfold greater in recovered subjects than that in acute-infection patients. CD4(+) T cells were the major cell phenotype involved in the response to PvMSP1P-19 and PvDBPII antigen.

Conclusions: PvMSP1P-19 strongly induces a specific cellular immune response for protection against P. vivax compared with PvDBPII as the antigen induces activation of IFN-γ-producing effector cells following natural P. vivax exposure. Upon stimulation, PvMSP1P-19 has the potential to activate the recall response of Th1 effector memory cells that play a role in killing the parasite.

No MeSH data available.


Related in: MedlinePlus

Cytokine production of PvMSP1P-19-stimulated lymphocyte cultures obtained from individuals with acute P. vivax infection (n  =  15). PBMCs were re-stimulated with PvMSP1P-19 antigen for 48 h and culture supernatant was removed for cytokine detection. (a) IL-2 detection after 48 h of in vitro stimulation. (b) TNF, IFN-γ and IL-10 after 96 h of in vitro stimulation.
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Fig1: Cytokine production of PvMSP1P-19-stimulated lymphocyte cultures obtained from individuals with acute P. vivax infection (n  =  15). PBMCs were re-stimulated with PvMSP1P-19 antigen for 48 h and culture supernatant was removed for cytokine detection. (a) IL-2 detection after 48 h of in vitro stimulation. (b) TNF, IFN-γ and IL-10 after 96 h of in vitro stimulation.

Mentions: To evaluate the immunogenicity of PvMSP1P-19 in induction of PvMSP1P-19-specific T cell function during P. vivax infection, PBMCs from acutely infected P. vivax patients were subjected to lymphocyte proliferation assay. To detect lymphocyte proliferation upon PvMSP1P-19 stimulation, IL-2 levels in the culture supernatant were determined by ELISA. The results showed that PvMSP1P-19 significantly stimulated IL-2 production, indicating that PvMSP1P-19 induces lymphocyte proliferation (PvMSP1P-19  =  151.50  ±  132.96 pg/mL, unstimulated  =  64.88  ±  44.26 pg/mL, P  <  0.05, Figure 1a). However, the proinflammatory cytokine, TNF, was detected at low levels in cultures of lymphocytes from acute P. vivax patients (PvMSP1P-19  =  172.32  ±  175.86 pg/mL, unstimulated  =  126.64  ±  163.15, P  >  0.05).Figure 1


Naturally-acquired cellular immune response against Plasmodium vivax merozoite surface protein-1 paralog antigen.

Changrob S, Leepiyasakulchai C, Tsuboi T, Cheng Y, Lim CS, Chootong P, Han ET - Malar. J. (2015)

Cytokine production of PvMSP1P-19-stimulated lymphocyte cultures obtained from individuals with acute P. vivax infection (n  =  15). PBMCs were re-stimulated with PvMSP1P-19 antigen for 48 h and culture supernatant was removed for cytokine detection. (a) IL-2 detection after 48 h of in vitro stimulation. (b) TNF, IFN-γ and IL-10 after 96 h of in vitro stimulation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403936&req=5

Fig1: Cytokine production of PvMSP1P-19-stimulated lymphocyte cultures obtained from individuals with acute P. vivax infection (n  =  15). PBMCs were re-stimulated with PvMSP1P-19 antigen for 48 h and culture supernatant was removed for cytokine detection. (a) IL-2 detection after 48 h of in vitro stimulation. (b) TNF, IFN-γ and IL-10 after 96 h of in vitro stimulation.
Mentions: To evaluate the immunogenicity of PvMSP1P-19 in induction of PvMSP1P-19-specific T cell function during P. vivax infection, PBMCs from acutely infected P. vivax patients were subjected to lymphocyte proliferation assay. To detect lymphocyte proliferation upon PvMSP1P-19 stimulation, IL-2 levels in the culture supernatant were determined by ELISA. The results showed that PvMSP1P-19 significantly stimulated IL-2 production, indicating that PvMSP1P-19 induces lymphocyte proliferation (PvMSP1P-19  =  151.50  ±  132.96 pg/mL, unstimulated  =  64.88  ±  44.26 pg/mL, P  <  0.05, Figure 1a). However, the proinflammatory cytokine, TNF, was detected at low levels in cultures of lymphocytes from acute P. vivax patients (PvMSP1P-19  =  172.32  ±  175.86 pg/mL, unstimulated  =  126.64  ±  163.15, P  >  0.05).Figure 1

Bottom Line: Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice.PvMSP1P-19 strongly induces a specific cellular immune response for protection against P. vivax compared with PvDBPII as the antigen induces activation of IFN-γ-producing effector cells following natural P. vivax exposure.Upon stimulation, PvMSP1P-19 has the potential to activate the recall response of Th1 effector memory cells that play a role in killing the parasite.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. siriruk.ch@gmail.com.

ABSTRACT

Background: Plasmodium vivax merozoite surface protein-1 paralog (PvMSP1P) is a glycosylphosphatidylinositol-anchored protein expressed on the merozoite surface. This molecule is a target of natural immunity, as high anti-MSP1P-19 antibody levels were detected during P. vivax infection and the antibody inhibited PvMSP1P-erythrocyte binding. Recombinant PvMSP1P antigen results in production of a significant Th1 cytokine response in immunized mice. The present study was performed to characterize natural cellular immunity against PvMSP1P-19 and PvDBP region II in acute and recovery P. vivax infection.

Methods: Peripheral blood mononuclear cells (PBMCs) from acute and recovery P. vivax infection were obtained for lymphocyte proliferation assay upon PvMSP1P-19 and PvDBP region II antigen stimulation. The culture supernatant was examined for the presence of the cytokines IL-2, TNF, IFN-γ and IL-10 by enzyme-linked immunosorbent assay (ELISA). To determine whether Th1 or Th2 have a memory response against PvMSP1P-19 and PvDBPII protein antigen, PBMCs from subjects who had recovered from P. vivax infection 8-10 weeks prior to the study were obtained for lymphocyte proliferation assay. Cytokine-producing cells were analysed by flow cytometry.

Results: IL-2 was detected at high levels in lymphocyte cultures from acutely infected P. vivax patients upon PvMSP1P-19 stimulation. Analysis of the Th1 or Th2 memory response in PBMC cultures from subjects who had recovered from P. vivax infection showed significantly elevated levels of PvMSP1P-19 and PvDBPII-specific IFN-γ-producing cells (P  <  0.05). Interestingly, the response of IFN-γ-producing cells in PvMSP1P stimulation was fourfold greater in recovered subjects than that in acute-infection patients. CD4(+) T cells were the major cell phenotype involved in the response to PvMSP1P-19 and PvDBPII antigen.

Conclusions: PvMSP1P-19 strongly induces a specific cellular immune response for protection against P. vivax compared with PvDBPII as the antigen induces activation of IFN-γ-producing effector cells following natural P. vivax exposure. Upon stimulation, PvMSP1P-19 has the potential to activate the recall response of Th1 effector memory cells that play a role in killing the parasite.

No MeSH data available.


Related in: MedlinePlus