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Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi.

Valenzuela-Muñoz V, Sturm A, Gallardo-Escárate C - Parasit Vectors (2015)

Bottom Line: High transcript levels of the ABCB and ABCC subfamilies were evidenced.Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO. Box 160-C, Concepción, Chile. valevalenzuela@gmail.com.

ABSTRACT

Background: ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.

Methods: llumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.

Results: From the transcriptome database of C. rogercresseyi, 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. Moreover, RNA-Seq analysis was performed for adult male and female salmon lice exposed to the delousing drugs azamethiphos and deltamethrin. High transcript levels of the ABCB and ABCC subfamilies were evidenced. Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.

Conclusions: The present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

No MeSH data available.


Related in: MedlinePlus

Cluster of gene expression levels between adult controls, adults exposed to 3 ppb of azamethiphos, and adults exposed to 2 ppb of deltamethrin.
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Fig3: Cluster of gene expression levels between adult controls, adults exposed to 3 ppb of azamethiphos, and adults exposed to 2 ppb of deltamethrin.

Mentions: Comparing the transcriptomic response of C. rogercresseyi adults exposed to azamethiphos, deltamethrin and the control group, the adults exposed to deltamethrin and the control group presented similar expression profiles of ABC transporters (Figure 3). The ABCB and ABCC subfamilies were upregulated in adult sea lice exposed to azamethiphos (Table 3). Also, increased expression was observed for both females and males in contigs annotated as members of the ABCA/D subfamilies in response to azamethiphos (Figure 4). An opposite effect was observed in contig 1285 annotated as an ABCF transporter, which was downregulated in sea lice exposed to azamethiphos. Furthermore, different transcriptomic responses were observed between sexes in response to azamethiphos, where the ABCB and ABCC subfamilies were only up-regulated in males. In response to deltamethrin, the ABCD/E/F subfamilies were highly regulated in adult sea lice (Table 3). Expression profiles of ABC transporters between females and males were similar (Figure 5). However, the contig 4421 annotated for ABCB transporter was upregulated in males exposed to deltamethrin, while females presented a high expression of contig 3679 annotated as a member of the ABCF subfamily.Additionally, in order to validate the transcription profiles obtained from sequencing data analysis, RT-qPCR was conducted on one represent of ABC transporters subfamily. The Pearson correlation evidenced a high linear dependence of fold change values obtained from both RNA-Seq and RT-qPCR for each ABC transporters in sea louse exposed to azamethiphos and deltamethrin (Figure 6).Figure 3


Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi.

Valenzuela-Muñoz V, Sturm A, Gallardo-Escárate C - Parasit Vectors (2015)

Cluster of gene expression levels between adult controls, adults exposed to 3 ppb of azamethiphos, and adults exposed to 2 ppb of deltamethrin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403882&req=5

Fig3: Cluster of gene expression levels between adult controls, adults exposed to 3 ppb of azamethiphos, and adults exposed to 2 ppb of deltamethrin.
Mentions: Comparing the transcriptomic response of C. rogercresseyi adults exposed to azamethiphos, deltamethrin and the control group, the adults exposed to deltamethrin and the control group presented similar expression profiles of ABC transporters (Figure 3). The ABCB and ABCC subfamilies were upregulated in adult sea lice exposed to azamethiphos (Table 3). Also, increased expression was observed for both females and males in contigs annotated as members of the ABCA/D subfamilies in response to azamethiphos (Figure 4). An opposite effect was observed in contig 1285 annotated as an ABCF transporter, which was downregulated in sea lice exposed to azamethiphos. Furthermore, different transcriptomic responses were observed between sexes in response to azamethiphos, where the ABCB and ABCC subfamilies were only up-regulated in males. In response to deltamethrin, the ABCD/E/F subfamilies were highly regulated in adult sea lice (Table 3). Expression profiles of ABC transporters between females and males were similar (Figure 5). However, the contig 4421 annotated for ABCB transporter was upregulated in males exposed to deltamethrin, while females presented a high expression of contig 3679 annotated as a member of the ABCF subfamily.Additionally, in order to validate the transcription profiles obtained from sequencing data analysis, RT-qPCR was conducted on one represent of ABC transporters subfamily. The Pearson correlation evidenced a high linear dependence of fold change values obtained from both RNA-Seq and RT-qPCR for each ABC transporters in sea louse exposed to azamethiphos and deltamethrin (Figure 6).Figure 3

Bottom Line: High transcript levels of the ABCB and ABCC subfamilies were evidenced.Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO. Box 160-C, Concepción, Chile. valevalenzuela@gmail.com.

ABSTRACT

Background: ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.

Methods: llumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.

Results: From the transcriptome database of C. rogercresseyi, 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. Moreover, RNA-Seq analysis was performed for adult male and female salmon lice exposed to the delousing drugs azamethiphos and deltamethrin. High transcript levels of the ABCB and ABCC subfamilies were evidenced. Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.

Conclusions: The present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

No MeSH data available.


Related in: MedlinePlus