Limits...
Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi.

Valenzuela-Muñoz V, Sturm A, Gallardo-Escárate C - Parasit Vectors (2015)

Bottom Line: High transcript levels of the ABCB and ABCC subfamilies were evidenced.Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO. Box 160-C, Concepción, Chile. valevalenzuela@gmail.com.

ABSTRACT

Background: ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.

Methods: llumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.

Results: From the transcriptome database of C. rogercresseyi, 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. Moreover, RNA-Seq analysis was performed for adult male and female salmon lice exposed to the delousing drugs azamethiphos and deltamethrin. High transcript levels of the ABCB and ABCC subfamilies were evidenced. Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.

Conclusions: The present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

No MeSH data available.


Related in: MedlinePlus

Heat map generated for ABC transporter families identified fromC. rogercresseyitranscriptome data reflecting gene expression values for all developmental stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4403882&req=5

Fig2: Heat map generated for ABC transporter families identified fromC. rogercresseyitranscriptome data reflecting gene expression values for all developmental stages.

Mentions: RNA-Seq analysis was carried out in order to evaluate the transcriptome profiles of ABC transporters during the developmental stages of C. rogercresseyi (Figure 2). From the expression profiles in the larval nauplius I-II stages, the subfamilies ABCA/B/C/D were highly regulated. In the infective copepodid stage, the ABCC/E/F/G/H transporters were upregulated. The ABCD/E/F subfamilies were highly regulated in chalimus I-II stages, while in the chalimus III-IV stages, the ABCA/B/C/F subfamilies were upregulated (Figure 2). Furthermore, differences in expression profiles were observed between female and male C. rogercresseyi individuals. The ABCD/F subfamilies were upregulated in females, while in males, the ABCB/C subfamilies evidenced high regulation (Figure 2).Figure 2


Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi.

Valenzuela-Muñoz V, Sturm A, Gallardo-Escárate C - Parasit Vectors (2015)

Heat map generated for ABC transporter families identified fromC. rogercresseyitranscriptome data reflecting gene expression values for all developmental stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403882&req=5

Fig2: Heat map generated for ABC transporter families identified fromC. rogercresseyitranscriptome data reflecting gene expression values for all developmental stages.
Mentions: RNA-Seq analysis was carried out in order to evaluate the transcriptome profiles of ABC transporters during the developmental stages of C. rogercresseyi (Figure 2). From the expression profiles in the larval nauplius I-II stages, the subfamilies ABCA/B/C/D were highly regulated. In the infective copepodid stage, the ABCC/E/F/G/H transporters were upregulated. The ABCD/E/F subfamilies were highly regulated in chalimus I-II stages, while in the chalimus III-IV stages, the ABCA/B/C/F subfamilies were upregulated (Figure 2). Furthermore, differences in expression profiles were observed between female and male C. rogercresseyi individuals. The ABCD/F subfamilies were upregulated in females, while in males, the ABCB/C subfamilies evidenced high regulation (Figure 2).Figure 2

Bottom Line: High transcript levels of the ABCB and ABCC subfamilies were evidenced.Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO. Box 160-C, Concepción, Chile. valevalenzuela@gmail.com.

ABSTRACT

Background: ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.

Methods: llumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.

Results: From the transcriptome database of C. rogercresseyi, 57 putative members of ABC protein sequences were identified and phylogenetically classified into the eight subfamilies described for ABC transporters in arthropods. Transcriptomic profiles for ABC proteins subfamilies were evaluated throughout C. rogercresseyi development. Moreover, RNA-Seq analysis was performed for adult male and female salmon lice exposed to the delousing drugs azamethiphos and deltamethrin. High transcript levels of the ABCB and ABCC subfamilies were evidenced. Furthermore, SNPs mining was carried out for the ABC proteins sequences, revealing pivotal genomic information.

Conclusions: The present study gives a comprehensive transcriptome analysis of ABC proteins from C. rogercresseyi, providing relevant information about transporter roles during ontogeny and in relation to delousing drug responses in salmon lice. This genomic information represents a valuable tool for pest management in the Chilean salmon aquaculture industry.

No MeSH data available.


Related in: MedlinePlus