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Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.

Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny PJ, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M - PLoS ONE (2015)

Bottom Line: We determined the effects of experimental conditions mimicking inflammation and hyperlipidemia on HRV and heart rate (HR) in relation to the immune, metabolic, and hormonal responses resulting from these interventions.During oral fat intake, ΔGLP-1 tended to correlate positively with ΔHR and inversely with ΔpNN50 and ΔRMSSD.In conclusion, suppression of vagal tone and sympathetic predominance during endotoxemia are linked to anti-inflammatory processes and lipid oxidation but not to insulin resistance, while weaker HRV changes in relation to the GLP-1 response are noted during oral fat load.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Diabetology, German Diabetes Center at Heinrich Heine University, Leibniz Center for Diabetes Research, Düsseldorf, Germany; Department of Endocrinology and Diabetology, University Hospital, Düsseldorf, Germany; German Center for Diabetes Research (DZD), Partner Düsseldorf, Düsseldorf, Germany.

ABSTRACT

Unlabelled: The autonomic nervous system (ANS) plays an important role in regulating the metabolic homeostasis and controlling immune function. ANS alterations can be detected by reduced heart rate variability (HRV) in conditions like diabetes and sepsis. We determined the effects of experimental conditions mimicking inflammation and hyperlipidemia on HRV and heart rate (HR) in relation to the immune, metabolic, and hormonal responses resulting from these interventions. Sixteen lean healthy subjects received intravenous (i.v.) low-dose endotoxin (lipopolysaccharide [LPS]), i.v. fat, oral fat, and i.v. glycerol (control) for 6 hours, during which immune, metabolic, hormonal, and five HRV parameters (pNN50, RMSSD, low-frequency (LF) and high-frequency (HF) power, and LF/HF ratio) were monitored and energy metabolism and insulin sensitivity (M-value) were assessed. LPS infusion induced an increase (AUC) in HR and LF/HF ratio and decline in pNN50 and RMSSD, while oral fat resulted in elevated HR and a transient (hours 1-2) decrease in pNN50, RMSSD, and HF power. During LPS infusion, ΔIL-1ra levels and ΔIL-1ra and ΔIL-1ß gene expression correlated positively with ΔLF/HF ratio and inversely with ΔRMSSD. During oral fat intake, ΔGLP-1 tended to correlate positively with ΔHR and inversely with ΔpNN50 and ΔRMSSD. Following LPS infusion, lipid oxidation correlated positively with HR and inversely with pNN50 and RMSSD, whereas HRV was not related to M-value. In conclusion, suppression of vagal tone and sympathetic predominance during endotoxemia are linked to anti-inflammatory processes and lipid oxidation but not to insulin resistance, while weaker HRV changes in relation to the GLP-1 response are noted during oral fat load.

Trial registration: ClinicalTrials.gov NCT01054989.

No MeSH data available.


Related in: MedlinePlus

Correlations of the changes in IL-1ra serum concentrations (A), IL-1ra expression (B), and IL-1ß expression (C) with RMSSD and low-frequency/high-frequency (LF/HF) ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake (D).
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pone.0124242.g003: Correlations of the changes in IL-1ra serum concentrations (A), IL-1ra expression (B), and IL-1ß expression (C) with RMSSD and low-frequency/high-frequency (LF/HF) ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake (D).

Mentions: Fig 3 illustrates the correlations of the changes in IL-1ra serum concentrations, IL-1ra expression, and IL-1ß expression with RMSSD and LF/HF ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake. The changes in GLP-1 concentrations following oral fat intake tended to be inversely correlated with pNN50 (P = 0.074) and RMSSD (P = 0.085), but not with the frequency domain measures.


Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.

Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny PJ, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M - PLoS ONE (2015)

Correlations of the changes in IL-1ra serum concentrations (A), IL-1ra expression (B), and IL-1ß expression (C) with RMSSD and low-frequency/high-frequency (LF/HF) ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403853&req=5

pone.0124242.g003: Correlations of the changes in IL-1ra serum concentrations (A), IL-1ra expression (B), and IL-1ß expression (C) with RMSSD and low-frequency/high-frequency (LF/HF) ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake (D).
Mentions: Fig 3 illustrates the correlations of the changes in IL-1ra serum concentrations, IL-1ra expression, and IL-1ß expression with RMSSD and LF/HF ratio following LPS infusion and correlations of the changes in GLP-1 with pNN50 and RMSSD after oral fat intake. The changes in GLP-1 concentrations following oral fat intake tended to be inversely correlated with pNN50 (P = 0.074) and RMSSD (P = 0.085), but not with the frequency domain measures.

Bottom Line: We determined the effects of experimental conditions mimicking inflammation and hyperlipidemia on HRV and heart rate (HR) in relation to the immune, metabolic, and hormonal responses resulting from these interventions.During oral fat intake, ΔGLP-1 tended to correlate positively with ΔHR and inversely with ΔpNN50 and ΔRMSSD.In conclusion, suppression of vagal tone and sympathetic predominance during endotoxemia are linked to anti-inflammatory processes and lipid oxidation but not to insulin resistance, while weaker HRV changes in relation to the GLP-1 response are noted during oral fat load.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Diabetology, German Diabetes Center at Heinrich Heine University, Leibniz Center for Diabetes Research, Düsseldorf, Germany; Department of Endocrinology and Diabetology, University Hospital, Düsseldorf, Germany; German Center for Diabetes Research (DZD), Partner Düsseldorf, Düsseldorf, Germany.

ABSTRACT

Unlabelled: The autonomic nervous system (ANS) plays an important role in regulating the metabolic homeostasis and controlling immune function. ANS alterations can be detected by reduced heart rate variability (HRV) in conditions like diabetes and sepsis. We determined the effects of experimental conditions mimicking inflammation and hyperlipidemia on HRV and heart rate (HR) in relation to the immune, metabolic, and hormonal responses resulting from these interventions. Sixteen lean healthy subjects received intravenous (i.v.) low-dose endotoxin (lipopolysaccharide [LPS]), i.v. fat, oral fat, and i.v. glycerol (control) for 6 hours, during which immune, metabolic, hormonal, and five HRV parameters (pNN50, RMSSD, low-frequency (LF) and high-frequency (HF) power, and LF/HF ratio) were monitored and energy metabolism and insulin sensitivity (M-value) were assessed. LPS infusion induced an increase (AUC) in HR and LF/HF ratio and decline in pNN50 and RMSSD, while oral fat resulted in elevated HR and a transient (hours 1-2) decrease in pNN50, RMSSD, and HF power. During LPS infusion, ΔIL-1ra levels and ΔIL-1ra and ΔIL-1ß gene expression correlated positively with ΔLF/HF ratio and inversely with ΔRMSSD. During oral fat intake, ΔGLP-1 tended to correlate positively with ΔHR and inversely with ΔpNN50 and ΔRMSSD. Following LPS infusion, lipid oxidation correlated positively with HR and inversely with pNN50 and RMSSD, whereas HRV was not related to M-value. In conclusion, suppression of vagal tone and sympathetic predominance during endotoxemia are linked to anti-inflammatory processes and lipid oxidation but not to insulin resistance, while weaker HRV changes in relation to the GLP-1 response are noted during oral fat load.

Trial registration: ClinicalTrials.gov NCT01054989.

No MeSH data available.


Related in: MedlinePlus