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Temperature during early development has long-term effects on microRNA expression in Atlantic cod.

Bizuayehu TT, Johansen SD, Puvanendran V, Toften H, Babiak I - BMC Genomics (2015)

Bottom Line: The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term.Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

View Article: PubMed Central - PubMed

Affiliation: University of Nordland, Faculty of Biosciences and Aquaculture, Post Box 1490, 8049, Bodø, Norway. ttb@uin.no.

ABSTRACT

Background: Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile.

Results: We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.

Conclusions: Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

No MeSH data available.


Related in: MedlinePlus

Long-term effects of elevated temperature in early ontogeny on miRNA expression in juvenile Atlantic cod depend on timing. Elevation of temperature during either embryo incubation or larval rearing (see Figure 6) affects different sets of miRNAs, with the exception of miR-192. Differential expression (up- or down-regulation) is given in relation to low temperature group, with the exception of miR-200a, where expression is differential in relation to high temperature group (see Additional file 7).
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Fig4: Long-term effects of elevated temperature in early ontogeny on miRNA expression in juvenile Atlantic cod depend on timing. Elevation of temperature during either embryo incubation or larval rearing (see Figure 6) affects different sets of miRNAs, with the exception of miR-192. Differential expression (up- or down-regulation) is given in relation to low temperature group, with the exception of miR-200a, where expression is differential in relation to high temperature group (see Additional file 7).

Mentions: Sequencing was performed separately for males and females, but no significant differences were found between the sexes within a treatment group. Fourteen miRNAs were differentially expressed among the treatment groups in pituitary, liver, and gonad (Figure 4, Additional files 4 and 7). No significant differential expression was found in brain.Figure 4


Temperature during early development has long-term effects on microRNA expression in Atlantic cod.

Bizuayehu TT, Johansen SD, Puvanendran V, Toften H, Babiak I - BMC Genomics (2015)

Long-term effects of elevated temperature in early ontogeny on miRNA expression in juvenile Atlantic cod depend on timing. Elevation of temperature during either embryo incubation or larval rearing (see Figure 6) affects different sets of miRNAs, with the exception of miR-192. Differential expression (up- or down-regulation) is given in relation to low temperature group, with the exception of miR-200a, where expression is differential in relation to high temperature group (see Additional file 7).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4403832&req=5

Fig4: Long-term effects of elevated temperature in early ontogeny on miRNA expression in juvenile Atlantic cod depend on timing. Elevation of temperature during either embryo incubation or larval rearing (see Figure 6) affects different sets of miRNAs, with the exception of miR-192. Differential expression (up- or down-regulation) is given in relation to low temperature group, with the exception of miR-200a, where expression is differential in relation to high temperature group (see Additional file 7).
Mentions: Sequencing was performed separately for males and females, but no significant differences were found between the sexes within a treatment group. Fourteen miRNAs were differentially expressed among the treatment groups in pituitary, liver, and gonad (Figure 4, Additional files 4 and 7). No significant differential expression was found in brain.Figure 4

Bottom Line: The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term.Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

View Article: PubMed Central - PubMed

Affiliation: University of Nordland, Faculty of Biosciences and Aquaculture, Post Box 1490, 8049, Bodø, Norway. ttb@uin.no.

ABSTRACT

Background: Environmental temperature has serious implications in life cycle of aquatic ectotherms. Understanding the molecular mechanisms of temperature acclimation and adaptation of marine organisms is of the uttermost importance for ecology, fisheries, and aquaculture, as it allows modeling the effects of global warming on population dynamics. Regulatory molecules are major modulators of acclimation and adaptation; among them, microRNAs (miRNAs) are versatile and substantial contributors to regulatory networks of development and adaptive plasticity. However, their role in thermal plasticity is poorly known. We have asked whether the temperature and its shift during the early ontogeny (embryonic and larval development) affect the miRNA repertoire of Atlantic cod (Gadus morhua), and if thermal experience has long-term consequences in the miRNA profile.

Results: We characterized miRNA during different developmental stages and in juvenile tissues using next generation sequencing. We identified 389 putative miRNA precursor loci, 120 novel precursor miRNAs, and 281 mature miRNAs. Some miRNAs showed stage- or tissue-enriched expression and miRNAs, such as the miR-17 ~ 92 cluster, myomiRs (miR-206), neuromiRs (miR-9, miR-124), miR-130b, and miR-430 showed differential expression in different temperature regimes. Long-term effect of embryonic incubation temperature was revealed on expression of some miRNAs in juvenile pituitary (miR-449), gonad (miR-27c, miR-30c, and miR-200a), and liver (let-7 h, miR-7a, miR-22, miR-34c, miR-132a, miR-192, miR-221, miR-451, miR-2188, and miR-7550), but not in brain. Some of differentially expressed miRNAs in the liver were confirmed using LNA-based rt-qPCR. The effect of temperature on methylation status of selected miRNA promoter regions was mostly inconclusive.

Conclusions: Temperature elevation by several degrees during embryonic and larval developmental stages significantly alters the miRNA profile, both short-term and long-term. Our results suggest that a further rise in seas temperature might affect life history of Atlantic cod.

No MeSH data available.


Related in: MedlinePlus